Luteal Blood Flow and progesterone concentration during first and second postpartum estrous cycle in lactating dairy cows

The aim of the present study was to determine the differences in corpus luteum (CL) functionality between the first postpartum estrous cycle and the following cycle in lactating dairy cows. Luteal blood flow (LBF), luteal size and blood progesterone (P4) concentration were monitored during the first and second postpartum estrous cycle. During the first and second postpartum estrous cycle, the mean LBF value increased (p < .05) from early to late dioestrus, while it decreased rapidly in proestrus, resulting statistically lower (p < .05) than those registered in all previous phases. Statistically significant differences were not observed between overall LBF during first and second postpartum estrous cycle (p > .05). During the first postpartum estrous cycle, P4 blood concentrations showed a significant reduction (p < .05) from dioestrus to proestrus. A different trend of P4 concentrations was observed during the second postpartum estrous cycle, where mean P4 value registered in proestrus resulted statistically lower than those registered in the previous cycle phases (p < .05). The mean P4 concentration registered over the first postpartum estrous cycle resulted statistically lower (p < .05) than that registered during the second one. A significant correlation between P4 concentrations and LBF was registered only during the second postpartum estrous cycle. Results indicate that during the first postpartum estrous cycle, P4 concentration was independent of luteal blood flow and luteal size.     

Dogs' features strongly affect people's feelings and behavior toward them

Dogs are a strong appeal for people. The aim of this research was to assess whether different dog features affect people's feelings and behavior toward them. Three puppies (1 Labrador retriever, 1 golden retriever, and 1 Border collie), 7 average adult dogs (3 small, 2 medium, and 2 large), and 2 adult pit bulls (1 black and 1 brown) were involved. They were on the leash with handlers (12 girls) who were instructed to maintain a neutral posture of waiting. People who passed in front of the dog–handler couple were videorecorded and then interviewed through an 8-item questionnaire. Most passersby (81.7%) noticed the dog. Tenderness was higher for the puppies (64.9%) in comparison to the average adult dogs (35.4%) and pit bulls (10.8%) (χ² = 100.442; P = 0.000). Pit bulls left more passersby indifferent (36.3%), followed by the medium-sized dogs (26.5%) and then the small dogs (19.2%) (χ² = 37.268; P = 0.000). Fear was more common at the sight of a pit bull (10.8%), especially when compared with a puppy (2.3%) or a small dog (0.7%) (χ² = 17.723; P = 0.001). More respondents desired to interact with puppies (49.1%) than with pit bulls (22.5%; χ² = 11.133; P = 0.000), and they actually related with young dogs (20.5%) more than with the pit bulls (4.9%). A high statistical difference can be observed for the behavior of participants toward the different categories of dogs (χ² = 43.519; P = 0.000) and their handlers (χ² = 23.854; P = 0.000). In detail, passersby showed more interest toward puppies and interacted more with puppies and large dogs, and their handlers, compared with dogs showing different features.Some factors related to passersby could affect the dog catalysis effect. Women interacted with the handlers more than men did (12.1% vs. 7.3%; χ² = 3.980; P = 0.046), but no difference was observed for the number of interactions with dogs.Passersby handling a dog were more predisposed to interact with the experimental dog (45.6% vs. 7.5%; χ² = 139.606; P = 0.000) and his or her handler (43.5% vs. 5.7%; χ² = 64,526; P = 0.000). Data suggest that puppies and pit bulls are at the 2 extremes of the dog appeal–people axis, with average dogs in the middle.     

Population dynamics and spread of Unaspis yanonensis in Calabria, Italy

The occurrence of Unaspis yanonensis (Kuwana) (Hemiptera: Diaspididae) in Calabria (southern Italy) poses a serious threat to citrus cultivation. A regional survey was performed to record information about its distribution in local citrus orchards. Field population dynamics were also studied. The arrowhead scale, U. yanonensis, was recorded in the two main citrus cultivation areas of Calabria. In the investigated environments U. yanonensis completed two generations per year; a typical bi-modal oviposition was recorded. The arrowhead scale overwintered as second-instar males and pre-ovipositing females.     

Novel ferrihydrite sand coating process as a first step for designed technosols

Purpose

The production of technosols to remediate polluted or sealed urban soils to sustain new green areas is mainly empirical. For this, our research aims to contribute with the scientific knowledge base for purpose designing of technosols. Since iron minerals play an important role for many different functions of soils, we simplified a technique to incorporate and stabilize iron minerals in a substrate: a sand coated with an amorphous iron (hydr)oxide, a 2-line ferrihydrite (2L-FH).

