Effect of salinity and arbuscular mycorrhizal fungi on growth and some physiological parameters of Citrus jambheri

Arbuscular mycorrhizal (AM) fungi alleviate the unfavorable effects of salinity stress on plant growth. A pot study was conducted to determine the effects of AM fungi and salt on growth and some physiological parameters of Citrus jambheri rootstock. Four levels of salinity (2, 4, 6, and 8 dS m^?1 as NaCl) and three mycorrhizal treatments (Glomus etunicatum, Glomus intraradices and non-mycorrhizal (NM) control) were used. As salinity increased, all measured characteristics of plants after 4.5-month growth except Na uptake, proline content, and electrolyte leakage decreased. Shoot dry weight and K uptake were significantly higher in G. intraradices-colonized seedlings than NM controls at all salinity levels. Root dry weight and shoot P uptake were significantly higher in G. etunicatum-colonized seedlings than NM controls at all salinity levels. G. intraradices-colonized seedlings had significantly higher proline content than NM controls and G. etunicatum-colonized seedlings at salinity levels of 4, 6 and 8 dS m^?1. The electrolyte leakage percentage was significantly lower in G. intraradices-colonized seedlings than NM controls at all salinity levels. The data demonstrated that mycorrhizal citrus seedlings exhibited greater tolerance to salt stress than NM seedlings and the enhanced proline content seems to be one of the mechanisms involved.     

Comparison of spatial interpolation techniques for mapping soil pH and salinity in agricultural coastal areas,northern Iran

This study attempted to characterize the spatial distributions of soil pH and electrical conductivity (EC_e) of coastal fields in the Miyandoroud region, northern Iran, for three soil layer depths by assessing spatial variability and comparing different interpolation techniques such as inverse distance weighting (IDW), ordinary kriging (OK), and conditional simulations (CS). Three soil composite samples were collected from 0–50, 50–100, and 100–150 cm depths at 105 sampling sites. At all three soil depths, pH and EC_e were best fitted by exponential and spherical models, respectively. Nugget effects were higher for soil EC_e data sets compared with soil pH at all three soil depths showing soil EC_e had a spatial variability in small distances. The prediction accuracy of the interpolation methods indicated that the minimum error for all data sets was achieved with the OK method, except for pH at 50–100 cm depth, and the CS technique revealed the largest error. The effect of different numbers of simulations (100, 500 and 1000) in the CS interpolation method resulted not in a realistic mapping for the soil EC_e and pH. Considering the high importance of irrigated agriculture in the Caspian Sea coastal areas, more subsoil salinity build-up and groundwater salinity monitoring plans are needed as a prerequisite for sustainable agricultural production systems of the future.     

Dry Matter and Nitrogen Accumulation and Root Morphological Characteristics of Two Clonal Selections of ‘Russet Norkotah’ Potato as Affected by Nitrogen Fertilization

New clonal selections with increased vine vigor and stress resistance have been identified for the potato cultivar ‘Russet Norkotah’. However, the importance of clonal variation in nitrogen (N) uptake and root morphological properties is not well known. The objective of this study was to determine the effect of N fertilization on dry matter and N accumulation and root morphological parameters of two clonal selections of ‘Russet Norkotah’. A field experiment was conducted in 2002 using the standard ‘Russet Norkotah’ clone (SRC) and Texas selection 112 (TX112) of ‘Russet Norkotah’, grown at 0 and 150 kg N ha^{? 1}. Whole plants were excavated at 54, 76, and 96 days after planting; partitioned into tubers, vines, roots, stolons, and fruits; and their dry matter and N accumulation were determined. Soil cores were obtained from 10 spatial locations relative to the plant, and used for determination of root length (RL), root length density (RLD), root average diameter (RAD), and root dry weight (RDW). Soil inorganic N content was also measured. Nitrogen fertilization increased tuber yield and dry matter and N accumulation. Fertilizer N application did not affect RL, RLD, or RDW, but resulted in a larger proportion of roots close to the top of the potato hill. Tuber yield and dry matter and N accumulation were similar for the two clonal selections. The TX112 clone, however, partitioned more dry matter and N to vines and less dry matter and N to tubers compared with the SRC clone. Soil nitrate concentration was significantly higher for SCR than for the TX112 clone in the fertilized treatment at 54 DAP, and was low and similar between clones thereafter. Root length and RLD were significantly higher for the TX112 clone compared with SRC, and both clones had a similar spatial distribution of roots. Under the conditions of this study where moisture and disease stress were limited and under a short growing season, the larger root system and increased vine vigor of the TX112 clone did not provide any advantage in terms of plant production as either dry-matter accumulation or tuber yield.     

