初始含水率对斥水黏壤土入渗特性的影响

为了探究初始含水率对斥水土壤入渗过程的影响规律,通过室内二维土箱的滴灌模拟试验,设置6个初始含水率水平(4.78%,7.28%,9.97%,13.64%,16.07%,19.02%),研究了初始含水率对湿润锋运移距离、宽深比、累积入渗量、入渗速率和土壤水分分布的影响,并评价了不同入渗模型的适用性.结果表明:随着初始含水率的提高,湿润锋运移相同深度所需时间呈逐渐减小趋势,两者较好满足幂函数关系,湿润锋宽深比逐渐减小;累积入渗量变化趋势为先减小后增大;入渗速率整体趋势为逐渐减小,其中斥水程度峰值含水率附近的处理出现入渗速率短暂提高的现象;Kostiakov模型能够较好反映斥水黏壤土的入渗规律,且斥水程度越大,模型拟合精度越低;随着斥水程度增加,土壤水分逐渐向湿润体垂向中间区域集中,并出现过度饱和现象.该研究可为斥水土壤的入渗理论奠定一定的基础.     

NaCl溶液处理亚热带土壤水分特征曲线差异与模型优选

携带大量盐分的低质水长期灌溉导致土壤存在极大的物理化学特性退化风险,为了探究盐分对土壤水分特征曲线影响的差异性,采用压力膜法对亚热带地区粘性潮土、沙性潮土、红壤、紫色土、水稻土等5种土壤进行室内测定,对比分析了各土壤在0、5、10、15 g/L等4个钠盐浓度水平下土壤水分特征曲线的差异,并利用RETC软件结合数理统计方法确定了各土壤不同钠盐浓度水平下相应的最优拟合模型。结果表明:钠盐处理均可提高各土壤的持水能力,且粘粒含量较高的土壤影响显著;钠盐处理减少了粘性潮土、沙性潮土和红壤的有效含水率,分别最大减少了40.8%、30.5%、31.5%,却提高了紫色土、水稻土有效含水率,分别最大提高了45.7%、28.9%。粘粒含量少或低浓度盐溶液处理的土壤水分特征曲线以BC模型拟合最优,而粘粒含量多且高浓度盐溶液处理的以DP-M模型拟合最优。     

一品红不同品种叶片叶绿素荧光特性比较

 利用叶绿素荧光技术测定了一品红4个品种‘金多利’、‘圣诞天使’、‘索诺拉飞雪’、‘旗帜’叶片光强依赖的叶绿素荧光特性。结果表明: ‘金多利’初始荧光( F_o ) 、最大荧光( F_m ) 和可变荧光( F_v ) 值极显著高于其它3个品种。在各个光强( PAR, 0～2 171μmol·m^{- 2} ·s^{- 1} ) 下, 表观电子传递速率( rETR) 、实际光化学效率(Yield) 和光化学淬灭( q_P ) 的变化趋势一致, 均为‘金多利’ > ‘圣诞天使’ > ‘索诺拉飞雪’ > ‘旗帜’, 在高PAR下这种差异更明显。拟合参数最大潜在相对电子传递速率( rETR_max ) 、半饱和光强( Ik) 和光抑制参数(β) 也同rETR等具有一致变化。说明‘金多利’有较高的PSⅡ活性, 耐强光; 而‘旗帜’则光化学效率较弱, 高光强下易受光抑制。     

柴油机功率强化前后气缸盖的温度场模拟与试

对功率强化前后的柴油机分别进行了性能仿真计算,进而确定了缸盖温度场计算的边界条件;利用有限元软件对气缸盖进行三维稳态热分析,并考虑了冷却水沸腾换热的影响;在该柴油机的单缸试验机上进行了稳态温度场测量试验,分析了试验和计算结果;得到了火力面边界条件的设置规律、稳态温度场随工况的变化关系和单缸机与多缸机的稳态温度场差别.     

四川盆地丘陵区土壤水分垂直变异特征研究

根据四川盆地丘陵区重庆市璧山县青杠墒情站点土壤水分每天的动态监测资料,用小波分析的谱相分析方法研究了土壤水分的垂直异质性。结果表明:土壤水分变化是个随机平稳的波动序列;就时间变异来看,其变异有一定的隐周期,大致可分为3~5月的次波动期、7~9月的剧烈波动期、10~2月的相对稳定期。就土壤的垂向变化来看,站点的0~10 cm的变异高于10~20、20~40 cm层变异;各层变异之间存在一定的因果关系,可用互谱密度系数表示,但受降水、蒸发、作物利用等多种因素的制约,并未表现出完全的同步响应关系。     

Interleukin-1β inhibits AKT to down-regulate eNOS Ser1177 phosphorylation in human umbilical vein endothelial cells

