基于GIS的猕猴桃电商精准营销智能分析

旨在解决传统农产品营销模式中的种植计划粗放、营销市场针对性不足、无法充分挖掘市场等问题,实现猕猴桃电商精准营销.通过互联网等途径采集历年猕猴桃电商营销情况相关数据并建立猕猴桃地理信息数据库,结合GIS的地理数据空间分析与可视化技术,对猕猴桃营销大数据进行智能分析与深度挖掘.可以实现详细的数据统计、分析与总览等,对各地区...     

月季花瓣衰老过程中可溶性蛋白的SDS—PAGE分析

对月季花瓣衰老过程中可溶性蛋白的ＳＤＳ－ＰＡＧＥ进行了分析,结果表明,蛋白条带变化表现出３种情况：（１）从瓶插开始到结束蛋白条带基本保持稳定;（２）随花瓣衰老,蛋白条带逐渐减弱、消失;（３）随着花的衰老,有新的蛋白条带出现。对花进行ＡＢＡ或乙烯促衰处理,也导致相应的某些蛋白条带的变化,说明这些蛋白的变化可能与衰老有关。     

猪SENP1基因克隆、生物信息学分析与表达研究

试验旨在对猪SUMO特异性蛋白酶1(sentrin-specific protease 1,SENP1)基因CDS区进行克隆和生物信息学分析,并探讨SENP1基因在乙脑病毒(Japanese encephalitis virus,JEV)感染PK15细胞后的表达情况。根据GenBank中公布的猪SENP1基因预测序列(登录号:XM_013997974.2)设计特异性引物,通过RT-PCR和T/A克隆技术对猪SENP1基因CDS区序列进行克隆测序,并进行生物信息学分析,利用实时荧光定量PCR分析猪SENP1基因在JEV感染PK15细胞不同时间点(0、12、24、36、48 h)的表达情况。结果显示,猪SENP1基因CDS序列全长2 034 bp,共编码677个氨基酸。序列比对和系统进化树分析结果表明,猪SENP1蛋白序列和山羊、犬、人、牛的相似性分别为94.8%、94.2%、93.9%和93.8%,说明在这些物种中SENP1的保守性较强;猪与犬的SENP1分子亲缘关系较近。生物信息学分析结果显示,猪SENP1蛋白相对分子质量为76 825.76,等电点为8.53,体外半衰期为30 h,不稳定系数为53.59,总平均亲水指数为－0.622。SENP1蛋白不含信号肽序列,无跨膜结构域。二级结构分析结果显示,SENP1蛋白中α-螺旋、无规则卷曲、延伸链和β-转角分别占31.31%、46.68%、16.25%和5.76%。三级结构分析结果显示,猪SENP1蛋白中存在8个α-螺旋区和7个β-转角区。实时荧光定量PCR结果显示,猪SENP1基因在JEV感染的PK15细胞中呈现上调表达趋势,其中在感染后48 h SENP1基因表达量显著高于对照组(P<0.05)。本试验结果为后续深入研究SENP1基因功能提供了参考。     

Research on Lead-time Compression of Inventory Integrating Remanufacturing and Manufacturing System for the IOT Environment

The lead time compression is the core of supply chain management with time competition and the powerful source of competitive advantage of supply chain.As an emerging technology,The Internet of things ...     

FGF4在肺癌细胞体内和体外中表达差异的研究

FGF4已经被证明是癌基因,它涉及肿瘤的生长和转移,为了解FGF4的表达与肿瘤微环境的关系。我们利用FGF4抗体通过免疫组化对一名肺癌患者癌旁,癌组织,癌组织小鼠移植瘤,二次移植瘤以及原代培养的细胞爬片进行FGF4检测,探究其表达差异。通过对比癌组织与对照组（癌旁组织）,FGF4在癌巢中高表达;同样,移植瘤与二次移植瘤的癌巢中与癌旁组织比较,FGF4表达相对较高;但是将癌组织进行原代培养后,免疫组化检测细胞爬片FGF4,发现仅有5%±0.21%的肿瘤细胞表达FGF4,对照蛋白Cytokine作为肿瘤标记物,则在100%的肿瘤细胞中表达。研究提示免疫组化检测到FGF4在体内和体外表达不同,提示肿瘤细胞FGF4的表达与肿瘤微环境调控密切相关,肿瘤微环境对肿瘤细胞的FGF4的调控有着重要作用。     

湖南省R&D投入产出效率研究

以湖南省R&D投入产出活动为研究对象，采用数据包络分析法(DEA)的BCC模型，结合全国30个省市R&D投入产出数据，对2013年湖南省R&D投入产出效率进行比较分析，并根据实证结果提出相关对策及建议.     

Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid(SA) and methyl jasmonic acid(Me JA)supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control(p \ 0.01). Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.     

金中都公园的园林景观与历史文化展示

本文试以北京金中都公园设计和建设过程为例,论述如何将园林景观与历史文化的合理展示相结合。提出设计师的创作和实践应该与建设过程相配合,以匠人精神应对施工阶段的现场完形和设计提升。总结了设计项目从概念规划直至建成,设计师的全过程设计的重要性和必要性。探讨了景观与艺术专业的协同设计在文化表达和景观展示中的叠加效应。提出设计师对场地的挖掘和设计推敲,应该随着项目的施工建设进度并以现场设计为核心,并举例论述了现场调整对提升设计品质的重要作用。以期为公园改造和园林景观设计师的工作实践提供有益的参考和引导。     

Mycoplasma pneumoniae induces IL-1β production through activating NLRP3 inflammasome by ROS in RAW264.7 cells

AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells.     

农机车载电子设备用户界面框架设计

介绍了一种农机车载电子设备显示界面框架的设计与实现。基于C语言分析设计了界面框架的数据结构,使用分层的数据结构设计方法实现了多类型数据的显示需要和灵活的界面类型组合方式,以极少的开销实现了多级菜单。将内容数据与操作函数分离,解决了操作函数复用的问题,同时提高了程序的可维护性。提出的新型的界面显示框架实现了多种农业装备常见的界面应用类型,解决了小屏幕嵌入式系统显示界面移植性差问题。