Identification of a tractable model system and oxalic acid-dependent symptom development of the dollar spot pathogen Clarireedia jacksonii

Clarireedia jacksonii causes dollar spot disease of cool-season turfgrasses in the United States and produces the phytotoxin oxalic acid. The role of oxalic acid in host–pathogen interactions of C. jacksonii is unknown and there are multiple challenges to studying these interactions in natural turfgrass hosts. Consequently, identification of model plants to study C. jacksonii–host interactions and the role of oxalic acid in pathogenesis is necessary. Controlled environment inoculation assays were used to evaluate pathogenesis of C. jacksonii in various model plants and investigate the role of oxalic acid in symptom development. Observations at microscopic and macroscopic levels demonstrated that infection progressed similarly in all monocots tested (creeping bentgrass, wheat, barley, rice, Brachypodium distachyon) but not in the dicot Arabidopsis thaliana. Plant oxalic acid content increased from near zero to around 0.2–0.4 mM following inoculation with C. jacksonii in creeping bentgrass, barley, and wheat. Conversely, oxalic acid content remained near zero in A. thaliana and was not well correlated with inoculation in rice and B. distachyon, both of which had higher endogenous oxalic acid levels than other monocots. Time-course oxalic acid quantification experiments with creeping bentgrass and B. distachyon further supported a link between symptom development and in planta oxalic acid content and identified 48 hr postinoculation as a critical time-point for investigating the role of oxalic acid in C. jacksonii pathogenesis. These studies demonstrate that various monocots can serve as tractable model systems for studying C. jacksonii–host interactions and that increases in oxalic acid content are associated with C. jacksonii symptom development.     

Germination characteristics and diversity of soil seed banks and above-ground vegetation in disturbed and undisturbed oak forests

The destruction of natural ecosystems is an important issue in many parts of the world. In the west of Iran, a vast area of the Zagros Mountain range is covered by typical vegetation including several rare plant species, of which many are currently considered endangered by anthropogenic activities. Despite the important role of soil seed banks to help conserve these degraded plant communities, the floristic studies in the Zagros forests have only focused on above- ground vegetation. In this study, the characteristics of soil seed banks and above-ground vegetation were examined at two forest sites： an undisturbed control （Un） and a disturbed （D） site. The objectives of this study were 1） to investigate the diversity of above-ground vegetation and soil seed banks in disturbed and undisturbed forests, 2） to examine the soil properties and the germination characteristics of the soil seed bank in disturbed and undisturbed oak forests and 3） to estimate the potential of soil seed banks in the restoration of disturbed sites. The results show that soil properties between Un and D sites were significantly different with higher values of pH, NH4-N, N~o,, CEC, OC, clay and canopy percentage in the Un site than in the D site. The Simpson diversity, Margalef richness and evenness indices differed significantly between sites, either for the soil seed banks or the above-ground vegetation. After a period of 26 weeks, the germination speed and the mean daily number of germinants were significantly different between Un and D sites. Without other conservation measures, soil seed banks alone cannot result in a full recovery after severe disturbances in the oak forests of Zagros.     

Genotype × environment interactions for seed yield in rainfed winter safflower (Carthamus tinctorius L.) multi-environment trials in Iran

Evaluation of genotype × environment interaction (GEI) is an important component of the variety selection process in multi-environment trials. The objectives of this study were first to analyze GEI on seed yield of 18 spine safflower genotypes grown for three consecutive seasons (2008–2011) at three locations, representative of rainfed winter safflower growing areas of Iran, by the additive main effects and multiplicative interaction (AMMI) model, and second to compare AMMI-derived stability statistics with several stability different methods, and two stability analysis approaches the yield-stability (Ysi) and the GGE (genotype + genotype × environment) biplot that are widely used to identify high-yielding and stable genotypes. The results of the AMMI analysis showed that main effects due to genotype, environment, and GEI as well as first six interaction principle component axes (IPCA1 to 6) were significant (P < 0.01). According to most stability statistics of AMMI analyses, genotypes G5 and G14 were the most stable genotypes across environments. According to the adjusted stability variance (s²), the high-yielding genotype, G2, was unstable due to the heterogeneity caused by environmental index. Based on the definition of stable genotypes by regression method (b = 1, S _d ²  = 0), genotypes G11, G9, G14, G3, G12 and G13 had average stability for seed yield. Stability parameters of Tai indicated that genotype G5 had specific adaptability to unfavorable environments. The GGE biplot and the Ysi statistic gave similar results in identifying genotype G2 (PI-209295) as the best one to release for rainfed conditions of Iran. The factor analysis was used for grouping all stability parameters. The first factor separated static and dynamic concepts of stability, in which the Ysi and GGED (i.e., the distance from the markers of individual genotypes to the ideal genotype) parameters had a dynamic concept of stability, and the other remaining parameters had static concept of stability.     

