Comparative analysis of some selected macronutrients of soil in orange orchard and degraded forests in Chittagong Hill Tracts,Bangladesh

Status of organic carbon （C）, total nitrogen （N）, available potassium （K）, calcium （Ca） and phosphorus （P） in three different depths （0-5 cm, 5-15 cm and 15-30 cm） on two hill slopes of 35% and 55% in orange orchard cultivated by the Mro tribe of Chittagong Hill. Tracts （CHTs） were evaluated and compared with those in degraded bush forests, through digging three profiles in each land use. The content of all the five nutrients was found to be higher in the soil of orange orchard than in the soil of forest. But the variation was not consistent for both the slopes. The content varied depth wise also, having the highest value in surface soil in case of both the land uses on both the slopes. A mean available K content was significantly higher in orange orchard than in forest on 55% slope, while it was lower on 35% slope. Surface soil contained the nutrients of K and Ca with the amount of 0.2905-mg·g^-1 soil and 3.025-mg·g^-1 soil respectively in the orchard, while 0.1934-mg·g^-1 soil and 1.6083-mg·g^-1 soil were respectively in the forest. Organic carbon and total nitrogen were found more or less similar in surface soil on both the land uses showing a slight difference. Available P was found only in orange orchard, and in forest it was too little in amount to detect by the spectrophotometer. The degraded forests were poor in nutrient content due to high rate of soil erosion, which would be possible to be improved by bringing it under tree cover as proved by the adaptation of orange orchard there.     

Use of four types of litter for rearing broilers

1. One hundred and forty four Shaver broiler chicks (Starbro 15) were reared from 4 d to 56 d of age on 4 types of litter: sawdust, paddy straw, sand and rice husk each spread to a depth of 75 mm.

2. Birds reared on rice husk litter showed the greatest food consumption, greatest weight gain, best food conversion efficiency and scored highest in production number.

3. Survivability was also highest with rice husk group (94.4%) but differences in this variable were not thought to be attributable to types of litter.

4. Breast blisters were found only in birds reared on sand (8.3%).

5. It was concluded that rice husk is a suitable litter for rearing broilers on the floor, particularly in paddy‐growing countries.     

Pencycuron dissipation in soil: effect of application rate and soil conditions

Dissipation of the fungicide pencycuron was examined under controlled laboratory conditions in clay loam soils from rice cultivated fields of alluvial soil (Typic udifluvent) and coastal saline soil (Typic endoaquept) at field rate, twice the field rate and 10 times the field rate with and without decomposed cow manure maintained at 60% of maximum water-holding capacity (WHC) and waterlogged soil condition. The half-life values depended on the initial concentrations of pencycuron. Pencycuron, in general, degraded fastest in coastal saline soil and in soil amended with decomposed cow manure at 60% of maximum WHC of soil.     

Use of rice husk litter at different depths for broiler chicks during summer

1. Four groups of 36 one‐day‐old broiler chicks were reared for 8 weeks during summer on rice husk litter spread to depths of 20, 30, 40 or 50 mm.

2. The depth of the litter did not significantly affect live weight gain, food consumption, food conversion ratio, liveability or production number.

3. It was concluded that rice husks can be used as litter at depths of between 20 and 50 mm during summer to raise broilers without affecting performance.     

Effects of dietary supplementation of spices on forage degradability,ruminal fermentation,in vivo digestibility,growth performance and nitrogen balance in Black Bengal goat

The objective of the study was to evaluate the effects of spices on forage utilization and nitrogen (N) emission using in vitro and in vivo approach. A 6 × 5 factorial triplicate arrangement was used to assess the in vitro degradability of rice straw with control (without spices) and individual (40 mg/g rice straw) spices (cumin, coriander, clove, black cumin, turmeric) at five different incubation times. In vitro dry matter (DM) and organic matter (OM) degradability of rice straw were highest in presence of spices except for clove. Clove significantly reduced the total volatile fatty acids concentration, molar proportion of acetate and propionate ratio, but increased propionate production. Acetate and butyrate production were not affected by treatments. The ammonia‐nitrogen concentration was lowest for clove and turmeric compared to other spices. Rumen pH was unchanged but gradually decreased over the incubation period. For in vivo study, 12 bucks with average live weight 7.65 ± 0.19 kg were assigned to a completely randomized design with three treatments and four replicates for a 28‐day period. Bucks were fed a total mixed ration without (0 g/kg DM) or with (2.5, 7.5 g/kg DM) clove supplementation. DM intake, body weight and apparent total tract digestibility of crude protein (CP) and acid detergent fibre (ADF) were not affected by increasing dietary doses of clove but tended (p = .09) to increase DM and OM digestibility. The urinary N and urine urea N concentration of bucks decreased linearly with incremental doses of clove diet. In contrary, clove supplementation had positive effects on plasma urea N and retained N in bucks. We concluded that though some spices had positive effects on ruminal digestion and fermentation, the dosage level assessed in the current study (up to 7.5 g/kg DM clove) can be promoted as an effective dietary approach to mitigate N losses in bucks.     

