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排序方式: 共有299条查询结果,搜索用时 15 毫秒
191.
Dana Skibbe En-Min Zhou Bruce H Janke 《Journal of veterinary diagnostic investigation》2004,16(1):86-89
A commercial indirect swine influenza virus (SIV) H1N1 enzyme-linked immunosorbent assay (ELISA) was compared with the hemagglutination inhibition (HI) assay by testing 72 samples from experimentally infected pigs and 780 field samples of undefined SIV status. The HI assay was performed using SIV isolates A/Swine/IA/73 for H1N1 and A/Swine/IA/8548-1/98 for H3N2. The ELISA used an SIV isolated in 1988. The results showed that HI and ELISA detected an antibody in 11 and 6, respectively, of 72 serum samples collected from pigs experimentally infected with a 1992 SIV isolate (A/Swine/IA/40776/92). The presence of antibodies in these experimental samples was confirmed by HI tests in which all 72 samples were positive against the homologous virus, a more recent H1N1 SIV isolate (A/Swine/NVSL/01) supplied by National Veterinary Services Laboratories, Ames, Iowa, and a 1999 H1N1 isolate currently used in a commercial vaccine. On testing 780 field samples, an overall agreement of 85.5% was generated between the HI and ELISA. This study demonstrated that the ELISA is a useful serodiagnostic screening test at herd level for detecting swine antibodies against SIV. However, a new SIV isolate representing current SIV strains circulating in the field is needed to replace the older isolates used in the HI and ELISA to increase the test accuracy for serodiagnosis of SIV. 相似文献
192.
Erez Hanael Shelly Baruch Orit Chai Liron Lishitsky Tal Blum Kira Rapoport Marco Ruggeri Zahi Aizenberg Dana Peery Nina Meyerhoff Holger Andreas Volk Steven De Decker Andrea Tipold Wolfgang Baumgaertner Alon Friedman Merav Shamir 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):606-617
193.
Aubert I Abrams-Ogg AC Sylvestre AM Dyson DH Allen DG Johnstone IB 《Canadian journal of veterinary research》2011,75(1):25-34
We investigated vascular access ports for feline blood donation. Eight cats were anesthetized for conventional blood collection by jugular venipuncture at the beginning and end of the study. In-between conventional collections, vascular access ports were used for collection with or without sedation every 6 to 8 wk for 6 mo. Ports remained functional except for one catheter breakage, but intermittent occlusions occurred. Systolic blood pressure was lower during conventional collection. Behavioral abnormalities occurred during 3 port collections. Packed red cells prepared from collected blood were stored at 4°C for 25 d and assessed for quality pre- and post-storage. With both collection methods, pH and glucose level declined, and potassium level, lactate dehydrogenase activity and osmotic fragility increased. There were no differences between methods in pre-storage albumin and HCO(3)(-) levels, and pre and post-storage hematocrit, lactate dehydrogenase activity, and glucose and potassium levels. Pre-storage pH and pCO(2) were higher with conventional collection, and pre- and post-storage osmotic fragility were greater with port collection. One port became infected, but all cultures of packed red cells were negative. Tissue inflammation was evident at port removal. In a second study of conventional collection in 6 cats, use of acepromazine in premedication did not exacerbate hypotension. The use of vascular access ports for feline blood donation is feasible, is associated with less hypotension, and may simplify donation, but red cell quality may decrease, and effects on donors must be considered. 相似文献
194.
Andi Flory Lisa McLennan Betsy Peet Marissa Kroll Deirdre Stuart Devon Brown Kathy Stuebner Brenda Phillips Brenda L. Coomber J. Paul Woods Mairin Miller Chelsea D. Tripp Amber Wolf-Ringwall Kristina M. Kruglyak Angela L. McCleary-Wheeler Ashley Phelps-Dunn Lilian K. Wong Chelsea D. Warren Gina Brandstetter Michelle C. Rosentel Lauren R. DiMarzio Allison L. O'Kell Todd A. Cohen Daniel S. Grosu Jason Chibuk Dana W. Y. Tsui Ilya Chorny Jill M. Rafalko 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):258-267
195.
