全文获取类型
收费全文 | 7046篇 |
免费 | 463篇 |
国内免费 | 205篇 |
专业分类
林业 | 409篇 |
农学 | 486篇 |
基础科学 | 66篇 |
1353篇 | |
综合类 | 832篇 |
农作物 | 481篇 |
水产渔业 | 573篇 |
畜牧兽医 | 2732篇 |
园艺 | 160篇 |
植物保护 | 622篇 |
出版年
2023年 | 49篇 |
2022年 | 88篇 |
2021年 | 164篇 |
2020年 | 152篇 |
2019年 | 178篇 |
2018年 | 208篇 |
2017年 | 242篇 |
2016年 | 240篇 |
2015年 | 168篇 |
2014年 | 231篇 |
2013年 | 329篇 |
2012年 | 401篇 |
2011年 | 440篇 |
2010年 | 320篇 |
2009年 | 287篇 |
2008年 | 386篇 |
2007年 | 370篇 |
2006年 | 312篇 |
2005年 | 286篇 |
2004年 | 248篇 |
2003年 | 315篇 |
2002年 | 375篇 |
2001年 | 340篇 |
2000年 | 207篇 |
1999年 | 153篇 |
1998年 | 49篇 |
1997年 | 50篇 |
1996年 | 41篇 |
1995年 | 50篇 |
1994年 | 49篇 |
1993年 | 41篇 |
1992年 | 58篇 |
1991年 | 43篇 |
1990年 | 57篇 |
1989年 | 60篇 |
1988年 | 38篇 |
1987年 | 41篇 |
1986年 | 33篇 |
1985年 | 37篇 |
1984年 | 53篇 |
1983年 | 29篇 |
1982年 | 28篇 |
1980年 | 23篇 |
1979年 | 29篇 |
1978年 | 30篇 |
1977年 | 29篇 |
1975年 | 26篇 |
1974年 | 37篇 |
1972年 | 31篇 |
1971年 | 40篇 |
排序方式: 共有7714条查询结果,搜索用时 0 毫秒
61.
V De Cesaris S Grolli C Bresciani V Conti G Basini E Parmigiani E Bigliardi 《Reproduction in domestic animals》2017,52(2):235-242
In the last decade, progenitor cells isolated from dissociated endometrial tissue have been the subject of many studies in several animal species. Recently, endometrial cells showing characteristics of mesenchymal stem cells (MSC) have been demonstrated in human, pig and cow uterine tissue samples. The aim of this study was the isolation and characterization of stromal cells from the endometrium of healthy bitches, a tissue that after elective surgery is routinely discarded. Multipotent stromal cells could be isolated from all bitches enrolled in the study (n = 7). The multipotency of cells was demonstrated by their capacity to differentiate into adipocytic, osteocytic and chondrocytic lineages. Clonogenicity and cell proliferation ability were also tested. Furthermore, gene expression analysis by RT‐PCR was used to compare the expression of a set of genes (CD44, CD29, CD34, CD45, CD90, CD13, CD133, CD73, CD31 CD105, Oct4) with adipose tissue‐derived MSC. Stromal cells isolated from uterine endometrium showed similar morphology, ability of subculture and plasticity, and also expressed a panel of genes comparable with adipose tissue‐derived MSC. These data suggest that endometrial stromal cells fulfil the basic criteria proposed by the “Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy” for the identification of mesenchymal stem cells. Although endometrial mesenchymal stem cells (EnMSC) showed a lower replicative ability in comparison with adipose tissue‐derived MSC, they could be considered a cell therapeutic agent alternative to adipose tissue or bone marrow‐derived MSC in dog. 相似文献
62.
鸡毒霉形体HS株pMGA多基因族序列分析 总被引:2,自引:1,他引:2
用限制性核酸内切酶对鸡毒霉形体(MG)HS株基因文库中经初步估测可能含有的4个鸡毒霉形体粘附蛋白(protein of Mycoplasma gallisepticum adhesin,pMGA)基因的MgW17重组质粒进行了不同组合的酶消化,根据消化片段的大小及酶切位点绘制了7.5kb外源片段的物理图谱;然后根据所得的物理图谱,选用Pst I和EcoR I将MgW17外源片段酶解成1.3、2.0、4.2 kb 3个部分,将它们分别亚克隆到SK(十)质粒上,得到了3个亚克隆子SGp100、SGp200、SGp300.使用核酸外切酶Ⅲ缺失法构建缺失子系列,经序列分析后得到MgW17外源片段的全部序列.序列全长7 434 bp,中间含有2个完整的pMGA基因和另2个不完整的pMGA基因的首部和尾部,分别将它们顺次命名为H-pMGA1.1、H-pMGA1.2、H-pMGA1.3和H pMGA1.4.2个完整的pMGA基因的阅读框长度分别为1 967 bp(1.2)和2039 bp(1.3);H-pMGA1.1首部不完整的阅读框的长度为720 bp,尾部不完整的H-pMGA1.4的长度为1 752 bp.将H-pMGA基因与已报道的MG.S6株、F株的pMGA基因进行了比较,探讨了pMGA基因在不同MG菌株中的相似性、基因启动子结构的差异、间隔区内GAA重复序列对转录调控的影响等. 相似文献
63.