AIM To investigate whether interleukin-1β （IL-1β） regulates endothelial nitric oxide synthase （eNOS） phosphorylation at Ser1177 site in human umbilical vein endothelial cells （HUVECs）, and to explore its possible mechanism. METHODS The HUVECs were randomly divided into normal control group, tumor necrosis factor-α （TNF-α） group, IL-1β group, IL-6 group, SC79 ［protein kinase B （PKB/AKT） specific agonist］ group and SC79+IL-1β group. Western blot was used to determine the protein levels of eNOS, p-eNOS-Ser1177, AKT and p-AKT-Ser473 in the HUVECs. Chemical colorimetry was used to detect the nitric oxide （NO） content in the culture medium of HUVECs. RESULTS No statistically significant difference of p-eNOS-Ser1177 level in HUVECs treated with TNF-α and IL-6 was observed as compared with normal control group （P>0.05）, while the protein level of p-eNOS-Ser1177 in the HUVECs and the content of NO in the culture medium of HUVECs decreased significantly in IL-1β group （P<0.05）, and the protein level of p-AKT-Ser473 in the HUVECs was decreased as compared with normal control group （P<0.05）. The AKT agonist SC79 blocked the down-regulation effect of IL-1β on p-eNOS-Ser1177 level in the HUVECs and NO content in the culture medium of HUVECs （P<0.05）. CONCLUSION IL-1β down-regulates the protein level of p-eNOS-Ser1177 in HUVECs and affects the activity of eNOS, which may be involved in AKT/eNOS signaling pathway.     

甘蔗叶不同还田方式对土壤养分的影响

为减轻秸秆焚烧所带来的环境问题和充分利用甘蔗叶资源,在大田环境下动态监测甘蔗叶粉碎、焚烧和深埋3种还田方式对土壤养分的影响。结果表明:甘蔗叶粉碎还田和深埋还田有利于甘蔗叶中有机碳及氮、磷、钾养分的缓慢释放;甘蔗叶焚烧还田提高了土壤碱解氮含量5.6~20.9 mg/kg和速效磷含量0.8~4.0 mg/kg,促进土壤中氮磷向有效态转化,焚烧还田初期显著增加了土壤速效钾的含量12.3~18.3 mg/kg,但焚烧会损失部分甘蔗叶中碳氮元素,也造成环境污染。综合分析认为甘蔗叶粉碎还田是比较省工、方便的处理方式。     

Optimization of curing process for polymer-matrix composites based on orthogonal experimental method

In this paper, the orthogonal experimental method was carried out to optimize the curing process of aeronautical composite X850/T800 in autoclave process. Four important curing parameters including curing pressure, heating rate, curing temperature and heat preservation time were taken into account, and sixteen samples were fabricated to study the effects of the four parameters mentioned above on the curing quality by interlaminar properties test and microstructure analysis. The interlaminar properties and the interfacial bonding quality of these samples were studied by the short-beam three points bending test and scanning electron microscopy, respectively. Results revealed that the optimal curing process of X850/T800 composite laminate should be as follows: curing pressure of 0.6 MPa, heating rate of 1.5 °C/min, curing temperature of 160 °C, and heat preservation time of 120 min.     

Development and application of a molecular marker for TMV resistance based on <Emphasis Type="Italic">N</Emphasis> gene in tobacco (<Emphasis Type="Italic">Nicotiana tabacum</Emphasis>)

Tobacco mosaic virus (TMV) caused serious loss in yield and quality of tobacco every year. It is a long-term goal to improve the tobacco resistance against TMV by tobacco breeding. N gene was the firstly reported TMV-resistant gene, which showed resistance against all Tobamoviruses except the Ob stain and belonged to the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant resistance (R) genes. At present, N gene had already been widely used in tobacco conventional breeding, but there is rare available molecular maker used in marker-assisted selection of TMV resistance. In this study, we designed a pair of primers that specific amplify N gene fragment based on the sequence of N gene intron III, named N-marker. Then, we identified TMV resistance by two selecting methods, PCR with N-marker and inoculated with the TMV-C strain. Results from the two method showed that (1) 13 varieties among 67 tobacco varieties displayed hypersensitive reaction when inoculated with the TMV-C strain, also contained N gene fragments screened by PCR with N-marker; (2) 105 strains of 200 BC1 strains showed resistance against TMV when inoculated with TMV-C strain, meanwhile, 103 of the 105 strains contained N gene fragment verified by PCR with N-marker. Therefore, the N-marker is reliable for high throughput screening of germplasm resources and tobacco breeding materials in selection of N-mediated TMV resistance. Our study not only developed a molecular marker for tobacco breeding, but also identified new germplasm resources that are resistant to TMV.     

Two reported cytotypes of the emergent orchid model species <Emphasis Type="Italic">Erycina pusilla</Emphasis> are two different species

Each species is characterized by a specific set of chromosomes, which is described as the chromosome portrait or karyotype. In general, such a karyotype is the same for all individuals in the population. An exception to that rule has recently been found in the orchid Erycina pusilla, which has been reported to have two cytotypes with chromosome numbers of 2n = 10 and 2n = 12. Here, we examined the karyotypes of the two cytotypes and found differences in arm ratios and heterochromatin patterns as well as in the presence of satellite chromosomes and in the number and location of rDNA and telomeric repeat sites. These differences are extensive and would have required multiple chromosome rearrangements to generate the differences between the two karyotypes. We also found that F1 hybrids between the parents with the two different chromosome numbers resulted in sterile offspring, in accordance with our previous findings. The combination of hybrid sterility and extensively rearranged chromosomes supports the hypothesis that these two reported cytotypes are, in fact, two different species.