Forest responses to climate change in the northwestern United States: Ecophysiological foundations for adaptive management

Climate change resulting from increased concentrations of atmospheric carbon dioxide ([CO₂]) is expected to result in warmer temperatures and changed precipitation regimes during this century. In the northwestern U.S., these changes will likely decrease snowpack, cause earlier snowmelt, increase summer evapotranspiration, and increase the frequency and severity of droughts. Elevated [CO₂] and warmer temperatures may have positive effects on growth and productivity where there is adequate moisture or growth is currently limited by cold. However, the effects of climate change are generally expected to reduce growth and survival, predispose forests to disturbance by wildfire, insects, and disease; and ultimately change forest structure and composition at the landscape scale. Substantial warming will likely decrease winter chilling resulting in delayed bud burst, and adversely affect flowering and seed germination for some species. The extent of these effects will depend on the magnitude of climate change, the abilities of individual trees to acclimate, and for tree populations to adapt in situ, or to migrate to suitable habitats. These coping mechanisms may be insufficient to maintain optimal fitness of tree populations to rapidly changing climate. Physiological responses to climatic stresses are relatively well-understood at the organ or whole-plant scale but not at the stand or landscape scale. In particular, the interactive effects of multiple stressors is not well known. Genetic and silvicultural approaches to increase adaptive capacities and to decrease climate-related vulnerabilities of forests can be based on ecophysiological knowledge. Effective approaches to climate adaptation will likely include assisted migration of species and populations, and density management. Use of these approaches to increase forest resistance and resilience at the landscape scale requires a better understanding of species adaptations, within-species genetic variation, and the mitigating effects of silvicultural treatments.     

Clinical signs associated with brain tumors in dogs: 97 cases (1992-1997).

OBJECTIVE: To determine the prevalence of various clinical signs in dogs with brain tumors. DESIGN: Retrospective study. ANIMALS: 97 dogs with brain tumors. PROCEDURE: Medical records were reviewed for signalment, tumor type and location, and clinical signs. RESULTS: 33 breeds were represented; Golden Retrievers were most commonly affected. Most dogs were older (median age, 9 years); 95% of dogs were > or = 5 years old. Seventy-six percent of dogs had tumors in the supratentorial region. Seizures were the most common clinical sign at initial examination, with lower prevalence for circling, ataxia, and head tilt. Meningioma was the most common tumor. CONCLUSIONS AND CLINICAL RELEVANCE: Brain tumors develop most often in dogs > or = 5 years old and are uncommon in dogs < 5 years old. Seizures are a common clinical sign, and a brain tumor should be considered in dogs that have their first seizure after they are 4 years old.     

Characterization of the immune response of domestic fowl following immunization with proteins extracted from Dermanyssus gallinae

Dermanyssus gallinae is the most significant ectoparasite of European poultry egg laying production systems due to high costs of control and associated production losses as well as adverse effects on bird welfare. In this study, soluble proteins were extracted from unfed D. gallinae (DGE) using a urea-based detergent and ultra-filtration, passed through a 0.22 μm filter and blended aseptically with adjuvant. One group of laying hens was immunized with DGE and adjuvant (Montanide ISA 50 V) whilst another group (Control) received physiological saline and adjuvant. All birds were immunized on two occasions, 21 days apart. Antibody response to immunization was determined by ELISA and western blotting using immunoglobulins (Igs) extracted from egg yolk. DGE immunization of hens resulted in a significant (P < 0.05) IgY response compared to controls, although there was no significant difference in IgM response between treatments. A number of proteins were identified by western blotting using IgY antibodies from DGE immunized birds, most prominently at 40 and 230 kDa. Analysis of proteins from approximately corresponding bands on SDS-PAGE confirmed the identity of tropomyosin, whilst other proteins showed high sequence homology with myosin and actin from other arachnid and insect species. Immunization of hens with DGE resulted in a 50.6% increase in mite mortality (P < 0.001) 17 h after feeding when tested by an in vitro mite feeding model. Data in this study demonstrate that somatic antigens from D. gallinae can be used to stimulate a protective immune response in laying hens. Further work is needed to identify other proteins of interest that could confer higher protection against D. gallinae, as well as optimization of the vaccination and in vitro testing protocol.     

