全文获取类型
收费全文 | 28546篇 |
免费 | 671篇 |
国内免费 | 1324篇 |
专业分类
林业 | 4878篇 |
农学 | 2911篇 |
基础科学 | 1117篇 |
4441篇 | |
综合类 | 5021篇 |
农作物 | 2929篇 |
水产渔业 | 2282篇 |
畜牧兽医 | 2668篇 |
园艺 | 1671篇 |
植物保护 | 2623篇 |
出版年
2024年 | 64篇 |
2023年 | 162篇 |
2022年 | 486篇 |
2021年 | 542篇 |
2020年 | 523篇 |
2019年 | 511篇 |
2018年 | 3044篇 |
2017年 | 3175篇 |
2016年 | 1611篇 |
2015年 | 656篇 |
2014年 | 574篇 |
2013年 | 805篇 |
2012年 | 1758篇 |
2011年 | 3045篇 |
2010年 | 2990篇 |
2009年 | 2039篇 |
2008年 | 2037篇 |
2007年 | 2226篇 |
2006年 | 652篇 |
2005年 | 594篇 |
2004年 | 377篇 |
2003年 | 346篇 |
2002年 | 261篇 |
2001年 | 218篇 |
2000年 | 240篇 |
1999年 | 211篇 |
1998年 | 183篇 |
1997年 | 119篇 |
1996年 | 148篇 |
1995年 | 149篇 |
1994年 | 141篇 |
1993年 | 126篇 |
1992年 | 113篇 |
1991年 | 91篇 |
1990年 | 66篇 |
1989年 | 74篇 |
1988年 | 67篇 |
1987年 | 31篇 |
1986年 | 16篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 13篇 |
1977年 | 4篇 |
1974年 | 2篇 |
1970年 | 3篇 |
1968年 | 4篇 |
1964年 | 3篇 |
1963年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
高梁不育系Tx3197A减数分裂中染色体行为 总被引:3,自引:0,他引:3
以Tx3197B和晋粱5号为对照,对Tx3197A进行了观察。结果发现,减数分裂前期Ⅰ和中期Ⅰ无明显异常。自后期Ⅰ开始,染色体行为出现了多类型的异常现象,导致减数分裂不能正常进行,无法形成花粉粒,从而产生雄性不育。 相似文献
32.
K-S. Dai C-S. Liang Y. T. Ch'iu P-C. Yang I. C. Cheng 《Veterinary research communications》1995,19(2):115-125
The purpose of this study was to determine whether myocardial adenosine triphosphatase (ATPase) activities were reduced in pigs with naturally occurring hypertrophic cardiomyopathy (HCM). The selection of hearts for the HCM and the normal control groups depended on histological examination. Specific ATPase activity and 5-nucleotidase activity were measured in left ventricular myocardium obtained from HCM (n=7) and normal control (n=7) animals. The histological features of HCM included marked disorientation of muscle cells, thickening of the intramural coronary arterial wall with a narrowed lumen, endocardial fibrosis and myocardial fibrosis. The HCM group showed significant increases in both heart weight (32%) and heart weight to body weight ratio (46%). The total ATPase activity in crude homogenates from the HCM group was significantly decreased by 16%. Azide-sensitive ATPase (mitochondrial ATPase) activity, ouabain-sensitive ATPase (Na+,K+-ATPase) activity, basal Mg2+-ATPase activity and Ca2+-ATPase activity were all significantly decreased by 18%, 30%, 20% and 50%, respectively. In contrast, no significant decrease was found in the mean values for 5-nucleotidase activity. These results suggest that myocardial ATPase activities are suppressed in pigs with naturally occurring HCMAbbreviations ATP
adenosine triphosphate
- gww
grams wet weight
- HCM
hypertrophic cardiomyopathy
- Pi
inorganic phosphate 相似文献
33.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
34.
35.
36.
几种植物提取物和药剂对桃蚜和萝卜蚜生长发育的影响 总被引:9,自引:1,他引:9
研究了苍耳提取物和合果芋(白蝴蝶)提取物以及常用天然药剂机油乳剂和 0. 3 %印楝素乳油对桃蚜和萝卜蚜实验种群的影响,两种提取物和药剂对桃蚜一龄若蚜的影响依次为: 0 3 % 印楝素乳油 (2 000×) >苍耳提取物(DW 0 04g·mL-1 ) >机油乳剂(400 ×) >白蝴蝶提取物(DW 0 04g·mL-1 ),对萝卜蚜一龄若蚜的影响依次为: 0. 3 %印楝素乳油(2 000×) >机油乳剂 ( 400×) >苍耳提取物 (DW 0. 04g·mL-1 ) >白蝴蝶提取物(DW 0. 04g·mL-1 );苍耳提取物对萝卜蚜实验种群有明显干扰控制作用,处理后萝卜蚜下代的种群增长量仅为对照的 24. 9 %;此外,苍耳提取物可延缓桃蚜和萝卜蚜的生长发育进程,使发育历期延长,对桃蚜的作用效果比对萝卜蚜的作用效果强;白蝴蝶提取物对萝卜蚜的实验种群也有一定的干扰作用,并可延长桃蚜的发育历期。 相似文献
37.
台湾青枣不同品种花粉萌发和生活力测定 总被引:12,自引:0,他引:12
以5个台湾青枣品种盛花期花粉为试材,应用正交试验测定不同品种花粉萌发率及适宜的萌发条件,并用4种不同方法测定花粉生活力。结果表明:(1)不同品种的花粉萌发率存在显著差异,其中大世界的花粉萌发率最高。(2)硼酸浓度、培养温度等因素对花粉萌发率有极显著影响。最适培养基为含琼脂1%、蔗糖15%、硼酸0.02%的组合,适宜温度为30—32℃。(3)花粉生活力的测定中,离体萌发法是最佳选择,I—KI染色法、醋酸洋红染色法和TTC染色法不适宜用作台湾青枣花粉生活力的测定。 相似文献
38.
39.
2001~2004年在齐齐哈尔市龙沙公园对黑鹳F2的5只雏鸟的人工孵化温度和湿度进行记录。并对人工育雏成活的雏鸟的一系列生长指标如:体重、体长、喙长、翅长、跗跖长和尾长等进行测量,探讨黑鹳体长、体重等生长指标生长变化规律。结果表明:黑鹳卵的前中期(1~29d)孵化温度为37.7℃,湿度为55%,后期(29~31d)出雏温度为37℃,相对湿度65%;育雏箱的温度前7d为37℃,以后以每天0.5℃幅度逐渐降低,箱内湿度保持在55%,20日龄后可在室温下饲养;黑鹳F2雏鸟各生长指标的增长呈Logistic曲线。 相似文献
40.
Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献