春丰50苦瓜的选育

以曼谷青皮、碧绿1号和钦州大肉苦瓜为育种材料,采用双父本混合花粉杂交和后代系谱选择育成优良自交系,再经配合力测定选出优良一代杂种春丰50。该品种生长势强,果实粗棒形,纵径26.3cm,横径6.45cm,皮色翠绿油亮,一般单果质量420g左右。平均每667m~2产量3 500kg。     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.     

2%氨基寡糖防治苹果花叶病

2001年8月应用2%氨基寡糖对苹果花叶病进行了田间防治试验 ,结果表明:2%氨基寡糖水剂稀释300、500倍液,对苹果花叶病防效达93.85%、89.42%。其防效比对照药剂20%病毒A 81.11%的防效高 ,比对照区增产21%、23% ,是防治苹果花叶病较理想的药剂     

细胞骨架解聚药物对小麦与叶锈菌互作诱发的细胞过敏性反应的影响

 小麦(Triticum aestivum)品种洛夫林10和叶锈菌小种366组成不亲和组合,小麦叶片发生过敏性坏死反应(HR)是小麦抵抗叶锈菌侵染的重要因素。在接种前给小麦叶片分别预注射微管解聚药物磺草硝(oryzalin)和微丝解聚药物细胞松弛素D (cytochalasin D,CD),结果表明2种药物注射使得寄主因叶锈菌侵染诱导的细胞过敏性坏死数目明显减少,并且注射药物的浓度越大,寄主细胞发生HR的数量越少。说明肌动蛋白和微管蛋白的聚合状态是诱发小麦叶片发生HR防卫反应所必需的,细胞骨架在小麦抵抗叶锈菌侵染过程中可能起着重要作用。     

大白菜软腐病菌游动性突变体在大白菜体内增殖扩展研究

 用培养皿滤纸吸附测定法和不伤根土壤拌菌处理及针刺接种法,测定了大白菜软腐病菌游动性突变体进入大白菜体内、并在其中侵染定殖和扩展的特性。结果表明,游动性丧失和增强的突变体都可以通过种子萌发和主动接触进入大白菜体内、并可以在体内有短期的繁殖,但菌量远低于野生菌。大白菜叶片接种实验说明,这两种突变体也都可以进行短距离扩展,但扩展距离和菌的繁殖量低于野生菌。     

植物杀虫剂苦皮藤素对柑橘潜叶蛾控制效果的研究

用植物性杀虫剂苦皮藤素Ⅳ(KPT乳油)有效成分30、15mg/kg防治柑橘潜叶蛾,采用离体叶片浸叶法处理5s,3d后,幼虫校正虫口减退率分别为89.63%、63.02%;枝梢浸液法处理5s,5d后,潜道伸长度校正抑制率分别为76.12%和41.51%;10d后,对秋梢的相对保叶效果分别为69.34%和52.29%。结果表明,采用KPT乳油有效成分30mg/kg对柑橘潜叶蛾有较好的控制作用,对柑橘秋梢有良好的保护效果。     

Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells

AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [³-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10^-6-10^-4 mmol/L) stimulated [³-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [³-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10^-6-10^-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl₂ for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.     

甜橙新品种‘兴国甜橙3-5’

 ‘兴国甜橙3-5 ’是从实生‘兴国甜橙’后代中选出的新品种。果皮橙黄至橙红色。果实近圆球形, 平均单果质量167. 7 g , 可溶性固形物含量13. 6% ~ 15. 1%, 总糖含量10. 3% ~ 11. 5%, 总酸含量0. 47%~ 0. 93%, 可食率79. 27%~ 80. 75%, 果汁率65. 18%~ 65. 96%, 11 月上旬成熟。     

典型光活化毒素α-三噻吩对棉铃虫和亚洲玉米螟Na~+-K~+-ATPase的抑制作用

以棉铃虫 H elicoverpa armigera和亚洲玉米螟 Ostrinia furnacalis为试虫 ,建立 Na+- K+-ATPase最佳反应系统 ,研究典型光活化毒素 α-三噻吩 (简称 α- T)对 Na+- K+- ATPase的影响。棉铃虫 Na+- K+- ATPase活力最佳测试条件为酶源蛋白浓度 6μg/ m L ,反应温度 35～ 4 0℃ ,反应时间6min;亚洲玉米螟则为酶源蛋白浓度 8μg/ m L,反应温度 35℃ ,反应时间 6min。近紫外光照 (30 0～4 0 0 nm )对棉铃虫和亚洲玉米螟离体 Na+- K+- ATPase活力基本没有影响 ,但对活体活力有很强的抑制作用。光照和无光照条件下 ,α- T对两种昆虫离体和活体 Na+- K+- ATPase活力均有不同程度的抑制作用。光照组 α- T对亚洲玉米螟 Na+- K+- ATPase的抑制率高于无光照组 ,且处理浓度或剂量越高 ,其抑制率越大 ;对棉铃虫 Na+- K+- ATPase抑制作用不显著