Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse

Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism.     

番鸭细小病毒和鹅细小病毒二联弱毒细胞苗的研究

应用适应于番鸭胚成纤维细胞(MDEFC)上繁殖,并产生细胞病变的鹅细小病毒(GPV)弱毒疫苗株与番鸭细小病毒(MPV)弱毒疫苗,按适当比例混合,试制了MPV-GPV二联弱毒细胞苗,并测定了各项免疫指标.结果显示试制出的二联苗对1日龄雏番鸭具有良好的安全性和遗传稳定性;免疫后5 d和7 d攻击GPV强毒,保护率分别为75%和100%,同时免疫后7 d血清中MPV-胶乳凝集抑制(LPAI)抗体效价均大于21,21～28 d抗体达高峰,有效免疫期超过60 d.疫苗于-20℃保存期大于12个月.上述结果表明,二联弱毒细胞苗安全有效.     

Evaluation of the colonization capabilities of Salmonella Enteritidis in Quails using an RT-PCR approach

We used a real-time PCR assay and indirect fluorescent antibody (IFA) assay to detect genomic DNA of Salmonella Enteritidis in the internal organs of quails after an oral challenge. The results showed that S. Enteritidis was detected in all the samples at different time points. This study will assist a future understanding of the pathogenesis of S. Enteritidis.     

重庆雪宝山自然保护区生态安全设计

介绍了重庆雪宝山自然保护区的基本情况、保护区自然资源的主要特色及其科学价值，论述了保护区生态安全设计的指导思想和原则，略述了该设计的主要内容。     

潍坊地区奶牛隐性乳腺炎感染率调查及防治效果考核

乳腺炎作为奶牛的高发病,是危害奶牛业生产、限制效益提高的主要疾病之一。据国外报道,奶牛乳腺炎的发病率约25%-60%,国内报道则更高,阳性率为48.2%-69.7%。乳腺炎包括临床型和隐性两种。据报道,隐性乳腺炎造成牛奶产量下降19.5%-43.4%,占每头牛年生产力的10%-11%,造成的经济损失比临床型乳腺炎更为严重。乳腺炎不仅影响产奶量,而且使乳的品质降低,危害人类健康。近年来国内外为降低乳腺炎发病率,投入了大量的人、财、物力,但效果并不令人满意。近年来采用以提高乳牛机体免疫功能与遗传分析相结合的乳腺炎综合防治技术,取得良好效果。笔者于…     

贵州省生物天然气发展现状及农作物秸秆资源潜力

对贵州省现阶段生物天然气工程发展的现状进行了阐述,研究了贵州省发展生物天然气存在的问题和困难,并统计了各市州农作物秸秆资源可收集量,计算出了生物天然气的生产潜力,以期为生物天然气的制备和利用提供参考。     

Dietary Supplementation with Microencapsulated Lutein Improves Yolk Color and Lutein Content in Fresh and Cooked Eggs of Laying Hens

This study was conducted to compare the efficacy of diet supplemented with non-microencapsulated lutein (NL) and microencapsulated lutein (ML) in laying hens. A total of 270 Hy-line Brown laying hens (54 weeks old) were allocated to three groups with six replicates of 15 hens and were adapted to a wheat-soybean meal basal diet for two weeks. Next, the control birds were fed the basal diet, and the test birds were fed the basal diet supplemented with 600 mg/kg NL (12 mg/kg available lutein) or 90.1 mg/kg ML (10 mg/kg available lutein) for 35 days. Supplementation of lutein did not affect the productive performance of laying hens, but improved (P<0.05) the yolk color and red/green value (a*), with eggs from the ML group displaying improved color and a* values from the 15^th day of the experimental period. The blue/yellow value (b*) for the yolk showed an increase (P<0.05) through both NL and ML supplements. The yolk color of fried and boiled eggs and a* value of the yolk in fried eggs were improved (P<0.05) only through ML supplemented diet. Both NL and ML supplements resulted in lower (P<0.05) lightness and higher (P<0.05) a* values of yolk in boiled eggs, as well as higher (P<0.05) b* values in fried and boiled eggs. Yolk lutein content in fresh, fried, and boiled eggs was increased (P<0.05) in NL and ML groups with the latter being higher. In conclusion, ML improved yolk pigmentation and lutein retention in laying hens better than NL.     

水产动物诱食剂的研究进展

在水产养殖中 ,很多饲料投放后未被动物采食就沉入了水底 ,不但浪费了饲料 ,而且还污染了水质和提高了饲料成本 ,而水产动物诱食剂可以缓解这些问题。     

内蒙古呼伦贝尔地区羊源多杀性巴氏杆菌荚膜血清群分子流行病学调查

近年来,羊多杀性巴氏杆菌病在内蒙古自治区呼伦贝尔市时有发生。为了解呼伦贝尔市羊源多杀性巴氏杆菌荚膜血清型流行状况,通过建立多杀性巴氏杆菌分子生物学诊断方法,以羊病变组织为研究对象,对呼伦贝尔市羊源多杀性巴氏杆菌,进行荚膜血清群分子流行病学调查,同时应用K-B纸片琼脂扩散法,对分离菌株进行药物敏感性试验,以找到针对本地区流行株的敏感药物。结果显示:从采集的35份病羊肺组织病料中,分离出12株B群、9株D群多杀性巴氏杆菌,未分离出其他血清群;分离菌株对青霉素、链霉素耐药率最高,对氯霉素、四环素、诺氟沙星、环丙沙星敏感。结果表明,呼伦贝尔市流行的羊源多杀性巴氏杆菌以B群、D群为主,且分离菌株对青霉素、链霉素产生了较高的耐药性,需指导和监管抗菌药物的合理使用。本研究查清了本地区流行的优势多杀性巴氏杆菌血清群,找到了针对性敏感药物,这为该市羊巴氏杆菌病防控提供了有效的技术支撑,也对多杀性巴氏杆菌病流行病学监测和基因多样性研究奠定了基础。     

北大营示范场黑麦草引种与品比试验研究

利用1998年2－11月在云南省北大营示范场引种的10个黑麦草品种的观测资料，统计分析了在高、中、低3种施肥水平下黑麦草的累计产量、月平均产量及其生长速度的变化规律，研究了影响产量的主导因子及其监测模型。结果表明，在10个品种中，伯生（Bison Intermdiate）及安巴瑟（Embassy）具有良好的生产性能。黑麦草产量主要受气候、土壤肥力及品种等因素的影响，其中测草盘高度、降水量、蒸发量、日照时数和牧草生长天数等5个因子，可以较好地模拟、评估黑麦草产量的变化动态。