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771.
772.
Pathogenicity, vegetative compatibility and RAPD analysis of Fusarium oxysporum isolates from tobacco fields in Extremadura 总被引:1,自引:0,他引:1
Mª Carmen Rodríguez-Molina Mª Carmen Morales-Rodríguez Carolina Palo Mª Dolores Osuna Mª José Iglesias José Antonio García 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(3):639-650
Fusarium wilt of tobacco could be caused by Fusarium oxysporum f. sp. batatas or f. sp. vasinfectum since f. sp. nicotianae was rejected because there was no evidence of isolates specific to tobacco. Forty isolates of F. oxysporum from soil and plants from tobacco fields in Extremadura (south-western Spain) were characterized by pathogenicity on burley and flue-cured tobacco, for vegetative compatibility group (VCG), and by random amplified polymorphic DNA (RAPD). Isolates from burley were identified as race 1 of F. oxysporum f. sp. batatas based on pathogenicity on tobacco, sweet potato and cotton, and those from flue-cured as race 2. Most isolates from soil were heterokaryon self-incompatible (HSI) and the remaining isolates from soil and tobacco were grouped into four VCGs: VCG 1 (5 isolates from burley), VCG 2 (17 isolates from flue-cured and 4 from soil), VCG 3 (2 isolates from flue-cured) and VCG 4 (2 isolates from soil). This is the first report of the two races and VCGs of F. oxysporum f. sp. batatas in Spain. Analysis of RAPD revealed two clusters (C-I and C-II) related to race and VCGs. C-I included race 1 (VCG 1) isolates from burley and nonpathogenic (VCG 4 or HSI) isolates from soils. C-II included nonpathogenic (VCG 2) and race 2 (VCG 2 or VCG 3) isolates from flue-cured. VCG and RAPD markers were effective in distinguishing race 2 from race 1, suggesting that there are two genetically differentiated groups of F. oxysporum f. sp. batatas on tobacco in Extremadura. 相似文献
773.
Carmen Martín Angelika Senula Iván González Andrés Acosta E. R. Joachim Keller M. Elena González-Benito 《Genetic Resources and Crop Evolution》2013,60(1):243-249
At the genebank of IPK (Leibniz Institute of Plant Genetics and Crop Plant Research) in Gatersleben, Germany, three long-term conservation methods (field genebank, in vitro slow-growth and cryopreservation) are used for mint germplasm. The plant material of the field genebank was the source to establish the slow-growth in vitro culture collection, from which the cryopreserved collection was set up, using a droplet-vitrification protocol. The genetic identity of 161 samples of three mint accessions (MEN 198, MEN 166 and MEN 186), stored for several years using those three methods, was studied using RAPD markers. Accession ‘MEN 198’ was the only one with a unique RAPD fragment pattern for all its samples, independently of the conservation procedure employed. The field collections of accessions ‘MEN 166’ and ‘MEN 186’ were made up of different genotypes. None of the genotypes detected in the plots of these accessions was represented in the in vitro and cryopreserved samples analyzed, which showed a unique genotype for each accession. From this work we conclude that, for an appropriate management of a germplasm collection of vegetatively propagated species, the determination of the genetic composition of the donor material and a periodical assessment of the preserved material should be carried out. 相似文献
774.
沙棘果、种子和叶片中的大量营养和药用价值已经被长篇累牍地报道。众所周知的源自沙棘果实的沙棘油,目前被利用作为护肤品中的一种抗氧化剂和防紫外线成分或食品增补剂。 相似文献