用从木瓜酶修饰的猪红细胞提取的 CD5 8分子 ,协同 ND 疫苗免疫 8日龄雏鸡 (分为试验 A组、对照 B组 ) ;用从正常猪红细胞提取的 CD5 8分子 ,协同 ND 疫苗免疫 18日龄雏鸡 (分为试验 C组、对照 D组 ) ,以β-微量法测定ND抗体效价 ,以 α-醋酸萘酯酶染色法检测鸡外周血 T细胞的变化 ,并分别用 NDV F4 8E9强毒株在免疫后 2 4 d攻击 A组和 B组鸡、39d攻击 C组和 D组鸡。结果表明 ,免疫前 ,8日龄雏鸡的 ND抗体效价为 2 5.8,18日龄雏鸡的 ND抗体均为阴性 ;免疫后 ,与对照 D组比较 ,试验 C组的 ND抗体提前 4 d达到 2 4 .8,并且试验 C组 ND抗体效价显著高于对照 D组 ( P<0 .0 1) ,保护期延长 ;试验 A组雏鸡体内 ND抗体变化和对照 B组相似 ,但试验 A组雏鸡外周血 T细胞数量显著高于对照 B组 ( P<0 .0 1或 P<0 .0 5 )。攻毒试验结果显示 ,试验 A组鸡死亡率为 33% ,对照 B组鸡死亡率为6 3% ;试验 C组鸡死亡率为 17% ,对照 D组鸡死亡率为 5 3%。本研究结果表明 ,猪红细胞 CD5 8分子具有较好的疫苗佐剂效应 相似文献
64.
65.
Felipe Nogueira Soares Maria Emilia Franco Oliveira Luciana Cristina Padilha-Nakaghi Luís Guilherme de Oliveira Marcus Antônio Rossi Feliciano Felipe Brener Bezerra de Oliveira Pedro Paulo Maia Teixeira Wilter Ricardo Russiano Vicente Cristian Faturi Luiz Fernando de Souza Rodrigues 《Tropical animal health and production》2015,47(8):1465-1471
66.
Enantioselective pharmacokinetics of ketoprofen in calves after intramuscular administration of a racemic mixture 下载免费PDF全文
E. Plessers A. Watteyn H. Wyns B. Pardon S. De Baere P. De Backer S. Croubels 《Journal of veterinary pharmacology and therapeutics》2015,38(4):410-413
The pharmacokinetic properties of ketoprofen were determined in 4‐week‐old calves after intramuscular (i.m.) injection of a racemic mixture at a dose of 3 mg/kg body weight. Due to possible enantioselective disposition kinetics and chiral inversion, the plasma concentrations of the R(?) and S(+) enantiomer were quantified separately, using a stereospecific HPLC‐UV assay. A distinct predominance of the S(+) enantiomer was observed, as well as significantly different pharmacokinetic parameters between R(?) and S(+) ketoprofen. More in specific, a greater value for the mean area under the plasma concentration–time curve (AUC0→∞) (46.92 ± 7.75 and 11.13 ± 2.18 μg·h/mL for the S(+) and R(?) enantiomer, respectively), a lower apparent clearance (Cl/F) (32.8 ± 5.7 and 139.0 ± 25.1 mL/h·kg for the S(+) and R(?) enantiomer, respectively) and a lower apparent volume of distribution (Vd/F) (139 ± 14.7 and 496 ± 139.4 mL/kg for the S(+) and R(?) enantiomer, respectively) were calculated for the S(+) enantiomer, indicating enantioselective pharmacokinetics for ketoprofen in calves following i.m. administration. 相似文献
67.
68.
Martin Bonamy Sabrina Kluska Elisa Peripolli Marcos Vinícius Antunes de Lemos Sabrina Thaise Amorim Roberto Jose Vaca Raysildo Barbosa Lbo Letícia Mendes de Castro Carina Ubirajara de Faria Fabio Borba Ferrari Fernando Baldi 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2019,136(1):15-22
The aim of this study was to estimate genetic parameters for different precocious calving criteria and their relationship with reproductive, growth, carcass and feed efficiency in Nellore cattle using the single‐step genomic BLUP. The reproductive traits used were probability of precocious calving (PPC) at 24 (PPC24), 26 (PPC26), 28 (PPC28) and 30 (PPC30) months of age, stayability (STAY) and scrotal circumference at 455 days of age (SC455). Growth traits such as weights at 240 (W240) and 455 (W455) days of age and adult weight (AW) were used. Rib eye area (REA), subcutaneous fat thickness (SFT), rump fat thickness (RFT) and residual feed intake (RFI) were included in the analyses. The estimation of genetic parameters was performed using a bi‐trait threshold model including genomic information in a single‐step approach. Heritability for PPC traits was moderate to high (0.29–0.56) with highest estimates for PPC24 (0.56) and PPC26 (0.50). Genetic correlation estimates between PPC and STAY weakened as a function of calving age. Correlation with SC455, growth and carcass traits were low (0.25–0.31; ?0.22 to 0.04; ?0.09 to 0.18, respectively), the same occurs with RFI (?0.09 to 0.08), this suggests independence between female sexual precocity and feed efficiency traits. The results of this study encourage the use of PPC traits in Nellore cattle because the selection for such trait would not have a negative impact on reproductive, growth, carcass and feed efficiency indicator traits. Stayability for sexual precocious heifers (PPC24 and PPC26) must be redefined to avoid incorrectly phenotype assignment. 相似文献
69.
70.
A serologic survey in unvaccinated broiler parent and broiler progeny flocks demonstrated seroconversion against chicken infecrious anemia virus (CIAV) in all parent flocks before or around point of lay and in 38% of the broiler flocks examined at slaughter age. The presence of CIAV antibodies at slaughter of broilers was positively correlated with slaughterhouse condemnation rates. Results indicate that CIAV infections are highly prevalent in both broiler parent and broiler flocks and that CIAV infections in broilers are associated with increased slaughterhouse condemnation. 相似文献