Seismic Behavior of Asymmetric Building under Pushover Analysis

Pushover analysis is a tool to study the inelastic behavior of structures under strong seismic action. The 3D-pushover analysis was performed to analyze the inelastic lateral-torsional coupled response of multistory asymmetric structures. The symmetric frame and frame-wall systems were studied in this paper, and the capability curve of the top displacement and base shear,the story drift and the position of rotation center were compared with reference to corresponding symmetric structures. The characters of inelastic response of multistory asymmetric structures were found. The conclusion provides reference for performance-based seismic design of asymmetric structures.     

棉田膜内与膜间土壤溶液含盐量的变化

用定期取表层土样测定其电导率和在不同深度埋设盐分传感器探头的方法 ,在作物生长季节内 ,监测了棉田膜内和膜间不同深度的土壤溶液含盐量变化。结果为 :1棉田膜内与膜间表层土壤和 1 0 cm、30 cm深土壤溶液含盐量变化较大 ,且规律相同。未灌水时上升 ,灌水后迅速下降。膜间未灌水期间的含盐量上升幅度大于膜内 ,灌水对膜内和膜间表土洗盐、1 0 cm、30 cm深土壤溶液含盐量的降低作用没有差别。降雨可引起膜内和膜间 1 0 cm深土壤溶液电导率的变化 ,这个影响也是膜间大于膜内。 2 60 cm深的土壤溶液不存在明显的盐分累积现象 ,灌水可使这里的土壤溶液含盐量缓慢降低 ,膜间的降低过程快于膜内     

低温驯化对菜豆内源多胺含量及其合成分解基因表达的影响

以7个菜豆品种为试材,采用对冷害指数以及相对电解质渗透率进行测定的方式,筛选出耐低温型菜豆品种和低温敏感型菜豆品种,并对其低温驯化过程中内源多胺含量进行测定,研究了腐胺、亚精胺和精胺在响应低温驯化过程中含量的变化,以及对多胺合成分解酶基因表达量的影响,以期为今后菜豆外施多胺缓解低温胁迫提供参考依据.结果 表明:在低温驯化过程中2个品种叶片中腐胺和亚精胺的含量有明显增加,而精胺含量变化规律不明显.在低温驯化过程多胺合成酶基因ADC表达量上升,ODC表达量变化不明显,SAMDC表达量升高,SPDS和SPMS在2个品种中表现不一致;多胺分解酶基因DAO和PAO表达量上升,SMO在2个品种中表现不一致.综上所述,腐胺和亚精胺在植物体内的积累可能与提高菜豆苗期耐低温性有较大关系,并且它们的积累可能与ADC、SAMDS、DAO和PAO的表达水平有关.     

发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响

为分析发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响,本研究将60只C57BL/6小鼠随机分为5组：正常对照组（CON）、模型组（MD）、易善复组（YG）、低浓度发酵乳干预组（LFG）和高浓度发酵乳干预组（HFG）。肥胖模型造模成功后进行为期6周的灌胃,试验结束测量小鼠的空腹体重和身长,计算LEE’S指数,采血检测生化指标,采集肝脏组织观察病理变化;采集小鼠盲肠内容物进行肠道菌群测序。结果表明：1）与CON相比,MD小鼠体重、LEE’S指数、ALT和AST均极显著提升（P<0.01）,表明肥胖模型建立成功。2）与MD相比,发酵乳干预组小鼠体重、LEE’S指数、ALT和TC均极显著下降（P<0.01）,其中AST和TG显著下降（P<0.05）,同时发酵乳和易善复组小鼠肝组织脂肪变性有明显好转,基本没有明显的脂肪空泡。表明发酵乳可以抑制肥胖小鼠体重的增加,改善肥胖小鼠的肝脂代谢功能。3）与MD相比干预组小鼠肠道菌群的多样性水平有一定程度的恢复,尤其是厚壁菌门和拟杆菌门。表明发酵乳对于小鼠肠道菌群多样性的恢复有一定的作用。综上,本研究阐明了发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响,为功能型发酵乳的开发提供理论依据。     

日本环境保全型耕地农业系统对中国耕地保护的启示

为解决中国农业长期粗放经营问题,研究中国耕地与生态文明协同保护体系,采用文献研究法和归纳演绎法,分析东亚地区代表国家环境保全型农业下的耕地农业协同体系。研究表明,日本的耕地农业环境保护支付制度、生态补偿市场认证体系、耕地农业法律制度保障,是建立在生态补偿原则上,经过实践检验行之有效的,能够提升市场参与度及农民自主性投入的耕地生态协同保护措施。中国在推广耕地合理利用实践方面做出了不懈努力,但是在建立耕地生态协同保护的休耕制度、因地制宜建立生态补偿体系、加快推进耕地生态协同保护专项基金的设立等方面还需进一步坚持和完善。     