The effect of feeding rations with different ratios of concentrate to alfalfa hay on blood hematological and biochemical parameters of farmed ostriches (Struthio camelus)

A two-month trial was conducted to investigate the effects of pelleting rations with 70:30, 65:35, and 60:40 concentrate to high-quality roughage (alfalfa hay) ratios on hematological and biochemical parameters of ostriches. A total of 18 seven-month-old male ostrich chicks, initially weighing an average of 60–70 kg, were distributed into three different outdoor paddocks at a stocking density of six birds per paddock. In the morning, blood collection was made from the wing vein after about 12 h of fasting. All rations resulted in no significant change in hematological parameters. However, with increasing alfalfa inclusion rate, the heterophil/lymphocyte ratio tended to be lower (P?=?0.089); whereas the values of hemoglobin, packed cell volume (PCV), and mean corpuscular hemoglobin (MCH) tended to be higher (P values 0.069, 0.072 and 0.094, respectively). In terms of plasma biochemical parameters, increasing the ratio of concentrate to alfalfa hay in ostrich diets resulted in significant (P?<?0.05) depression in the values of glucose (up to 20.2 %), total cholesterol (up to 19.2 %), creatine kinase (up to 27.9 %), and aspartate aminotransferase (up to 29.9 %). Additionally, the 65:35 or 60:40 rations caused a decrease in low-density lipoprotein cholesterol level (27.9 and 42 %, respectively; P?=?0.008) compared to the 70:40 ration. Without exception, no rations affected the values of leukocyte subsets, total protein, albumin, globulin, uric acid, alanine aminotransferase, gamma glutamyl transferase, triglycerides, high-density lipoprotein cholesterol, and very low density lipoprotein. Based on our results, the concentrate to alfalfa ratio of 60:40 could be suggested as optimum ratio for good health conditions of juvenile ostriches.     

Geostatistics-based spatial distribution of soil moisture and temperature regime classes in Mazandaran province,northern Iran

Soil moisture regime (SMR) and soil temperature regime (STR) classes as soil classification criterions are required by US Soil Taxonomy because they affect genesis, use, and management of soils. The lack of sufficient soil moisture and temperature data requires the characterization of the pedoclimate on the basis of climatic data processed by simulation models. This research was conducted to consider the new approach for SMR and STR mapping. The objectives of this study were to compare the four interpolation schemes including ordinary kriging (OK), cokriging (Co-K), inverse distance weighting, and conditional simulation for interpolating the monthly mean total precipitation (MMTP) and monthly mean air temperature (MMAT) and to apply the Java Newhall simulation model for the MMTP and MMAT predictive values at each node of 1 km² grids across the Mazandaran province, northern Iran, for delineating the SMR and STR classes. The semivariogram analyses showed moderate to strong spatial dependence of data sets. The accuracy of interpolators varied within months for both MMTP and MMAT data sets. In most cases, OK and Co-K methods had the highest accuracy with lower mean error, root mean square error, and higher concordance correlation coefficient. The predictive maps show high diversity of SMR classes including Aridic, Ustic, Udic, and Xeric. The STR classes comprise Mesic, Thermic, and Cryic regimes. Results herein indicated that geostatistical approaches can potentially provide the opportunity for mapping of SMR and STR classes in data scarce regions.     

Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages

This study assessed the changes of plasma vitamin A, E, and C and the lipid peroxidation status of sheep during breeding and pregnancy under drought conditions. The study was conducted on 105 cross-bred fat tailed ewes, 3–5 years old with body condition scores (BCS) of 2.5 to 3.5. The ewes were grazing on medium-to-low quality forages during summer and low quality forages within the succeeding months and had ad libitum access to a mixture of alfalfa hay (40%) and wheat straw (60%) in the afternoons. From 3 weeks before breeding till 1 month after the introduction of rams, 300 g of barley grain/head/day was offered to the ewes and then the supplemental grain was reduced to 100 g/head/day. For better synchronization of estrus cycles in ewes, they were isolated from the rams for at least 2 months and then kept in close proximity of the rams for 1 week before the introduction of the rams to the ewe flock. Then, whole blood samples were collected on days 1, 7, 21, and 120 after ram introduction. Vitamins A, E, and C were measured in plasma. Malondialdehyde (MDA) was measured in the hemolysate as a biomarker of lipid peroxidation. Plasma progesterone (P4) was measured in the samples of day 120 for assessing pregnancy status of the ewes. Vitamins A and C showed continuous and significant declines (P < 0.05) through days 1 to 120. Vitamin E declined only during the first 21 days of the study and remained almost constant till day 120. MDA concentration increased significantly at day 21. An elevated concentration of MDA was also detected at day 120. The difference between days 21 and 120 was not significant (P > 0.05). A positive correlation between vitamins E and C was detected at day 120 (r = 0.349, P < 0.01). Age and BCS did not affect the patterns of changes. Assuming that the ewes with P4 concentrations ≥2.5 ng/ml were pregnant, 95 out of 105 ewes (90.5%) were pregnant at day 120 of the study. Under the conditions of the present study with medium-to-low quality pastures as the main sources of feed, ewes of various ages and body conditions may suffer from oxidative stress during breeding and pregnancy.