创造客厅的舒适优雅

如果想要装饰你的家,无论是完全重新装修,或者只是在外观上有所改变,都不妨遵循国际设计巨星提摩西·克里根的设计诀窍。从色彩、家具布置到灯光及配饰等一步步营造亲密的谈话空间,让舒适与优雅的设计元素出现在自己家中。     

A role for skin gammadelta T cells in wound repair

Gammadelta T cell receptor-bearing dendritic epidermal T cells (DETCs) found in murine skin recognize antigen expressed by damaged or stressed keratinocytes. Activated DETCs produce keratinocyte growth factors (KGFs) and chemokines, raising the possibility that DETCs play a role in tissue repair. We performed wound healing studies and found defects in keratinocyte proliferation and tissue reepithelialization in the absence of wild-type DETCs. In vitro skin organ culture studies demonstrated that adding DETCs or recombinant KGF restored normal wound healing in gammadelta DETC-deficient skin. We propose that DETCs recognize antigen expressed by injured keratinocytes and produce factors that directly affect wound repair.     

Genetic structure of the purebred domestic dog

We used molecular markers to study genetic relationships in a diverse collection of 85 domestic dog breeds. Differences among breeds accounted for approximately 30% of genetic variation. Microsatellite genotypes were used to correctly assign 99% of individual dogs to breeds. Phylogenetic analysis separated several breeds with ancient origins from the remaining breeds with modern European origins. We identified four genetic clusters, which predominantly contained breeds with similar geographic origin, morphology, or role in human activities. These results provide a genetic classification of dog breeds and will aid studies of the genetics of phenotypic breed differences.     

Use of plant extracts in the control of common bean anthracnose

Three hundred (300) methanol extracts of barks, leaves, flowers and stems of 200 plant species from Alto Rio Grande and Vale do São Francisco regions, State of Minas Gerais, Brazil, were prepared and submitted to an in vitro growth test with the fungus Colletotrichum lindemuthianum, causal agent of anthracnose in common bean. Extracts from 13 plant species (Astronium fraxinifolium, Inga marginata, Malva sylvestris, Matayba elaeagnoides, Miconia argyrophylla, Myrcia fallax, Ocimum gratissimum, Origanum vulgare, Rollinia emarginata, Siparuna arianeae, Styrax pohlii, Tabebuia serratifolia and Trichilia pallida) presented antifungal activity and were used in other in vitro assays. Extracts from M. argyrophylla, M. fallax, O. vulgare, S. arianeae and S. pohlii were the most promising for the inhibition of the mycelial growth while the extracts of M. argyrophylla, M. elaeagnoides and O. gratissimum presented the best results for the inhibition of conidial germination. Under greenhouse conditions, the extracts of M. argyrophylla and O. vulgare caused the greatest reductions (41.82% and 37.65%, respectively) in disease severity when a local effect assay was carried out. In the systemic effect assay, also in a greenhouse, the most promising extracts were those from I. marginata, M. argyrophylla, M. fallax, M. sylvestris, O. gratissimum, O. vulgare and S. arianeae, which reduced the severity of the anthracnose to values below 35% of the observed for the control. Therefore, future studies with these plant species should be carried out to develop new products to control the common bean anthracnose.     

Capture efficiency of a multi-species survey trawl for Snow Crab (Chionoecetes opilio) in the Newfoundland region

An experiment was carried out during September of 2001 to estimate the relative catching efficiency of the NAFC's (Northwest Atlantic Fisheries Centre) standard survey bottom trawl, the Campelen 1800 shrimp trawl, for Snow Crab (Chionoecetes opilio). Secondary trawls were mounted underneath the main trawl directly behind the footgear to estimate escapement of crab passing underneath the main trawl. Overall, the capture efficiency of the survey trawl was found to be much lower than 1.0 and highly variable, with variability being strongly related to substrate type and Snow Crab size. The probability of capture monotonically increased with crab size, and was higher on soft than on hard substrates. Three substrate-specific capture efficiency functions were defined; (1) efficiency was highest (about 0.73–0.95), and least dependent on crab size, on the softest mud substrate; (2) efficiency was lower (about 0.39–0.70) and linearly related to crab size throughout a soft mud–sand substrate; and (3) efficiency was lowest (about 0.05–0.33) on a variety of hard substrates within relatively shallow water. Our models over-estimated true efficiency because secondary trawls were not fully efficient, i.e., crab escaped underneath their footgear. Nevertheless, our experiment demonstrated that substrate type can affect capture efficiency and annual changes in trawl configuration or in Snow Crab distribution in relation to substrate type will affect survey biomass estimates. Additional studies using various approaches could provide a basis for standardizing survey catches for varying capture efficiency effects and refining survey-based estimates of biomass and exploitation rates of Snow Crab.     