重组酶聚合酶等温扩增技术在动物源性成分鉴定中的应用

在打击肉制品掺假走私、维护市场秩序、保护野生动物资源等方面,对动物源性成分鉴定技术的特异性、灵敏性、重复性提出了很高的要求。本文阐述了动物源性成分的检测鉴定技术,有多样性和局限性的特点。归纳了重组酶聚合酶恒等温扩增技术原理与应用,解决了PCR技术在仪器上的局限性,优化了早期恒温扩增技术的缺点。指出重组酶聚合酶恒等温扩增技术为动物源性成分的快速准确鉴定提供了坚实的技术基础,并在动物疫病诊断方面也有很好的应用前景。     

农业科研院所视角下新兴交叉学科发展的思考

新兴交叉学科不仅是重大科学成果产生的源泉,也是解决当今社会发展过程中出现的复杂问题的重要途径.文章梳理了部分农业科研院所在新兴交叉研究领域的动向,从省级农业科研院所角度,深入探讨了新兴交叉学科的发展特点和面临形势,从产业应用目标定位、对外交流合作、复合型人才培养引进、学科建设投入、学科发展规律研究和体制机制创新等方面提出了促进农业科研院所新兴交叉学科发展的建议.     

施氮处理对不同筋型小麦产量和品质的影响

为探究施氮处理对不同筋型小麦的植株性状、籽粒产量和品质的影响,采用盆栽试验,选用强筋小麦品种中麦578（A1）和中麦5051（A2）、弱筋小麦品种扬麦15（A3）和扬麦24（A4）为供试品种,在施氮量相同的条件下进行底施（B1）和追施（B2）处理。结果表明：在其他栽培措施相同条件下,强筋小麦穗长、总小穗数、千粒重和籽粒产量均优于弱筋小麦,其中A1籽粒产量分别比A2、A3和A4高0.44%、49.81%和15.27%;施氮处理中B2的株高、穗长、穗粒数、总小穗数和籽粒产量均高于B1;不同处理组合中,强筋小麦品种A1B2的植株和产量性状优于其他处理;强筋小麦品种的籽粒蛋白质含量和蛋白质产量均高于弱筋小麦品种,且具有极显著差异（P<0.01)。本试验中强筋小麦品种中麦5051在氮肥追施处理中可以兼顾籽粒产量、蛋白质含量和蛋白质产量。     

Genomic footprints of wheat evolution in China reflected by a Wheat660K SNP array

Common wheat(Triticum aestiuum L.)is one of the most important crops because it provides about 20%of the total calories for humans.T.aestiuum is an excellent modern species for studying concerted evolution of sub-genomes in polyploid species,because of its large chromosome size and three well-known genome donors.Establishment of common wheat genome reference sequence and development of high-density SNP chips provide an excellent foundation to answer questions of wheat evolution and breeding at the genomic level.By genotyping more than 600 accessions of common wheat and their diploid and tetraploid ancestors using a Wheat660 K SNP array,we found dramatic genome changes due to tetraploidization and hexaploidization,in contrast to weaker influences of domestication and breeding on them.Further,since common wheat was introduced in China in 1500 BCE,Chinese landraces formed two subgroups(T.aestiuum-L1 and T.aestiuum-L2)with considerably diverse geographic distributions and agronomic traits.T.aestiuum-L2,mainly distributed in central and east China is found to have more but smaller oval grains with early maturity characteristics.We found that variation and selection in intergenic regions of the A and B sub-genomes dominated this differentiation,in which chromosomes 7 A and 3 B took the leading roles due to the existence of putative genes related to defense responses and environmental adaption in the highly differentiated regions.Large haplotype blocks were detected on 3 B(232.6-398.3 Mb)and 7 A(211.7-272.9 Mb)in the landraces,forming two distinct haplotypes,respectively.We discovered that artificial crosses in breeding promoted recombination in the whole genome,however,this recombination and differentiation was highly asymmetric among the three sub-genomes in homoeologous regions.In addition,we found that the wide use of European and northern American cultivars in breeding at early era,led dramatic changes in Chinese wheat genome,whereas,the recent breeding functioned to optimize it.This study will provide the insight for reconsideration of wheat evolution and breeding,and a new strategy for parent selection in breeding.     

Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.