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51.
Jae-Suk Choi Bo-Bae Lee Sun Ju An Jae Hak Sohn Kwang Keun Cho In Soon Choi 《Fisheries Science》2012,78(5):1117-1123
The development of a simple and effective long-term storage protocol for harvested fresh Undaria pinnatifida is required to increase the market for U. pinnatifida products. We investigated the quality of U. pinnatifida after the application of various freezing and thawing methods to establish an optimum storage protocol. Four freezing temperatures (?40, ?30, ?20, and ?10?°C), four freezing conditions (in air without seawater and with 50, 75, and 100?% seawater), and four thawing times (6, 12, 18, and 24?h) were employed. Changes in color value, tensile strength, total bacterial count, and most probable number of coliforms were measured. The best method for preserving the overall quality of U. pinnatifida is freezing at ?30?°C with 50?% seawater and thawing in running tap water for 6?h. During retail display, changes in color, odor, tensile strength, and bacterial count of U. pinnatifida that had been freeze-thawed under optimum conditions were also measured, and a sensory evaluation was performed. There was no significant difference compared with the control within two?days at 10?°C. In conclusion, we developed a simple and effective long-term storage protocol that led to no significant loss of quality of fresh U. pinnatifida. 相似文献
52.
Jung Dae Lim Sung Ryul Lee Taeseong Kim Seon-A Jang Se Chan Kang Hyun Jung Koo Eunsoo Sohn Jong Phil Bak Seung Namkoong Hyoung Kyu Kim In Sung Song Nari Kim Eun-Hwa Sohn Jin Han 《Marine drugs》2015,13(2):1051-1067
Fucoidan is an l-fucose-enriched sulfated polysaccharide isolated from brown algae and marine invertebrates. In this study, we investigated the protective effect of fucoidan from Fucus vesiculosus on alcohol-induced murine liver damage. Liver injury was induced by oral administration of 25% alcohol with or without fucoidan (30 mg/kg or 60 mg/kg) for seven days. Alcohol administration increased serum aspartate aminotransferase and alanine aminotransferase levels, but these increases were suppressed by the treatment of fucoidan. Transforming growth factor beta 1 (TGF-β1), a liver fibrosis-inducing factor, was highly expressed in the alcohol-fed group and human hepatoma HepG2 cell; however, the increase in TGF-β1 expression was reduced following fucoidan administration. Treatment with fucoidan was also found to significantly reduce the production of inflammation-promoting cyclooygenase-2 and nitric oxide, while markedly increasing the expression of the hepatoprotective enzyme, hemeoxygenase-1, on murine liver and HepG2 cells. Taken together, the antifibrotic and anti-inflammatory effects of fucoidan on alcohol-induced liver damage may provide valuable insights into developing new therapeutics or interventions. 相似文献
53.
Soo‐Won Jang Muhammad Hamayun Eun‐Young Sohn Dong‐Hyun Shin Kil‐Ung Kim Byung‐Hyun Lee In‐Jung Lee 《植物养料与土壤学杂志》2008,171(2):181-186
Three rice (Oryza sativa L.) cultivars ( cv. Daesanbyeo, cv. Dongjinbyeo, cv. Junambyeo) were analyzed for endogenous gibberellin (GA) and jasmonic acid (JA) contents and their changes in response to elevated nitrogen (N) levels. The N fertilizer was applied in the form of urea [(NH2)2CO] at three rates (0, 36.8, 73.6 kg N ha–1). Plant growth (height and dry weight) was enhanced by the first N rate but not further enhanced by the highest rate. The endogenous GA contents were analyzed through high‐performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry–selected ion monitoring (GC‐MS‐SIM) while that of JA with GC‐MS‐SIM. They were analyzed one week after N application and were significantly increased with elevated N levels in all rice cultivars. The bioactive GA1 markedly increased, but its concentration differed in different rice cultivars. Similar fluctuations were observed for endogenous GA8, GA12, GA19, GA20, and GA53 in response to elevated N levels, showing that the rates of biosynthesis of GAs were differently affected by elevated N levels within different rice cultivars. The level of GA20, a precursor of GA1 biosynthesis, was not significantly increased, though GA19, a precursor of GA20, was found to be the most abundant GA type in all rice cultivars. Jasmonic acid content in the plants increased with the basic urea application (36.8 kg N ha–1), but significantly decreased with the double urea level (73.6 kg N ha–1). The results demonstrate that GA and JA are differentially affected in response to elevated N application in rice. 相似文献
54.
