杂种优势理论研究的回顾与展望

杂种优势是生物界的普遍现象，目前，其遗传理论的发展远落后实践中所取得的成就，这就限制了杂种优势的进一步利用和预测。本文在论述杂种优势遗传理论研究现状的基础上，对今后的发展作了展望。     

高寒牧区碱茅＋杂花苜蓿混播试验初报

为选择适合在高海拔，寒冷地区推广的多年生混播牧草品种，采用近年培育出的甘农１号杂花苜蓿与碱茅，在肃南县进行了混播试验，结果表明，高寒地区碱茅与甘农１号杂花苜蓿混播有较强的抗寒性和越冬能力，碱茅与苜蓿以７：４比例混播，可获得最佳产草量和牧草品质。     

防治葡萄黑痘病的苗木消毒试验初报

 葡萄黑痘病(Elsinoe ampelina(de Bary) Shear)为我国北部葡萄的主要病害,近年在各地造成严重减产,并不断随着苗木蔓延到无病的新区,因此它是当前葡萄病害防治和检疫的主要对象。     

红砒处理麦种防治黑穗的试验

 红砒(信石、红攀或红盆)和白砒(砒霜或信精)可以说是同样的物质。     

Booroola绵羊内分泌学研究进展Ⅱ.性腺轴的结果

从性腺轴角度综述了Booroola美利奴绵羊内分泌学研究进展.结果表明FecB突变降低了公羊和母羊的胎畜体重.BB雌性与++雌性相比,其胎畜时期卵泡形成的个体发育被延迟.BB母羊卵泡成熟的个体发育显著不同于++母羊.基因携带者小直径卵泡中,颗粒细胞含量下降,而卵泡液中cAMP含量/合成、芳香酶活性、雌二醇浓度以及颗粒细胞LH受体都增加.排卵时的卵泡直径以及黄体大小在BB个体中较小.目前的证据表明,在Booroola绵羊排卵数控制中,卵巢具有更重要的作用.     

鹌鹑暴发新城疫的诊断报告

我市某鹌鹑养殖基地养有鹌鹑143 000只,于2001年7月下旬,发生一种发病率、死亡率都很高的疾病,仅17天,就发病48 000只,发病率33.57%,累计死亡约30 000只,病死率高达62.5%,直接经济损失7.2万元.经流行病学调查,病理剖检,实验室检验,诊断为鹌鹑新城疫.基地其余95 000余只鹌鹑采取隔离、消毒、监控、紧急免疫接种,该病得到控制,现将诊断情况报道如下.     

Structural and metabolic integrity of sheep external intercostal muscle during extended culture

The objectives of this study were to examine the structural and metabolic integrity of isolated sheep external intercostal muscle bundles following variable lengths of preincubation (0 to 192 h). Samples of intact external intercostal muscle (10 to 15 g), with tendons attached, were prepared from growing wethers and maintained at their resting lengths during preincubation for 0 to 192 h. Protein synthesis (PS), protein degradation (PD), acetate oxidation and ultrastructural integrity of muscle samples were examined at 0 to 192 h, 0 to 96 h, 0 to 48 h and 0 to 96 h following isolation, respectively. Additionally, the effects of variable fetal calf serum (FCS) concentrations (0 to 20%; w/v) on PS and PD and acetate oxidation were examined. Rate of PS increased as preincubation time increased to 192 h; however, most of this increase was due to the proliferation of fibroblasts on the surface of the muscle sample. Addition of cytosine arabinoside to the incubation media prevented the fibroblast-dependent increase in PS; however, it did not entirely prevent the preincubation time-dependent increase in PS. Rate of PD increased greatly upon preincubation. The nitrogen balance of incubated muscles was negative at all times examined. Acetate oxidation was maintained through 12 h of preincubation and thereafter declined. Relatively normal myofibrillar structure was maintained through 48 h of preincubation; however, loss of mitochondrial integrity and dissolution of Z-disks at 48 h and at 96 h of preincubation were evident. Isolated tissues were able to respond to FCS concentration in medium following 48 h of preincubation.     

伊维菌素在山羊奶中残留的研究

伊维菌素是新的高效广谱驱虫剂,可防治猪、牛和羊的体内外寄生虫,但未见用药后山羊奶禁止饮用期限的规定。本实验取山羊奶5ml用异辛烷和乙腈提取,高效液相色谱法测定提取液中伊维菌素的量。色谱柱为6～8μm Zorbax-ODS(150×4.6mmID),流动相为乙腈+甲醇+2％冰乙酸水溶液(45+45+10),紫外检测波长为255nm。伊维菌素含量为4～50ng/ml时,峰面积—浓度曲线呈线性,回收率为85.0±2.8％。以300mcg/kg.b.w.剂量给山羊皮下注射伊维菌素,1～3天奶中可达峰值,含量约24ng/ml,经8天后奶中不再检出伊维菌素,试验结果表明用药后8天内的山羊奶不能供人饮用。     

应用Dot－ELISA快速检测传染性法氏囊病病毒

本文成功建立了斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ），应用于传染性法氏囊病病毒（ＩＢＤＶ）的快速检测。该方法操作简便快速、特异性好、灵敏度高。同时对深圳某鸡场发生ＩＢＤ病料进行了病毒分离，用Ｄｏｒ－ＥＬＩＳＡ检测接种鸡胚的绒毛尿囊膜裂解上清呈强阳性反应。     

论毛竹丰产林基地建设

论述了福建省龙岩市发展毛竹丰产林基地的自然优势,以及毛竹丰产林基地的形式与标准,改造现有低产毛竹林的措施,并根据不同的经营目的,论述了材用毛竹林、笋竹两用毛竹林、手工纸用毛竹林的丰产培育措施,毛竹丰产林基地的科学管理,包括机构建立,资料档案建立,竹林结构调整和病虫害防治等。