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51.
Soo‐Won Jang Muhammad Hamayun Eun‐Young Sohn Dong‐Hyun Shin Kil‐Ung Kim Byung‐Hyun Lee In‐Jung Lee 《植物养料与土壤学杂志》2008,171(2):181-186
Three rice (Oryza sativa L.) cultivars ( cv. Daesanbyeo, cv. Dongjinbyeo, cv. Junambyeo) were analyzed for endogenous gibberellin (GA) and jasmonic acid (JA) contents and their changes in response to elevated nitrogen (N) levels. The N fertilizer was applied in the form of urea [(NH2)2CO] at three rates (0, 36.8, 73.6 kg N ha–1). Plant growth (height and dry weight) was enhanced by the first N rate but not further enhanced by the highest rate. The endogenous GA contents were analyzed through high‐performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry–selected ion monitoring (GC‐MS‐SIM) while that of JA with GC‐MS‐SIM. They were analyzed one week after N application and were significantly increased with elevated N levels in all rice cultivars. The bioactive GA1 markedly increased, but its concentration differed in different rice cultivars. Similar fluctuations were observed for endogenous GA8, GA12, GA19, GA20, and GA53 in response to elevated N levels, showing that the rates of biosynthesis of GAs were differently affected by elevated N levels within different rice cultivars. The level of GA20, a precursor of GA1 biosynthesis, was not significantly increased, though GA19, a precursor of GA20, was found to be the most abundant GA type in all rice cultivars. Jasmonic acid content in the plants increased with the basic urea application (36.8 kg N ha–1), but significantly decreased with the double urea level (73.6 kg N ha–1). The results demonstrate that GA and JA are differentially affected in response to elevated N application in rice. 相似文献
52.
Sang-Kuk Kim Eun-Young Sohn Sang-Mo Kang In-Jung Lee 《Journal of Crop Science and Biotechnology》2009,12(1):43-46
Eight gibberellins (GAs) were identified and quantified in extracts of seed of the flax, Linum usitatissimum L. cv. Hwanamama, Hwabaekama by GC-MS-SIM and Kovats retention indices. Four of these gibberellins are members of the early-C13-hydroxylation
pathway (GA53, GA19, GA20, and GA1), and four are members of the non-C13-hydroxylation pathway (GA12, GA24, GA9, and GA4). Of these eight gibberellins, GA24 was more abundant in yellow-seeded flax (cv. Hwanamama) than in brown-seeded flax (cv. Hwabaekama), whereas GA9 was 8.3-fold more abundant in brown-seeded flax (cv. Hwabaekama) than in yellow-seeded flax (cv. Hwanamama). In both flax
cultivars, non-C13-hydroxylation pathway was the main route leading to endogenous accumulation of GA4 in the seed. 相似文献
53.
54.
Jeong KI Sohn YS Ahn K Choi C Han DU Chae C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):535-538
Lectin staining pattern in Peyer's patches of porcine ileum was studied using twenty one biotinylated-labeled lectins as cell markers which were visualized with avidin-biotin-peroxidase complex method (ABC). WGA appears to be a selective marker for tingible body macrophages in the porcine germinal centers. ConA may be a positive marker for the lymphoid tissues, whereas 9 lectins (DBA, SBA, SJA, s-WGA, PNA, ECL, UEA-I, PHA-E, and PHA-L) may be negative markers for the lymphoid tissues in all areas. 相似文献
55.
Jun-Sung Kim Tae-Jong Yoon Kyeong-Nam Yu Mi Suk Noh Minah Woo Byung-Geol Kim Kee-Ho Lee Byung-Hyuk Sohn Seung-Bum Park Jin-Kyu Lee Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):321-326
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism. 相似文献
56.
试验研究了添加蛋氨酸螯合铜(Met-Cu)、壳聚糖螯合铜(Chitosan-Cu)、酵母螯合铜(Yeast-Cu)对肉仔鸡生产性能、营养物质消化率、血清IGg水平、嗉囊腐蚀程度、肝脏和排泄物中铜(Cu)含量以及肌肉和血清中总胆固醇的水平的影响。240只肉仔鸡(Ross208)平均分配到4个处理组中:对照组、Met-Cu组、Chitosan-Cu组、Yeast-Cu组。3个螯合铜试验组日粮铜含量均为100mg/kg。每个处理组中设6个重复,每个重复10只鸡(5只公鸡 5只母鸡)。试验结果表明,螯合铜试验组增重和采食量均高于对照组。Met-Cu组增重显著高于对照组(P<0.05),Yeast-Cu组采食量显著高于对照组(P<0.05)。各处理组料重比明显不同,由低到高依次为Met-Cu组、对照组、Chitosan-Cu组和Yeast-Cu组。各组间干物质利用率差异显著(P<0.05),螯合铜的试验组干物质利用率显著高于对照组,其中Chitosan-Cu组最高。添加螯合铜提高了嗉囊腐蚀系数,Met-Cu组肝脏铜含量最高。添加螯合铜降低了胸肌和血清中总胆固醇水平,而提高了血清高密度脂蛋白(HDL)水平。试验结果表明,在肉仔鸡日粮中以螯合铜形式添加铜100mg/kg,可以提高增重、采食量和干物质利用率,其中Met-Cu效果最好。 相似文献
57.