Sang-Kuk Kim Eun-Young Sohn Sang-Mo Kang In-Jung Lee 《Journal of Crop Science and Biotechnology》2009,12(1):43-46
Eight gibberellins (GAs) were identified and quantified in extracts of seed of the flax, Linum usitatissimum L. cv. Hwanamama, Hwabaekama by GC-MS-SIM and Kovats retention indices. Four of these gibberellins are members of the early-C13-hydroxylation
pathway (GA53, GA19, GA20, and GA1), and four are members of the non-C13-hydroxylation pathway (GA12, GA24, GA9, and GA4). Of these eight gibberellins, GA24 was more abundant in yellow-seeded flax (cv. Hwanamama) than in brown-seeded flax (cv. Hwabaekama), whereas GA9 was 8.3-fold more abundant in brown-seeded flax (cv. Hwabaekama) than in yellow-seeded flax (cv. Hwanamama). In both flax
cultivars, non-C13-hydroxylation pathway was the main route leading to endogenous accumulation of GA4 in the seed. 相似文献
55.
56.
Jeong KI Sohn YS Ahn K Choi C Han DU Chae C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):535-538
Lectin staining pattern in Peyer's patches of porcine ileum was studied using twenty one biotinylated-labeled lectins as cell markers which were visualized with avidin-biotin-peroxidase complex method (ABC). WGA appears to be a selective marker for tingible body macrophages in the porcine germinal centers. ConA may be a positive marker for the lymphoid tissues, whereas 9 lectins (DBA, SBA, SJA, s-WGA, PNA, ECL, UEA-I, PHA-E, and PHA-L) may be negative markers for the lymphoid tissues in all areas. 相似文献
57.
Jun-Sung Kim Tae-Jong Yoon Kyeong-Nam Yu Mi Suk Noh Minah Woo Byung-Geol Kim Kee-Ho Lee Byung-Hyuk Sohn Seung-Bum Park Jin-Kyu Lee Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):321-326
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism. 相似文献
58.
试验研究了添加蛋氨酸螯合铜(Met-Cu)、壳聚糖螯合铜(Chitosan-Cu)、酵母螯合铜(Yeast-Cu)对肉仔鸡生产性能、营养物质消化率、血清IGg水平、嗉囊腐蚀程度、肝脏和排泄物中铜(Cu)含量以及肌肉和血清中总胆固醇的水平的影响。240只肉仔鸡(Ross208)平均分配到4个处理组中:对照组、Met-Cu组、Chitosan-Cu组、Yeast-Cu组。3个螯合铜试验组日粮铜含量均为100mg/kg。每个处理组中设6个重复,每个重复10只鸡(5只公鸡 5只母鸡)。试验结果表明,螯合铜试验组增重和采食量均高于对照组。Met-Cu组增重显著高于对照组(P<0.05),Yeast-Cu组采食量显著高于对照组(P<0.05)。各处理组料重比明显不同,由低到高依次为Met-Cu组、对照组、Chitosan-Cu组和Yeast-Cu组。各组间干物质利用率差异显著(P<0.05),螯合铜的试验组干物质利用率显著高于对照组,其中Chitosan-Cu组最高。添加螯合铜提高了嗉囊腐蚀系数,Met-Cu组肝脏铜含量最高。添加螯合铜降低了胸肌和血清中总胆固醇水平,而提高了血清高密度脂蛋白(HDL)水平。试验结果表明,在肉仔鸡日粮中以螯合铜形式添加铜100mg/kg,可以提高增重、采食量和干物质利用率,其中Met-Cu效果最好。 相似文献
59.
An activity-guided fractionation procedure was used to identify the antioxidative components of the aerial parts of Saururus chinensis. The antioxidant activity was investigated with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical- and superoxide anion-scavenging assays. Three active compounds (flavonol glycosides) were identified. 相似文献
60.
Antibacterial effect of Dryopteris crassirhizoma against methicillin-resistant Staphylococcus aureus 总被引:1,自引:0,他引:1
Kwon DY Kang OH Choi JG Lee YS Oh YC Chae HS Lee GH Park PS Kim YC Sohn DH Park H Lee JH 《Fitoterapia》2007,78(6):430-433
Methanol extract and its fractions (hexane, EtOAc, n-BuOH, and H2O) of Dryopteris crassirhizoma were investigated for antibacterial activity against methicillin-resistant Staphylococcus aureus. The hexane fraction showed a good antibacterial activity against all tested strains. 相似文献