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41.
Avian leukosis virus subgroup A (ALV‐A) is a retrovirus which infects egg‐type chickens and is the main pathogen of lymphoid leukosis (LL) and myeloid leukosis (ML). In order to greatly enhance the diagnosis and treatment of clinical avian leukemia, two monoclonal antibodies (MAbs) to ALV‐A were developed by fusion between SP2/0 and spleen cells from mice immunized with expressed ALV‐A env‐gp85 protein. Using immunofluorescence assay (IFA), two MAbs reacted with ALV‐A, but not with subgroups B and J of ALV. Western blot tests showed that molecular weight of ALV‐A envelope glycoprotein recognized by MAbs was about 53 kD. Isotyping test revealed that two MAbs (A5C1 and A4C8) were IgG1 isotypes. These MAbs can be used for diagnosis and epidemiology of ALV‐A. 相似文献
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Min Zhang Dan Zheng Zhimei Peng Yuting Zhu Rongrong Li Qiong Wu Yan Li Hongyi Li Weihua Xu Mao Zhang Qichun Hung Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131
In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers. 相似文献
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中国矮生刺黄瓜品系特性的研究初报 总被引:5,自引:0,他引:5
本文利用由中国蔓生刺黄瓜自然突变产生的矮生型黄瓜为材料,通过多低自交选育及性状观察,初步了解该矮生黄瓜植物学性状除具有自封顶、植株矮化性状外,与蔓生型黄瓜无明显差异。在生物学方面,该矮生黄瓜具有早熟、雌花节率高、结果集中、生长期短等特性。通过对中国矮生刺黄瓜与不同品种蔓生黄瓜杂交后代的观察、分析,初步认为黄瓜植株高度是由单基因体系和多基因体系共同作用决定的。 相似文献
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Effects of Moringa oleifera silage on milk yield,nutrient digestibility and serum biochemical indexes of lactating dairy cows 下载免费PDF全文
B. Zeng J. J. Sun T. Chen B. L. Sun Q. He X. Y. Chen Y. L. Zhang Q. Y. Xi 《Journal of animal physiology and animal nutrition》2018,102(1):75-81
This study investigated the effects of Moringa oleifera (MO) as a partial substitute of alfalfa hay on milk yield, nutrient apparent digestibility and serum biochemical indexes of dairy cows. MO was harvested at 120 days post‐seeding. Fresh MO was cut, mixed with chopped oat hay (425:575 on a DM basis), ensiled and stored for 60 days. Sixty healthy Holstein dairy cows were allocated to one of three groups: NM (no MO or control), LM (low MO; 25% alfalfa hay and 50% maize silage were replaced by MO silage) or HM (high MO; 50% alfalfa hay and 100% maize silage were replaced by MO silage). The feeding trial lasted 35 days. The LM and HM diets did not affect dry matter (DM) intake, milk yield or milk composition (lactose, milk fat, milk protein and somatic cell count). The apparent digestibility of DM and NDF was lower for HM group than NM group. Additionally, there were no significant differences in serum biochemical indexes between the LM and NM groups. The HM group had lower serum concentrations of total cholesterol, high‐density lipoprotein cholesterol, and low‐density lipoprotein cholesterol and higher serum concentrations of urea than the NM group. The partial replacement of alfalfa hay (≤50%) and maize silage with MO silage had no negative effects on milk yield, in vivo nutrient apparent digestibility or serum biochemical indexes of lactating cows. 相似文献
47.
新农村建设的核心在于建设现代农业。当前,临夏州正处于农业生产结构优化、农业提质增效的关键时期,建设社会主义新农村的任务十分艰巨。促进临夏现代农业农村经济又好又快发展,走具有地方特色现代农业,临夏州应把促进现代畜牧业发展摆在更加重要的位置,认真研究解决好如何加快畜牧业科技进步,转变畜牧业饲养方式,健全完善畜禽良种繁育体系,建立饲草饲料生产体系,培育和扶持市场竞争主体,建立有效的防疫体系,是发展现代畜牧业循环经济,促进新农村建设,助推脱贫攻坚的全局性突出问题。 相似文献
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为了筛选出防治萝卜蚜高效、低残留药剂,对30%乙酰甲胺磷乳油和40%氧乐果乳油防治萝卜蚜进行了药效对比及用量试验。田间试验结果表明,30%乙酰甲胺磷乳油对萝卜蚜具有理想的防治效果,表现出良好的速效性和持效性,使用30%乙酰甲胺磷乳油65~135mL/667m2,在蚜虫发生期内喷雾防治,施药第1d,防治效果就超过90%,药后第10d仍保持在90%以上,且对油菜安全。 相似文献
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戊唑醇分子印迹传感器制备及其农药残留检测的电学传感机理分析 总被引:1,自引:0,他引:1
以甲基丙烯酸为功能单体,戊唑醇(tebuconazole,TEB)为模板分子,乙二醇二甲基丙烯酸酯为交联剂,应用电化学聚合法制备了分子印迹电化学传感器(TEB-MIP/F-CNTs/GCE)。利用循环伏安法(cyclic voltammetry,CV)和交流阻抗法(electrochemical impedance spectroscopy,EIS)对新型传感器进行了表征,并用差分脉冲伏安法(differential pulse voltammetry,DPV)考察了新型传感器对戊唑醇的检测性能。结果表明:戊唑醇分子印迹电化学传感器具有一定特异性;传感器表观表面积比裸电极显著提高;新型传感器具有良好的印迹效果,相较于其他结构类似的三唑类化合物(如苯醚甲环唑),TEB-MIP/F-CNTs/GCE对戊唑醇表现出高效的识别能力,具有专一性和强选择性,且在C_(TEB)≤2.0×10~(-6)mol/L范围内传感器峰电流与C_(TEB)存在定量关系;数据模拟分析得到传感器的电学阻抗谱等效电路模型为R_1(CPE_1(R_2(CPE_2(R_3)))),计算各电极/传感器的等效电路中各元件参数,证明该模型能有效地模拟传感器检测戊唑醇的传感机理。该研究结果可为三唑类农药残留仿生免疫检测方法的建立提供技术支撑。 相似文献
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