A morphological study off a thyroid carcinoma in a medaka, Oryzias latipes (Temminck & Schlegel)

A large mass protruding beyond the left operculum was found in an adult male medaka, Oryzias latipes (Temminck & Schlegel). Histologically, the mass was mainly composed of thyroid follicles measuring up to 292 μm in diameter. The follicular epithelium consisted of cuboidal or squamous cells. The follicles lacked colloidal material in the lumen. The thyroid tissue infiltrated into the kidney. Electron microscopically, the tumour cells were characterized by dilation and vesiculation of rough endoplasmic reticulum, which extended throughout the cytoplasm, and an irregular outline of the nucleus. Mitotic figures and partly degenerate cells were frequently observed. Based on the above features, the tumour was diagnosed as a follicular carcinoma of the thyroid, the first reported case in the medaka.     

Leading the pack: Best practices in comparative canine cancer genomics to inform human oncology

Pet dogs develop spontaneous cancers at a rate estimated to be five times higher than that of humans, providing a unique opportunity to study disease biology and evaluate novel therapeutic strategies in a model system that possesses an intact immune system and mirrors key aspects of human cancer biology. Despite decades of interest, effective utilization of pet dog cancers has been hindered by a limited repertoire of necessary cellular and molecular reagents for both in vitro and in vivo studies, as well as a dearth of information regarding the genomic landscape of these cancers. Recently, many of these critical gaps have been addressed through the generation of a highly annotated canine reference genome, the creation of several tools necessary for multi-omic analysis of canine tumours, and the development of a centralized repository for key genomic and associated clinical information from canine cancer patients, the Integrated Canine Data Commons. Together, these advances have catalysed multidisciplinary efforts designed to integrate the study of pet dog cancers more effectively into the translational continuum, with the ultimate goal of improving human outcomes. The current review summarizes this recent progress and provides a guide to resources and tools available for comparative study of pet dog cancers.     

Practical implications of using non-relational databases to store large genomic data files and novel phenotypes

The objective of our study was to provide practical directions on the storage of genomic information and novel phenotypes (treated here as unstructured data) using a non-relational database. The MongoDB technology was assessed for this purpose, enabling frequent data transactions involving numerous individuals under genetic evaluation. Our study investigated different genomic (Illumina Final Report, PLINK, 0125, FASTQ, and VCF formats) and phenotypic (including media files) information, using both real and simulated datasets. Advantages of our centralized database concept include the sublinear running time for queries after increasing the number of samples/markers exponentially, in addition to the comprehensive management of distinct data formats while searching for specific genomic regions. A comparison of our non-relational and generic solution, with an existing relational approach (developed for tabular data types using 2 bits to store genotypes), showed reduced importing time to handle 50M SNPs (PLINK format) achieved by the relational schema. Our experimental results also reinforce that data conversion is a costly step required to manage genomic data into both relational and non-relational database systems, and therefore, must be carefully treated for large applications.     

A mechanical study of ligature security of commercially available pre-tied ligatures versus hand tied ligatures for use in equine laparoscopy

OBJECTIVE: To determine a hand-tied ligature knot configuration, suture size, and suture type that would be an acceptable substitute to commercially available ligature knots for use in equine laparoscopic surgery. STUDY DESIGN: Three-factor ANOVA with the fixed effects being suture type, suture size, and knot type. The dependent variable was ligature security (load to failure). METHODS: Commercially available Endoloop and 4 hand-tied slipknot ligatures were compared with a 4-layer square knot. The hand tied ligature knots tested were the 4S modified Roeder knot, the Brooks knot, the modified Roeder knot, and the Weston knot. Two suture sizes (0, 2) of each of 2 suture types (polydioxanone, polyglactin 910) were tested with each hand tied knot. Two types (polydioxanone, polyglactin 910) of size 0 Endoloop were tested. Twenty repetitions of each knot suture type were tested for force to yield on a materials testing machine. Statistical analysis consisted of a 3-way ANOVA with individual comparisons made using the Bonferroni method. Significance was P< or = .05. RESULTS: Only the 4S modified Roeder knot in 2 polydioxanone (103 N) was comparable with the breaking strength of the square knots (157 N). All other knot suture combinations tested were significantly weaker than the square knot (range, 2-18 N). CONCLUSION: For equine laparoscopy, a 2 polydioxanone 4S modified Roeder knot would be an acceptable alternative to the commercially available Endoloop. CLINICAL RELEVANCE: Laparoscopic slip knots are commonly used in equine laparoscopic surgery. Various knot configuration and suture size and type combinations will affect the security of the slip knot.