Forests planted for ecosystem restoration or conservation

Although the phrase, planting for ecosystem restoration, is of recent origin, many of the earliest large-scale tree plantings were made for what we now refer to as restoration or conservation goals. Forest restoration activities may be needed when ecosystems are disturbed by either natural or anthropogenic forces. Disturbances can impact (1) basic components of the system (e.g., plant and animal composition, soil pools, and atmospheric pools), (2) ecosystem processes, i.e., interactions among basic components, or (3) both components and processes. Early efforts at restoration or site rehabilitation focused primarily on reducing off-site impacts, such as sediment introduced into streams from ecosystems that had been severely disturbed. More recent restoration programs include ecosystems in which only some of the components are missing or some of the processes have been impacted. Restoration activities can begin immediately after the disturbance has ended. Although forest restoration projects can include many activities, planting is almost always a key component.When planning an ecosystem restoration project, land managers need to be aware that commonly used plant establishment and management procedures may need to be altered to meet project objectives. Some systems may have been so severely impacted that ameliorative activities, e.g., fertilization, liming, land contouring, and microsite preparation, will be necessary prior to planting. Managers may also need to take special measures to reduce herbivory, control competing vegetation, or reduce physical damage from wind or sun. Choice of species needs careful consideration. Desired species may not grow well on degraded sites, may need a nurse species to become established, or may not provide an opportunity to harvest a short-term crop to reduce restoration costs. New methods may need to be developed for projects that require underplanting or interplanting. The end result of restoration should be an ecosystem with the same level of heterogeneity inherent in an undisturbed system; thus, managers should consider how pre- and postplanting activities will affect system variability.As our understanding of ecosystems has increased, so has our expectation that restored ecosystems have the same components and function in the same manner as do undisturbed systems. These expectations require that land managers have more sophisticated information than was considered necessary previously. In the absence of more pertinent information, we can prescribe restoration activities based on results from related ecosystems or on theoretical considerations. Additional research, careful monitoring, and adaptive management are critical to our long-term success.     

Evaluation of a real-time quantitative polymerase chain reaction assay for detection and quantitation of virulent Rhodococcus equi

OBJECTIVE: To evaluate a real-time quantitative polymerase chain reaction (QPCR) assay in the detection and quantitation of virulent Rhodococcus equi. SAMPLE POPULATION: 1 virulent, 2 intermediately virulent, and 2 avirulent strains of R. equi and 16 isolates of bacteria genetically related to R. equi. PROCEDURE: The QPCR assay was evaluated for detection and quantitation of the virulence-associated gene (vapA) of R. equi in pure culture and in samples of tracheobronchial fluid, which were inoculated with known numbers of virulent R. equi. Results were compared with those derived via quantitative microbial culture and standard polymerase chain reaction methods. RESULTS: The QPCR assay detected the vapA gene in pure culture of R. equi and in tracheobronchial fluid samples that contained as few as 20 CFUs of virulent R. equi/mL and accurately quantitated virulent R. equi to 10(3) CFUs/mL of fluid. The assay was highly specific for detection of the vapA gene of virulent R. equi and was more sensitive than standard polymerase chain reaction for detection of R. equi in tracheobronchial fluid. CONCLUSIONS AND CLINICAL RELEVANCE: The QPCR assay appears to be a rapid and reliable method for detecting and quantitating virulent R. equi. The accuracy of the QPCR assay is comparable to that of quantitative microbial culture. The increased sensitivity of the QPCR method in detection of virulent R. equi should facilitate rapid and accurate diagnosis of R. equi pneumonia in foals.