Materials and methods

The 2L-FH was precipitated by neutralization of a concentrated FeCl₃ solution. The suspension was homogeneously mixed with the sand and the mixture was dried at 35 °C. The mechanical stability of the 21 2L-FH-coated sand was determined by shaking the aggregates in water for 0, 1, 10, 100, and 1 000 min. The degree of coating detachment and the properties of the coating after shaking were characterized through (a) Fe content, (b) zeta-potential and particle size of the detached particles, (c) the specific surface area (SSA) of the coated sand, and (d) its surface structure using scanning electron microscopy (SEM). A phosphate adsorption isotherm was performed to measure the P-sorption capacity of the shaken samples and to test the 2L-FH-quartz attachment stability against the surface charge reduction of the 2L-FH associated with P adsorption.

Results and discussion

A reduced Fe loss (30 %) and smaller sizes of the coating detached particles in the sample shaken for 1 000 min indicate that a fractioning and reattachment of these aggregates occurred during the agitation process, resulting in a smoother surface (SEM), and a larger SSA and P-sorption capacity. The coated shaken samples showed P-adsorption capacities (5.3–6.34 μmol P g^?1) comparable to high loadings of phosphate in soils, and low detachment of Fe (7–14 %) in spite of negativity surface charge increase.

Conclusions

The practical novel coating process along with the 1 000-min shaking produced a mechanical resistant and P-adsorptive effective coated sand that could sustain the needs of plants in further experiments.

     

Solomonsterol A,a Marine Pregnane-X-Receptor Agonist,Attenuates Inflammation and Immune Dysfunction in a Mouse Model of Arthritis

In the present study we provide evidence that solomonsterol A, a selective pregnane X receptor (PXR) agonist isolated from the marine sponge Theonella swinhoei, exerts anti-inflammatory activity and attenuates systemic inflammation and immune dysfunction in a mouse model of rheumatoid arthritis. Solomonsterol A was effective in protecting against the development of arthritis induced by injecting transgenic mice harboring a humanized PXR, with anti-collagen antibodies (CAIA) with beneficial effects on joint histopathology and local inflammatory response reducing the expression of inflammatory markers (TNFα, IFNγ and IL-17 and chemokines MIP1α and RANTES) in draining lymph nodes. Solomonsterol A rescued mice from systemic inflammation were assessed by measuring arthritis score, CRP and cytokines in the blood. In summary, the present study provides a molecular basis for the regulation of systemic local and systemic immunity by PXR agonists.     

Ultrasonographic differentiation between portal venous and parenchymal gas may be important for the prognosis of canine and feline hepatic emphysema: 37 cases

The aim of this retrospective, cross‐sectional, study was to evaluate clinical findings and outcomes for different ultrasonographic patterns of hepatic emphysema in dogs and cats. Dogs and cats with an ultrasonographic diagnosis of hepatic emphysema and a known outcome, from January 2010 to January 2018, were enrolled. The following data were recorded from medical and ultrasonographic records: ultrasonographic patterns of hepatic emphysema (parenchymal, portal venous, biliary), clinical signs, laboratory findings, and outcomes (favorable, poor). A total of 33 dogs and four cats met the inclusion criteria. Among these, 23 cases were classified as hepatic portal venous gas, 10 as parenchymal emphysema, and four as biliary emphysema. Clinical diagnosis categories were as follows: infection/sepsis (9), gastro‐intestinal disease (9), iatrogenic (9), trauma (5), and liver neoplasia (5). An increase in serum liver enzymes was significantly associated with parenchymal emphysema (P = .03). Other clinical and laboratory findings were not associated with the type of hepatic emphysema. Hepatic portal venous gas was mostly transient in patients with ultrasonographic follow‐up. The overall mortality was 40.5%. A significant difference was found between mortality by portal venous gas (21.7%) and mortality by parenchymal emphysema (90%) (P = .003). In conclusion, the ultrasonographic differentiation of hepatic emphysema between hepatic portal venous gas and parenchymal emphysema may be important for the prognosis of hepatic emphysema. The presence of parenchymal emphysema may be a poor prognostic indicator, while hepatic portal venous gas may be more benign. However, ultrasound findings should be carefully evaluated in the context of clinical findings.