Review and analysis of the potential for glyphosate to interact with the estrogen,androgen and thyroid pathways

Glyphosate was recently evaluated for its potential to interact with the estrogen, androgen and thyroid (EAT) hormone pathways, including steroidogenesis, under the United States Environmental Protection Agency's (USEPA) Endocrine Disruptor Screening Program (EDSP), then by Germany, the rapporteur Member State who led the European Annex 1 renewal for glyphosate, and then by the European Food Protection Agency (EFSA) also as part of the Annex 1 renewal for glyphosate. Under the EDSP, 11 Tier 1 assays were run following the USEPA's validated 890‐series test guidelines and included five in vitro and six in vivo assays to evaluate the EAT pathways. Steroidogenesis was evaluated as part of the estrogen and androgen pathways. An up‐to‐date critical review has been conducted that considered results from the EDSP Tier 1 battery, guideline regulatory studies and an in‐depth analysis of the literature studies that informed an endocrine assessment. A strength of this evaluation was that it included data across multiple levels of biological organization, and mammalian and nonmammalian test systems. There was strong agreement across the in vitro and in vivo Tier 1 battery, guideline studies and relevant literature studies, demonstrating that glyphosate does not interact with EAT pathways including steroidogenesis. Based on an analysis of the comprehensive toxicology database for glyphosate and the literature, this review has concluded that glyphosate does not have endocrine‐disrupting properties through estrogen, androgen, thyroid and steroidogenic modes of action. © 2020 Society of Chemical Industry     

Establishment techniques affect productivity,nutritive value and atmospheric N2 fixation of two sunn hemp cultivars

Nitrogen fertilization is a common practice for sustaining forage production in forage systems in southeastern United States. Warm-season annual legumes may be an alternative forage to warm-season perennial grasses that do not require N fertilization. Sunn hemp (Crotalaria juncea L.) is a fast-growing, warm-season annual legume native to India and Pakistan. The objective of this 2-year study was to assess the herbage accumulation (HA), atmospheric N₂ fixation (ANF) and nutritive value of sunn hemp. Treatments were the factorial arrangement of two sunn hemp cultivars (“Crescent Sun” and “Blue Leaf”), three seeding rates (17, 28 and 39 kg seed/ha) and seed inoculation (inoculated or non-inoculated seeds), distributed in a randomized complete block design with four replicates. Crescent sun had greater HA (3,218 vs. 1764 kg DM/ha) and ANF (41 vs. 25 kg N/ha). Blue leaf had greater crude protein (CP) (188 vs. 176 g/kg) and in vitro digestible organic matter (IVDOM) concentrations (564 vs. 531 g/kg) than crescent sun. Non-inoculated seed had greater CP than inoculated seed, 188 and 177 g/kg, respectively, and inoculation did not affect HA. Intermediate seeding rate (28 kg/ha) decreased HA (2002 kg DM/ha), while HA from high and low seeding rates (17 and 39 kg/ha, respectively) did not differ (2,863 and 2,615 kg DM/ha respectively). Planting non-inoculated crescent sun at 17 kg/ha seeding rate is a feasible management practice to produce sunn hemp in subtropical regions; however, inoculation should always be recommended for proper establishment.     

Comparison of Chemical Composition and Biological Properties of Essential Oils Obtained by Hydrodistillation and Steam Distillation of Laurus nobilis L.

Plant Foods for Human Nutrition - The purpose of this study was to compare the yield, chemical composition, antimicrobial and antioxidant properties of essential oils isolated from leaves of Laurus...     

Evaluating methods for identifying large mammal road crossing locations: black bears as a case study

Landscape Ecology - Roads have several negative effects on large mammals including restricting movements, isolating populations, and mortality due to vehicle collisions. Where large mammals...     

Planktonic foraminifera of the California Current reflect 20th-century warming

It is currently unclear whether observed pelagic ecosystem responses to ocean warming, such as a mid-1970s change in the eastern North Pacific, depart from typical ocean variability. We report variations in planktonic foraminifera from varved sediments off southern California spanning the past 1400 years. Increasing abundances of tropical/subtropical species throughout the 20th century reflect a warming trend superimposed on decadal-scale fluctuations. Decreasing abundances of temperate/subpolar species in the late 20th century indicate a deep, penetrative warming not observed in previous centuries. These results imply that 20th-century warming, apparently anthropogenic, has already affected lower trophic levels of the California Current.     

Hemoxygenase-2 is an oxygen sensor for a calcium-sensitive potassium channel

Modulation of calcium-sensitive potassium (BK) channels by oxygen is important in several mammalian tissues, and in the carotid body it is crucial to respiratory control. However, the identity of the oxygen sensor remains unknown. We demonstrate that hemoxygenase-2 (HO-2) is part of the BK channel complex and enhances channel activity in normoxia. Knockdown of HO-2 expression reduced channel activity, and carbon monoxide, a product of HO-2 activity, rescued this loss of function. Inhibition of BK channels by hypoxia was dependent on HO-2 expression and was augmented by HO-2 stimulation. Furthermore, carotid body cells demonstrated HO-2-dependent hypoxic BK channel inhibition, which indicates that HO-2 is an oxygen sensor that controls channel activity during oxygen deprivation.