马铃薯原生质体培养体系改良

试验以马铃薯试管苗叶片为原生质体来源，通过研究材料预处理方法对原生质体产量和质量的影响，培养方式及培养基中糖醇与细胞分裂的愈伤组织形成的关系，优化并建立了高效稳定的原生质体培养体系，获得了2个双单倍体系的叶肉原生质体再生植株。     

磷素水平对不同大豆品种钾素吸收效率的影响

通过原子吸收分光光度计法研究了施磷量对不同大豆品种各器官及全株吸收钾素营养的影响,结果表明:施磷量对不同大豆品种植株及各器官钾素含量有较大影响.不同品种不同处理全株及各器官钾素含量从分枝期逐渐增加,开花期达到高峰,随后下降至成熟期;同一品种不同处理间钾素含量三个品种都是P10处理全株及各器官钾素含量最高,整个生育期高磷或不施磷都会影响钾素含量,只有适宜的施磷才能促进钾素含量达到最高峰.同一处理不同品种全株及各器官钾素含量在品种间没有明显的变化规律.     

大豆铁蛋白cDNA的克隆及植物表达载体的构建

以大豆为材料,利用RT-PCR法扩增得到大小约750bp的片段,将这个片段连接到克隆载体pBlueskript SK＋上进行测序,结果证明,此片段就是大豆的铁蛋白基因。将此片段再正向插入到植物表达载体pBI121的CaMV35s启动子和NOS终止子之间,构建了植物表达载体pBIFe,并成功地将该载体导入根癌农杆菌EHA105。此项工作为获得表达铁蛋白的转基因植物奠定了基础。     

桉树尺蠖幼虫不同危害时期施药防治效果比较

桉树尺蠖是闽南地区桉树林中发生最严重的食叶害虫,为控制其危害,确定最佳防治适期,通过采用林间喷洒2％阿维菌素+苏云金杆菌粉剂的方法,比较第1代幼虫危害早期(5月2日)、危害中期(5月13日)和危害后期(5月30日)分别进行防治的效果,结果表明:在第1代尺蠖幼虫危害早期,每667m2使用500 g药剂进行防治,5 d后虫...     

浅谈牛前胃疾病的中西医治疗

牛前胃疾病是兽医临床上常见的牛瘤胃、网胃、瓣胃疾病的总称,是由于饲料加工调制不当,金属异物进入网胃,导致网胃和腹膜损伤产生炎症;或饲养管理、使役不当等原因导致胃神经兴奋性降低,胃收缩无力甚至停止,食物在前胃不能正常消化和向后移动而腐败分解,产生有毒物质,引起消化机能障碍和全身机能紊乱的多发性内科疾病.     

Physiological and anatomical changes in two rapeseed (Brassica napus L.) genotypes under drought stress conditions

Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed (Brassica napus L.) varieties. The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes, P287 (drought-tolerant) and T88 (drought-sensitive) under three intensities of drought stress. All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions. At the fourth-leaf stage, the relative water content, chlorophyll content, protein content, malondialdehyde content, and the activities of peroxidase and catalase in P287 were significantly higher than those in T88, particularly under severe drought conditions. After rehydration, all physiological indexes recovered rapidly, especially in P287. In addition, under drought stress, compared with T88, P287 had thicker palisade tissue, thinner spongy tissue, higher ratio of chloroplast length to chloroplast width, higher stomatal density and stomatal closure rate. Overall, the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.     

氧化-生物双降解地膜对棉花生长影响及降解效果

研究氧化-生物双降解地膜对棉花生长的影响及降解效果,为氧化-生物双降解地膜代替普通聚乙烯地膜的可行性及推广应用提供理论参考。试验采用2种不同配方的氧化-生物双降解地膜,以普通聚乙烯地膜作为对照,分析不同降解地膜对棉花生长及产量的影响,观测增温保墒效果,明确降解性能。结果表明：2种降解地膜对棉花生育期、农艺性状、产量性状和纤维品质未产生显著影响,籽棉产量和增温保墒效果与普通地膜基本相同;诱导期为39 d的2018新疆阿拉尔棉花1号降解膜降解率达47.3 %,显著高于其他处理,具有较高的应用价值。     

Effects of calpain-2 and autophagy-related protein 5 on hepatocyte apoptosis induced by endoplasmic reticulum stress

AIMTo investigate the effects of calpain-2 and autophagy-related protein 5 (Atg5) on apoptosis of BRL-3A rat normal liver cells during endoplasmic reticulum stress (ERS) induced by dithiothreitol (DTT). METH?ODS: BRL-3A cells were treated with DTT at 2.0 mmol/L for 0, 6, 12 and 24 h to induce ERS. Real-time cell analysis (RTCA) was used to measure the effect of DTT on BRL-3A cell proliferation. Apoptosis and cell cycle distribution were analyzed by flow cytometry. The mRNA expression of calpain-2 and Atg5 was detected by real-time PCR. The protein levels of calpain-2, Atg5, Atg7, Atg12 and microtubule-associated protein 1 light chain 3 (LC3) were determined by Western blot. The interaction between calpain-2 and Atg5 was investigated by co-immunoprecipitation (Co-IP). RESULTSThe proliferation of BRL-3A cells treated with DTT was significantly inhibited. The apoptosis of BRL-3A cells was significantly increased after DTT treatment for 6, 12 and 24 h as compared with 0 h group (P<0.05). The cell cycle was arrested in G₁ phase after DTT treatment (P<0.05). After DTT treatment for 6, 12 and 24 h, the mRNA expression of calpain-2 and Atg5 in the BRL-3A cells was significantly increased as compared with 0 h group (P<0.05). The protein levels of calpain-2, Atg12 and Atg7 in the cells treated with DTT for 6, 12 and 24 h were significantly higher than those in 0 h group, and the ratio of LC3-II/LC3-I was also significantly higher than that in 0 h group, while Atg5 expression was significantly lower than that in 0 h group (P<0.05). The results of Co-IP found that the anti-calpain-2 antibody precipitated Atg5 protein from the cell lysates, and the anti-Atg5 antibody also precipitated calpain-2 from the cell lysates, which confirmed the interaction between calpain-2 and Atg5. CONCLUSION Calpain-2 may participate in ERS-induced hepatocyte apoptosis by interacting with Atg5.