Julia N. van Spijk Katrin Beckmann Meret Wehrli Eser Martina Boxler Martina Stirn Thea Rhyner Dana Kaelin Lanja Saleh Angelika Schoster 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(4):1525
BackgroundPolymyxin B (PolyB) is used to treat endotoxemia in horses; neurologic and nephrogenic adverse effects occur in humans.ObjectivesTo describe PolyB adverse effects in horses.AnimalsFive healthy horses (ataxia 0/5), 1 horse with cervical osteoarthritis (ataxia 1/5).MethodsProspective blinded randomized cross‐over trial; 3‐weeks wash out. Horses received PolyB (PolyB 6000 IU/kg IV, 7 doses q12h, n = 6) and PolyB/gentamicin (PolyB 6000 IU/kg IV, q12h 7 doses; gentamicin 10 mg/kg IV q24h 4 doses n = 4, or q12‐24 h 5 doses because of an additional erroneous dose, n = 2). Daily neurological examinations were video recorded, and ataxia graded by 3 observers. Urine status, urinary GGT/creatinine ratio, plasma creatinine, and urea were assessed every other day, EMG daily. Mixed model analysis was used to evaluate factors associated with ataxia grade and [PolyB].ResultsMedian ataxia score increased from 0/5 (range 0‐2/5) to 2/5 (range 1‐3/5) during administration and declined to 0.5/5 (range 0‐2/5) after cessation. Gentamicin co‐administration (P < .01, effect size: .8), number of PolyB doses (P < .001, effect size: .6), and time since last PolyB dose (P < .001, effect size: .5) had a significant effect on ataxia grades, while horse, day, [Genta], [PolyB], and [PolyB]CSF did not. Gentamicin co‐administration and [Genta] Cpeak had no effect on median [PolyB] Cpeak (4.67 and 4.89 μg/ml for PolyB and PolyB/gentamicin, respectively). Urinary GGT/creatinine ratio was elevated in 3/6 horses receiving PolyB/gentamicin. The EMG remained unchanged.Conclusions and Clinical ImportancePolyB caused transient ataxia, worsening with cumulative PolyB doses and gentamicin co‐administration. Nephrotoxicity of PolyB was only evident when gentamicin was co‐administered. 相似文献
196.
197.
The geological record of ocean acidification 总被引:1,自引:0,他引:1
Hönisch B Ridgwell A Schmidt DN Thomas E Gibbs SJ Sluijs A Zeebe R Kump L Martindale RC Greene SE Kiessling W Ries J Zachos JC Royer DL Barker S Marchitto TM Moyer R Pelejero C Ziveri P Foster GL Williams B 《Science (New York, N.Y.)》2012,335(6072):1058-1063
Ocean acidification may have severe consequences for marine ecosystems; however, assessing its future impact is difficult because laboratory experiments and field observations are limited by their reduced ecologic complexity and sample period, respectively. In contrast, the geological record contains long-term evidence for a variety of global environmental perturbations, including ocean acidification plus their associated biotic responses. We review events exhibiting evidence for elevated atmospheric CO(2), global warming, and ocean acidification over the past ~300 million years of Earth's history, some with contemporaneous extinction or evolutionary turnover among marine calcifiers. Although similarities exist, no past event perfectly parallels future projections in terms of disrupting the balance of ocean carbonate chemistry-a consequence of the unprecedented rapidity of CO(2) release currently taking place. 相似文献
198.
Kelley DS Karson JA Früh-Green GL Yoerger DR Shank TM Butterfield DA Hayes JM Schrenk MO Olson EJ Proskurowski G Jakuba M Bradley A Larson B Ludwig K Glickson D Buckman K Bradley AS Brazelton WJ Roe K Elend MJ Delacour A Bernasconi SM Lilley MD Baross JA Summons RE Sylva SP 《Science (New York, N.Y.)》2005,307(5714):1428-1434
The serpentinite-hosted Lost City hydrothermal field is a remarkable submarine ecosystem in which geological, chemical, and biological processes are intimately interlinked. Reactions between seawater and upper mantle peridotite produce methane- and hydrogen-rich fluids, with temperatures ranging from <40 degrees to 90 degrees C at pH 9 to 11, and carbonate chimneys 30 to 60 meters tall. A low diversity of microorganisms related to methane-cycling Archaea thrive in the warm porous interiors of the edifices. Macrofaunal communities show a degree of species diversity at least as high as that of black smoker vent sites along the Mid-Atlantic Ridge, but they lack the high biomasses of chemosynthetic organisms that are typical of volcanically driven systems. 相似文献
199.
[目的]探索和发掘优质抗麦长管蚜的种质资源及方法,为高效节本型和环境友好型的抗麦长管蚜小麦育种研究利用提供理论依据.[方法]以GIS的空间分布分析法和小麦种质资源抗蚜性鉴定为基础,从377份小麦种质资源中随机选取22个种质资源的13个主要性状,运用灰色系统理论对其进行综合分析.[结果]GIS的空间分布分析结果表明,麦长... 相似文献
200.
Hunt DE David LA Gevers D Preheim SP Alm EJ Polz MF 《Science (New York, N.Y.)》2008,320(5879):1081-1085
Identifying ecologically differentiated populations within complex microbial communities remains challenging, yet is critical for interpreting the evolution and ecology of microbes in the wild. Here we describe spatial and temporal resource partitioning among Vibrionaceae strains coexisting in coastal bacterioplankton. A quantitative model (AdaptML) establishes the evolutionary history of ecological differentiation, thus revealing populations specific for seasons and life-styles (combinations of free-living, particle, or zooplankton associations). These ecological population boundaries frequently occur at deep phylogenetic levels (consistent with named species); however, recent and perhaps ongoing adaptive radiation is evident in Vibrio splendidus, which comprises numerous ecologically distinct populations at different levels of phylogenetic differentiation. Thus, environmental specialization may be an important correlate or even trigger of speciation among sympatric microbes. 相似文献