Assessing the effect of crop residue removal on soil organic carbon storage and microbial activity in a no‐till cropping system

Changes in agricultural management strategies have received much attention in recent years with a view to increasing or maintaining the amount of carbon (C) sequestered as soil organic C (SOC). In many parts of the world, minimum or no‐till management has been promoted as a means of improving soil quality, reducing losses of erosion and potentially increasing SOC stocks. However, no‐till systems can become problematic and potentially disease‐prone, especially due to high crop residue loadings. Consequently, residue removal either by harvesting or burning off may be employed to reduce these pressures. Here, we examined the effect of crop residue removal on C storage in soil that had been under no‐till management for 20 yr. We predicted improved physical properties (i.e. lower bulk density) and greater microbial activity under the residue retention soils due to greater readily available C and nutrients derived from crop residues. In contrast, we predicted relative reductions in SOC in the no residue soils due to a lack of available residue‐derived C for microbial use. Residue removal caused a relative C loss from the soil, which was related to C input, amount of nutrient availability and microbial activity. We demonstrate the importance of maintaining crop residue cover in no‐till cropping systems for soil function and highlight the potentially deleterious effects of changing management strategy to increased residue harvesting or removal by burning.     

High frequency regeneration and heritable somaclonal variation in Brassica juncea

Summary Multiple shoot formation in cotyledonary callus of Indian mustard (Brassica juncea cv. Prakash) was induced on modified MS media supplemented with high cytokinin (kinetin or zeatin) and low IAA concentrations. Complete plants were obtained on prolonged incubation of shoots on the same medium. 6-Benzyladenine alone or in combination with IAA or NAA did not support plantlet regeneration. A total of 71 plants were transferred to greenhouse. The seed, however, could be collected from 37 plants only. The seed was sown in the field to evaluate the material for somaclonal variation in R₁ generation. Data were recorded for yield, plant height, number of primary branches, siliqua number, 1,000 seed weight and oil content. Somaclonal lines showed tremendous amount of variation for all the characters studied. A number of plants in this generation showed significantly higher yield and/or other improved agriculturally important characteristics as compared to the control. A line with dwarf plant type was also identified. A number of plants were selected from this generation and carried forward to R₂ generation. Most of these lines bred true in R₂ generation. The material seems to be very promising for future breeding programmes.     

RAPD markers linked to resistance to downy mildew disease in soybean

To determine and utilize RAPD markers linked to resistance to downymildew incited by Peronospora manshurica in soybean, a resistantcultivar `AGS129' was crossed to a susceptible cultivar `Nakhon Sawan 1'(NS1). F₂ and BC₁ populations were advanced from the F₁ and evaluatedfor resistance to the disease. ²-test demonstrated that the resistancewas controlled by a single dominant gene (Rpmx). Near-isogenic lines(NILs) and bulked segregant analysis (BSA) were used to identify RAPDmarkers linked to the gene. Six DNA bulks namely F₅(R), F₅(S),BC₆F₃(R), BC₆F₃(S), F₂(R) and F₂(S) were set up by pooling equalamount of DNA from 8 randomly selected plants of each disease responsetype. A total of 180 random sequence decamer oligonucleotide primerswere used for RAPD analysis. Primer OPH-02 (5 TCGGACGTGA 3 andOPP-10 (5 TCCCGCCTAC 3) generated OPH-02₁₂₅₀ and OPP-10₈₃₁fragments in donor parent and resistant bulks, but not in the recurrentparent and susceptible ones. Co-segregation analysis using 102 segregatingF₂ progenies confirmed that both markers were linked to the Rpmxgene controlling downy mildew disease resistance with a genetic distance of4.9 cm and 23.1 cm, respectively. Marker OPH-02₁₂₅₀ was presentin 13 of 16 resistant soybean cultivars and absent in susceptible cultivars,thus confirming a potential for MAS outside the mapping population.     

Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.)

Twenty two RAPD and 22 ISSR markers were evaluated for their potential use in determination of genetic relationships in chickpea (Cicer arietinum L.) cultivars and breeding lines. We were able to identify six chickpea cultivars/breeding lines by cultivar-specific markers. All of the cultivars tested displayed a different phenotype generated either by the RAPD or ISSR primers. Though ISSR primers generated less markers than RAPD primers, the ISSR primers produced higher levels of polymorphism (% of polymorphic markers per primer) than RAPD primers. A high level of within cultivar homogeneity was observed in chickpea. Cultivars/breeding lines originating from a common genetic background showed closer genetic relationship. Chickpea lines with similar seed type(kabuli or desi) had a tendency to cluster together. This revised version was published online in August 2006 with corrections to the Cover Date.