商品肉鸡非典型新城疫的防制

近年来,随着我国农村肉鸡养殖业的较快发展,养殖户对新城疫（ND）的免疫预防都非常重视,普遍进行了ND的免疫接种.因此,典型的ND已很难再见到,剖检时也很少再见到典型ND的特征性病变,给ND的诊断和防制带来了一定的难度,同时给农村养鸡户带来了很大的经济损失.     

猪繁殖和呼吸综合征病毒分子生物学研究进展

猪繁殖和呼吸综合征病毒（ＰＲＲＳＶ）是引起母猪流产和仔猪呼吸道症状及死亡的一种新的重要病原。该病毒于１９９１年７月首先由荷兰分离获得（当时,称之为Ｌｅｌｙｓｔａｄ病毒,ＬＶ）,接着,德国、美国、比利时、英国、苏格兰、法国、西班牙、日本、韩国及台湾等很...     

海南50个甘蔗品种黄叶病毒的分子鉴定

为明确国家甘蔗体系集成示范及区试甘蔗品种在海南省被甘蔗黄叶病毒(SCYLV)侵染情况及病毒基因型类型,从集成示范及区试的50个甘蔗品种上采集带有显著病症或不显病症样品50份,采用特异引物通过RT-PCR方法进行甘蔗黄叶病毒检测和病毒基因型鉴定。结果表明:50个甘蔗品种中有31个被检测到SCYLV,检出率为62%;病毒基因型有古巴(CUP)基因型、巴西-秘鲁(BAR-PER)基因型和留尼汪岛(REU)基因型3种,主要以CUP基因型为主,占58.06%,其次是BAR-PER基因型,占51.61%,REU基因型最少,占29.03%;未发现CHN1、CHN2、CHN3基因型。另外,不同基因型混合侵染现象普遍存在,总混合侵染率达32.26%。可见,海南国家甘蔗体系集成示范及区试甘蔗品种严重感染甘蔗黄叶病毒(SCYLV),且存在不同基因型混合侵染,建议推广种植脱毒种苗来控制甘蔗黄叶病的发生。本研究为在海南蔗区推广和种植甘蔗脱毒种苗及抗病育种提供了理论支持。      

Induction of interspecific allo-tetraploids of Megalobrama amblycephala♀×Megalobrama terminalis♂ by heat shock

Interspecific allo-tetraploidy was induced by a combination of hybridization of Megalobrama amblycephala ♀× Megalobrama terminalis ♂ and thermal shock. A thermal shock at 40 °C for 2 min applied to eggs 33 or 26 min (τ₀: 1.40) after fertilization resulted in a significantly ( P <0.01) higher yield of allo-tetraploids than hybrid crosses without heat shock. The yield of allo-tetraploids in embryos from mid-gastrula to hatching stages was 15.7–24.4% in different thermal shock treatment groups. The allo-tetraploidy rate in 330-day-old juveniles was 9.3% and 6.7% in different year treatment groups. After 2 years of rearing, allo-tetraploidy males attained sexual maturity with milk-like milt at the age of 2+, while allo-tetraploidy females at the age of 2+, and even at 3+, did not show any signs of sexual maturation exhibiting obviously delayed maturity. Only a few allo-tetraploidy females (three individuals) showed maturation characters at the age of 4+ and these females were induced to spawn.     

库鄯线减压站清管作业防堵方案

介绍了库鄯线觉罗塔格减压站的主要功能和库鄯线的特点，分析了在历次清管作业中觉 罗格格减压站多次发生堵塞的原因，依据相关技术研究成果及历次清管作业经验，研究并探讨了合理的清管作业方案及防止减压站蜡油阻塞的技术和措施。     

Feeding selectivity of the marine ascidian Styela clava

The sessile suspension feeding ascidian, Styela clava , plays an important role in the functioning of estuarine systems. The feeding selectivity of S. clava was examined using standard physiological techniques in terms of particle sizes and diet concentrations. Styela clava increased its retention efficiency (the proportion of available particles retained in ascidian) with increasing particle size at a lower diet concentration [expressed as particulate organic matter (POM)] from 2.11 to 10.06 mg POM L⁻¹, and the highest retention was reached for 5–20 μm particles. With increasing diet concentration to 20.12 mg POM L⁻¹, S. clava retained small particles (<3.5 μm) more efficiently and large ones (>15 μm) less efficiently. The selectivity of ascidian with different sizes was similar at the same concentration. At every diet concentration, larger ascidians retained more 5–20 μm cells than the smaller ones. All these results indicate that S. clava possesses a particle selection mechanism that may affect the zooplankton communities to a certain degree.     

创设会计实践教学情境的应用

会计实践教学情境是对会计核算的仿真模拟具有重要意义。会计实践教学情境要考虑会计核算的内、外部环境，在此基础上，确定会计岗位的分工和职责。按照会计业务的合理流程，设置出纳、会计等适当的岗位，并根据每个岗位的特点设计与之相适应的会计业务，培养适应社会经济发展需求的会计人才。     

野外昆虫与蚕种生产中的微粒子病防治

家蚕体内分离发现的 5种微粒子虫中 ,除家蚕微粒子虫 ( N osema bombycis)外未发现对家蚕具有较强致病性和胚传性的微粒子虫。野外昆虫中已有大量微粒子虫被发现 ,其中有些对家蚕也有很强的致病性和胚传性 ,但其原始寄主尚有诸多不明之处。所以 ,蚕种生产中防止野外昆虫微粒子虫对生产影响的根本途径在于控制家蚕微粒子虫污染的扩散和桑园治虫     

黔苦2号苦荞新品种春播高产综合农艺措施研究

运用二次回归正交旋转组合设计,在春播条件下研究黔苦2号苦荞籽粒产量与其播种量及N、P、K肥施用量间的关系.通过试验,建立了黔苦2号籽粒产量与主要农艺措施的数学模型,通过对模型的解析和模拟寻优分析.结果表明:黔苦2号籽粒产量高于2800kg/hm2的优化栽培技术方案:播种量67.14～74.93 kg/hm2,施N量28.88～40.12 kg/hm2,施P2O5量179.11～210.89 kg/hm2,施K2O量43.94～61.06kg/hm2;试验4因子对籽粒产量影响的程度大小为播种量>施K2O量>施P2O5量>施N量.     

预处理对SFE提取月见草种子、被孢霉菌丝体油脂的影响

以月见草种子、被孢霉菌丝体为超临界流体萃取(SFE)的试验材料,研究了不同预处理条件(水分、粉碎度、粉碎方式)在不同萃取压力下对超临界流体萃取油脂得率的影响;确定粉碎度、粉碎方式、水分与油脂萃取之间的关系。