Decreased biological nitrogen fixation in glyphosate-resistant (GR) soybeans has been attributed directly to toxicity of glyphosate or its metabolites, to N2-fixing microorganisms. As a strong metal chelator, glyphosate could influence symbiotic N2 fixation by lowering the concentration of nickel (Ni) that is essential for the symbiotic microorganisms. Evaluation of different cultivars grown on different soil types at the State University of Maringá, PR, Brazil during the summer 2008 revealed, significant decreases in photosynthetic parameters (chlorophyll, photosynthetic rate, transpiration and stomatal conductance) and nickel content with glyphosate use (single or sequential application). This work demonstrated that glyphosate can influence the symbiotic N2 fixation by lowering nickel content available to the symbiotic microorganisms. 相似文献
The epidemiology of the Neotropical rust caused by Puccinia psidii in the Indian Myrtaceae Syzygium jambos (rose apple) was investigated in central Brazil. Disease severities recorded during a 12-month period were fitted by a Fourier curve with three cyclic components or harmonics. The first, second and third harmonics accounted for 49·6, 25·9, and 1·5% of total disease measured, respectively. A highly significant linear correlation was observed between the first harmonic and the accumulated number of days having at least 6 h wetness, or a minimum of 90% relative humidity, combined with temperatures that ranged from 18 to 20°C. No significant correlation was observed between the second and third harmonics and the weather variables evaluated. Rose apple trees showed a flush of growth with new susceptible host tissue during the evaluation period, with two major outbreaks of rust of variable intensity. Host growth was fitted by a Fourier curve with two significant harmonics. The first and second harmonics accounted for 37·5 and 22·1% of total host growth measured, respectively. A highly significant negative linear correlation was observed between the first harmonic and daily maximum and minimum temperatures, rainfall, and duration of the light period. Inoculum availability was not a limiting factor for disease progress since urediniospores were present during most of the period studied. A major peak in numbers of rust spores followed the main peak of disease severity. Thus, rust epidemics on rose apple in central Brazil were shown to depend on the duration of leaf wetness in the dark, and also on night-time temperatures during that same wetness period. This study is the first example of a periodical analysis of an epidemic in a perennial crop. 相似文献
Canine hepatozoonosis is a disease caused by the tick-borne protozoan Hepatozoon spp. It has been reported in the United States, southern Europe, the Middle East, Africa and the Far East. In Brazil, canine hepatozoonosis is an emerging protozoal tick-borne disease, and is characterized by distinct clinical signs. The objective of this study was to analyze the laboratory findings of some hepatozoonosis cases in dogs in Brasília, Brazil, and their clinical signs. The animals of this experiment showed low parasitemia, similar to H. americanum, but the clinical signs presented were similar to H. canis. According to our observations and in agreement with O'Dwyer et al. [Vet. Parasitol. 94 (2001) 143], the Brazilian Hepatozoon appears more to resemble the species found in the eastern Hemisphere than with H. americanum of North America, or could be caused by a new species. Our data revealed that hepatozoonosis could be considered endemic in Brasília. 相似文献
The chiral inversion process is a characteristic metabolic pathway for different aryl-2-propionic acids or profens. Important variations have been observed between these individual compounds as well as between animal species. In this study, R(-) fenoprofen [R(-)FPF] and R(-) ketoprofen [R(-) KTF] were used to investigate their comparative stereoconversion in cats. After intravenous (i.v.) administration of R(-) FPF, the percentage of chiral inversion was 93.20+/-13.70%. A highly significant correlation (r: 0.978) was observed between the clearance of R(-) FPF and the chiral inversion process. After i.v. administration of R(-) KTF, the percentage of inversion was only 36.73+/-2.8%. No correlation between the clearance of R(-) KTF and this process was observed. R(-) FPF was metabolized by the pathways of thioesterification - chiral inversion processes. For R(-) KTF, the competitive metabolic pathways, glucuronidation and hydroxylation may be involved. However, these metabolic steps are saturable or less functional in cats. Moreover, the thioesterification of R(-) KTF in in vitro studies has been shown to be important in carnivores. The lack of correlation between clearance and chiral inversion process of R(-) KTF may be finally explained by deviation of thioesterification to other metabolic pathways of lipids and/or aminoacid conjugation, particulary glicine derivatives. 相似文献
