The junction temperature simulation assessment model of IGBT module in three-phase inverters

Insulated gate bipolar transistor (IGBT) power module operating performance and reliability and its design of heat dissipation system in inverter are directly related to its operation junction temperature. Investigation of temperature of power device junc     

Y 系山定子 AP1同源基因的克隆及序列分析

以Y系山定子为试材研究AP1基因,试图为缩短果树童期提供理论依据。根据已登录的苹果AP1基因保守序列设计引物,以Y系山定子的基因组DNA为模板进行PCR扩增,获得1010 bp的基因片段;PCR产物纯化后,将其连接到pMD18-T载体上,连接产物转化大肠杆菌DH5α菌株感受态细胞,通过蓝白斑筛选、质粒长度和酶切鉴定,获得重组质粒并测序。测序结果表明,该序列有2个外显子、1个内含子及2个内含子的一部分,编码区共编码81个氨基酸;对氨基酸序列保守性分析发现,第13～81个氨基酸为MADS-box基因家族特有的K区域;其氨基酸序列在GenBank中同源性检索表明,Y系山定子与其他植物AP1同源基因的氨基酸序列同源性为67．9％～100％。研究结果为揭示Y系山定子早花现象的分子机制奠定了基础,对果树育种具有重要的理论价值和实际意义。     

作物空间诱变及其在遗传育种中的应用

空间诱变是进行作物种质资源创新及优良新品种选育的有效新途径。围绕作物空间诱变基础生物学研究的主要问题——诱变作用机理,阐述作物对空间环境条件下的感知、响应;针对作物空间诱变育种研究,总结其在地面的遗传变异规律及其种质资源创新中的应用。同时,对其当前存在的问题展开讨论,以期对今后我国空间诱变育种工作的有所启发。     

土下微膜覆盖与灌水管理对冬小麦水分利用与物质生产效果的影响

针对华北平原小麦生产水资源高耗与存量极度稀缺的问题,通过河北省两地大田试验,研究了全田土下微膜覆盖与不同灌水管理对冬小麦水分利用和物质生产效果的作用。结果表明,土下微膜覆盖条件下,冬小麦雨养或少量灌水消耗了占常规生产(对照) 2/3~3/4的耗水量,生产了不少于7500 kg hm^-2的籽粒产量;籽粒产量水分利用效率达到24.8~26.5 kg mm^-1 hm^-2,较对照提高28.3%~41.0%。与对照相比,生物产量水分利用效率高峰由抽穗扬花期提前至拔节期,且提高1.3~2.7倍,雨养或少量灌水还可有效提高小麦收获指数。土下微膜覆盖下的土壤贮水消耗速度变缓,2 m土体贮水量播种时不小于600 mm就可满足小麦的全生育期耗水,壤质土壤供水量为212.2 mm,黏质土壤供水量为230.0 mm。土下微膜覆盖下,雨养或扬花前少量灌水能够显著促进花后20 d内的光合速率,蒸腾速率减小同步显著提高光合水分利用效率,但在灌浆中期灌水则对提高光合速率及光合水分利用效率无意义。在华北平原,全田土下微膜覆盖雨养或适时少量灌水是大幅降低小麦耗水和提高水分利用效率,保证小麦产量的有效方法。     

Control of Sweeper and Anti-jamming Measures

Sweeper is a machine, which can clean up rubbish on the road surface with functions like drizzling, sweeping and sucking rubbish. Aiming at control of sweeper, the author introduced analyses on the theory of software and hardware of the system and their design using single chip computer (8752BH) as the core of control system. At the same time, the authors expatiated some anti-jamming measures, discussed the measures from the points of restraining electromagnetic noise, hardware and software anti-jamming to enhance the reliability of the control system.     

New Method to Study In-situ Stress of Rock Mass by AE and Structure Analysis of Rock Mass

In this paper, the authors adopt a method of structural analysis and stereography in combination with acoustic emission Kaiser Effect test in laboratory to find out the main direction of initial stresses. For overcoming the shortcomings of Kaiser effect by comparing it with traditional methods of measuring in-situ stresses, it is shown that this method is simpler, more economical and convenient for a large number of measurements and for studying the law of changes of regional in-situ stresses.     

Automatic Fingerprint Classification by GA-Based Neural Network

Fingerprint classification can provide an important indexing mechanism in a fingerprint database. An accurate and consistent classification can greatly reduce fingerprint matching time for large database. In the paper, by combining genetic algorithm and neural network is presented a fingerprint classification algorithm which is able to achieve an accurate classification. By inputting the global feature represented by directional image to three layer neural network trained by genetic algorithm, the fingerprints were classified into six categories: whorl, right loop, left loop, arch, double loop and undiscerning type successfully.     

Design Process Simulation Based On Information Dependency

A modeling method based on DSM is introduced. In circumstance of iterative task, the ways concerning about the input of data,the generation of stochastic variables and the set of the simulation clock are dipicted. While considering the learning curve of activities, the way is studied express the movable process of a single project and then list the corresponding Monte Carlo simulative block diagram .The program based of EXCEL VBA is also written. The result of a given simulative example shows that the method can simulate complex design activities and plan the project in terms of scientific basis.     

Value Innovation Method in Product Scheme Cost Design

Product design now has a significant influence on the profitability of a business. Enterprise simulation can not only overcome the limitations of current product scheme cost evaluation methods , but also support Product Scheme Cost design of Dynamic Enterprise. Product Scheme Cost Design basing on Value Innovation Method aim at value increase , and use Enterprise simulation as Product design tools. To evaluate different design options, a series of runs are performed in Enterprise simulation system for each design variant. In this way , Product design face on enterprise business. This paper focus on value chain,and use it in Product design, provide a new way for Product design of Dynamic Enterprise.     

三角帆蚌Rab3 cDNA全长克隆及其原核表达

根据三角帆蚌消减杂交cDNA文库获得的EST序列,运用cDNA末端快速扩增（rapid amplification of cDNA ends,RACE）技术获得三角帆蚌Rab3基因的全长cDNA序列（GenBank登录号为HQ190954）。序列全长1707 bp,开放阅读框666 bp,编码221个氨基酸,5’端非编码区为158 bp,3’端非编码区为883 bp。软件推测其编码蛋白相对分子量为24.92 KDa。NCBI Blastp进行氨基酸序列同源性分析表明,与居蟹皮海绵（Suberites domuncula）的Rab-3like（SdRab3）氨基酸相似性最高,为64%,构建进化树的结论也是先与居蟹皮海绵的Rab3-like（SdRab3）聚为一支。根据获得的Rab3基因cDNA全长序列,设计特异性引物扩增获得完整开放阅读框序列,并将已克隆到的三角帆蚌Rab3开放阅读框定向插入原核表达载体PQE-His质粒中,转化大肠杆菌BL21（DE3）。在30℃,5 h,1 mM IPTG的诱导条件下,Rab3蛋白得到高效表达,重组菌体经裂解和SDS-PAGE电泳分析,发现一条26 kDa左右特异的蛋白表达条带,其表达量约占菌体总蛋白30%。Western-blot检测表明,在健康组和病变三角帆蚌肝脏中,Rab3蛋白与β-actin的表达量比值分别为0.438和0.580。这一结果说明,Rab3基因在三角帆蚌经病原菌侵染后,表达量上调,且已有研究表明Rab3有通过调节中性粒细胞的胞吐作用来抵抗细菌侵入、防止机体感染的功能,从而初步推测三角帆蚌Rab3蛋白可能与三角帆蚌瘟病有关。