An activity-guided fractionation procedure was used to identify the antioxidative components of the aerial parts of Saururus chinensis. The antioxidant activity was investigated with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical- and superoxide anion-scavenging assays. Three active compounds (flavonol glycosides) were identified. 相似文献
58.
Antibacterial effect of Dryopteris crassirhizoma against methicillin-resistant Staphylococcus aureus 总被引:1,自引:0,他引:1
Kwon DY Kang OH Choi JG Lee YS Oh YC Chae HS Lee GH Park PS Kim YC Sohn DH Park H Lee JH 《Fitoterapia》2007,78(6):430-433
Methanol extract and its fractions (hexane, EtOAc, n-BuOH, and H2O) of Dryopteris crassirhizoma were investigated for antibacterial activity against methicillin-resistant Staphylococcus aureus. The hexane fraction showed a good antibacterial activity against all tested strains. 相似文献
59.
Miryeong Sohn Franklin E. Barton Anna M. McClung Elaine T. Champagne 《Cereal Chemistry》2004,81(3):341-344
The use of the derivative method for near‐infrared (NIR) calibration was investigated to determine protein and amylose content in rice flour. Samples for two years, 1996 and 1999, were combined to give a wide range of the constituents for development of the calibration model. The NIR spectral data were transformed with Savitzky‐Golay derivative with multiplicative scatter correction. To develop the best derivative models, the polynomial fits (quadratic, cubic, and quartic), convolution intervals (3–11 points for protein, 3–17 points for amylose), and derivative orders (1st derivative D1; 2nd derivative D2) were investigated. For the protein analysis, all polynomial fits with 3–11 points were acceptable to develop both the D1 and D2 models. However, the three‐point quadratic and five‐point quartic fits were not acceptable for the D1 model, and the three‐point quadratic fit was not acceptable for D2. For the amylose analysis, the D1 model produced generally better results than D2. Higher convolution intervals were required for the D2 model, whereas the D1 model was not affected by convolution intervals. A quadratic (or cubic) fit with 17‐point convolution interval was acceptable for the amylose D2 model, and the quadratic fit with 5–11 points and cubic (or quartic) fit with 7–17 points were suitable for the D1 model. Based on the standard error of cross‐validation (SECV), the calibration models developed using data for two years resulted in good precision with an SECV of 0.23% for protein using four factors and an SECV of 1.0% for amylose using 10 factors. 相似文献
60.
Miryeong Sohn David S. Himmelsbach Sandra E. Kays Douglas D. Archibald Franklin E. Barton 《Cereal Chemistry》2005,82(6):660-665
The classification of cereals using near‐infrared Fourier transform Raman (NIR‐FT/Raman) spectroscopy was accomplished. Cereal‐based food samples (n = 120) were utilized in the study. Ground samples were scanned in low‐iron NMR tubes with a 1064 nm (NIR) excitation laser using 500 mW of power. Raman scatter was collected using a Ge (LN2) detector over the Raman shift range of 202.45~3399.89 cm‐1. Samples were classified based on their primary nutritional components (total dietary fiber [TDF], fat, protein, and sugar) using principle component analysis (PCA) to extract the main information. Samples were classified according to high and low content of each component using the spectral variables. Both soft independent modeling of class analogy (SIMCA) and partial least squares (PLS) regression based classification were investigated to determine which technique was the most appropriate. PCA results suggested that the classification of a target component is subject to interference by other components in cereal. The Raman shifts that were most responsible for classification of each component were 1600~1630 cm‐1 for TDF, 1440 and 2853 cm‐1 for fat, 2910 and 1660 cm‐1 for protein, and 401 and 848 cm‐1 for sugar. The use of the selected spectral region (frequency region) for each component produced better results than the use of the entire region in both SIMCA and PLS‐based classifications. PLS‐based classification performed better than SIMCA for all four components, resulting in correct classification of samples 85~95% of the time. NIR‐FT/Raman spectroscopy represents a rapid and reliable method by which to classify cereal foods based on their nutritional components. 相似文献