草原区沙地放牧草地合理利用途径

本文通过对草原区沙地放牧草地在畜牧业生产实践中出现大面积退化和沙化现象的分析 ,探讨了沙地放牧草地合理利用的途径 ,认为对于沙地放牧草地的保护 ,重点应放在草地的合理利用方面。通过以草定畜 ,合理调整载畜量 ;建立合理的利用制度 ;发展季节畜牧业 ;调整畜群结构 ,增加良种畜比例 ;以及改良天然草地 ,建立人工、半人工草地等措施 ,达到草原区沙地草地的可持续利用。     

春季休牧对草原和家畜生产力的影响

从4月上旬到6月下旬,在内蒙古锡林郭勒典型草原,进行春季休牧试验,对休牧和非休牧的草原进行生产力监测.结果表明:春季休牧可以有效地保护草原生态环境,经过两个月休牧后,草原植被状况明显改善;休牧期间采用低投入维持性饲养,可以显著降低饲养成本.并对休牧措施的生态效益、生产实践可行性和经济合理性进行综合讨论和评价.     

野生玫瑰组培快繁育苗技术的研究

以野生玫瑰当年生去叶带芽嫩茎为试验材料进行组培繁殖育苗技术研究。结果表明,野生玫瑰的外植体诱导丛生芽的最佳培养基配方为MS BA3.0 mg.L-1 NAA0.3 mg.L-1,最佳继代培养基为MS BA3.0 mg.L-1 NAA0.1 mg.L-1,增殖率达4.95倍;最佳生根培养基配方为1/2MS NAA0.1 mg.L-1,生根率达99%以上。     

小麦花后库源器官氮素、磷素营养物质积累动态分析

为探究小麦开花后不同部位氮素、磷素营养物质积累的动态规律,以中国春和贵紫1号2个小麦品种为材料,分别于花后5,15,25,35,45 d对小麦旗叶、茎鞘、颖壳穗轴和子粒中氨基酸、可溶性蛋白质、无机磷、植酸含量及单粒质量进行动态测定。结果表明,在子粒发育过程中,2个小麦品种库源器官氮素、磷素营养物质及单粒质量均有相似的变化趋势。除源器官的氨基酸和茎鞘的植酸外,其他氮素、磷素营养物质与子粒库中的5个营养性状均具有显著相关性。子粒发育中期库源器官氮素、磷素营养物质含量的差异远小于其前期与后期。结果还表明,小麦子粒发育中期(花后15～25 d)库源器官氮、磷代谢最为旺盛。建议此时期更应注重小麦氮、磷养分均衡,既满足子粒积累足够氮、磷营养,不致早衰,又同时注意防止其过量造成贪青晚熟,以保证小麦的优质高产。     

吉林省国有林场职工社会保险问题调查报告

通过对吉林省国有林场职工参加基本养老保险、基本医疗保险等五险情况的调查,指出其存在参保比率低、缴费标准低、欠费额度大、待遇不一致和地区间不平衡等问题。到2013年底,全省国有林场已参加社会保险单位累计欠缴基本养老保险保费15.2亿元,未参保职工若全部纳入保险范围,尚需补缴保费63.2亿元,数额十分巨大。建议国家尽快出台政策解决企事业单位参保待遇不同问题,同时省级财政和中央财政资金补助要保障最低线,对按社会平均工资60%下限标准缴费部分要全额保障。     

中国荞麦的育种现状与展望

1 中国荞麦的育种现状 1．1 荞麦的资源状况及分布 荞麦属蓼科(Polygonaceae)荞麦属(Fagypyrum Gaerth)。我国是世界荞麦的主产区之一，也是世界荞麦的起源中心和遗传多样性中心。全世界目前发现的荞麦共有15个种和2个变种，其中在我国就有10个种和2个变种。荞麦的栽培种有甜荞(Common Buckwheat)和苦荞(Tartary Buckwheat)两种，在我国均有种植。……     

Transfection of non-susceptible cells with Ovis aries recombinant lymphocyte function-associated antigen 1 renders susceptibility to Mannheimia haemolytica leukotoxin

Mannheimia haemolytica is an important etiological agent of pneumonia in domestic sheep (DS, Ovis aries). Leukotoxin (Lkt) produced by this organism is the principal virulence factor responsible for the acute inflammation and lung injury characteristic of M. haemolytica caused disease. Previously, we have shown that the leukocyte-specific integrins, beta(2) integrins, serve as the receptor for Lkt. Although it is certain that CD18, the beta subunit of beta(2) integrins, mediates Lkt-induced cytolysis of leukocytes, it is not clear whether CD18 of all three beta(2) integrins, LFA-1, Mac-1 and CR4, mediates Lkt-induced cytolysis of DS leukocytes. Since polymorphonuclear leukocytes, which express all three beta(2) integrins, are the leukocyte subset that is most susceptible to Lkt, we hypothesized that all three beta(2) integrins serve as the receptor for Lkt. The objective of this study was to determine whether DS LFA-1 serves as a receptor for M. haemolytica Lkt. We cloned the cDNA for DS CD11a, the alpha subunit of LFA-1, and co-transfected it along with the previously cloned cDNA for DS CD18, into a Lkt-non-suceptible cell line. Transfectants stably expressing DS LFA-1 were bound by Lkt. More importantly, Lkt lysed the DS LFA-1 transfectants in a concentration-dependent manner. Pre-incubation of Lkt with a Lkt-neutralizing monoclonal antibody (MAb), or pre-incubation of transfectants with MAbs specific for DS CD11a or CD18, inhibited Lkt-induced cytolysis of the transfectants. Exposure of LFA-1 transfectants to low concentrations of Lkt resulted in elevation of intracellular [Ca(2+)](i). Taken together, these results indicate that DS LFA-1 serves as a receptor for M. haemolytica Lkt.