In vitro viability of purified Cryptosporidium parvum oocysts, exposed for 30, 60, 90 and 120 min to 0.27 mg/ml lasalocid suspension was evaluated by inclusion or exclusion of two fluorogenic vital dyes and an excystation technique. Continuously, preventive and curative efficacies at different doses (9, 6.75, 5.625 and 4.5 mg/kg body weight) and regimens of lasalocid against cryptosporidial infection were evaluated on an experimental neonatal mice model. In vitro assays demonstrated a decrease in the oocyst viability related to an increase in exposure time for exposure to the lasalocid suspension. The infection was eradicated when the suspension was administered with a dose of ≥6.75 mg/kg body weight. No apparent toxic effects were observed. 相似文献
An 8-year-old, entire male crossbreed dog had a 2-day history of abdominal pain and straining to urinate and defecate. A diagnosis of urolithiasis with urinary retention, and probable prostatitis was made. The dog was treated with fluids and enrofloxacin. Five days later, ultrasonography of the prostate showed two hypoechoic areas compatible with abscesses. Twelve hours later, the patient started to urinate from the rectum during micturition and urethrorectal fistula was diagnosed. The omentalization of the prostatic abscesses and castration were performed and calculi were removed. The urethrorectal fistula was treated conservatively with an indwelling urinary catheter, a low residue diet and antibiotics. The dog recovered uneventfully without recurrence of the fistula. 相似文献
The combined effect of drought and light on different physiological and biochemical traits was assessed in cork oak (Quercus suber L.) seedlings grown under two levels of light availability and submitted to a long-standing drought. Watering was withdrawn after germination and seedlings were allowed to dry to a water content of ca. 50% of field capacity. At this point, water-stressed seedlings were grown under moderate drought and two light regimes: high light (HL—50%) and low light (LL—2%). Soil water in control plants was kept close to field capacity (90–100%) for both light environments. Water-relations parameters derived from P–V curves, gas exchange and water status at predawn (Ψpd) were evaluated at twice during the experiment. Nitrogen and chlorophyll contents were determined in the same leaves used for the gas exchange measurements. In addition, maximum rate of carboxylation (Vcmax) and electronic transport (Jmax) were derived from A–Ci curves in well-watered seedlings.
The variation on moisture availability during the experiment was the same under both light environments. In control plants, Ψpd was over −0.3 MPa at the two harvests, while stressed seedlings decreased to −0.9 MPa, with no differences between light treatments. Water stress decreased osmotic potentials at full (Ψπ100) and zero turgor (Ψπ0). The regressions between both potentials and Ψpd showed a higher intercept in shade grown seedlings. This fact will point out the higher osmoregulation capacity in sun seedlings whatever water availability.
Nitrogen investment on a per leaf mass (Nmass), chlorophyll content (Chlmass) and SLA tended to show a typical pattern of sun-shade acclimation. Thus, the three parameters increased with shade. Only for Nmass there was a significant effect of watering, since water stress increased Nmass.
LL plants showed a lower photosynthetic capacity in terms of maximum net photosynthesis at saturating light (Amax), which was related to a decrease in Vcmax and Jmax. Both parameters varied with specific leaf area (SLA) in a similar way. The low-light environment brought about a higher nitrogen investment in chlorophyll, while under high-light environment the investment was higher in carboxylation (Vcmax) and electronic transport (Fmax).
Stomatal conductance to water vapour (gwv) and Amax were lower in low-light seedlings independently of watering. In addition, there was a trend to keep higher intrinsic water use efficiency (IWUE) under high light environment. The increase of IWUE under water stress was higher in HL seedlings. This was as consequence of the steeper decline in gwv as Ψpd decreased. The decrease of Amax with Ψpd occurred in a similar way in LL and HL seedlings. Thus, the HL seedlings tended to sustain a higher ability to increase IWUE than LL seedlings when they were submitted to the same water stress. 相似文献