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排序方式: 共有9896条查询结果,搜索用时 46 毫秒
31.
CAI Yun-feng MIN Jun FANG Tian-ling CHU Zhong-hua DENG Xiao-geng HE Jing-song CHEN Ji-sheng 《园艺学报》2003,19(8):1029-1033
AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy. 相似文献
32.
AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth. 相似文献
33.
LING Yun-biao XU Rui-yun QIU Wan-shou CHU Zhong-hua YANG Hong-zhi TAN Zhi OU Qing-jia 《园艺学报》2003,19(12):1649-1652
AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[3H]H]proline and [[3H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P<0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P<0.05). Portal serum decreasea collagen synthesis (P<0.05), but circumferential serum had no effect (P>0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action. 相似文献
34.
‘丰悦’、‘翠玉’是从野生猕猴桃资源中选育出的优质良种, 均果大质优, 平均单果质量均为90 g 左右, 最大127. 8 g、129 g。肉质细嫩多汁, 味甜, 香气浓, 可溶性固形物含量分别达13. 5%~ 15. 8%和14. 5%~ 17. 3%, 最高达19. 0%和19. 5% , 维生素C 含量分别达840~ 1 630 和930~ 1 430 mg/ kg。果实耐贮藏, ‘翠玉’20℃下可贮藏30 d 左右。在长沙‘丰悦’9月中下旬成熟; ‘翠玉’10月上旬成熟。 相似文献
35.
36.
20世纪80年代后,稻瘿蚊发生危害明显回升,损失相当严重。以大田试验示范为主,系统研究了新耕作条件下稻瘿蚊综合防治,首次总结提出“喷药除草、压低虫源、抓住晚秧、做好测报、挑治本田”的综合防治新技术体系。室内外试验和大田示范表明,应用草甘膦杀死越冬寄主游草,可显著减少虫源基数;灭线磷(商品名益舒宝)、氯唑磷(商品名米乐尔)是防治稻瘿蚊的高效药剂,抗蚊青占品种高抗稻瘿蚊。此外,合理利用杀虫植物、性引诱剂和天敌等亦是防治稻瘿蚊的重要措施。在面积达12.46万hm^2的示范推广实践中,本田防治效果均达80%以上。 相似文献
37.
苏云金杆菌高效生产菌株的筛选 总被引:2,自引:0,他引:2
从灯蛾罹病死亡幼虫中分筛筛选出苏云金杆菌BacillusthuringiensisSB-1菌株,从引进的B.t.菌种中筛选出SB-8菌株。该两株菌经发酵试验,生产性能良好,对抗药性强的台湾、泰国和深圳品系的小菜蛾毒力较高。田间试验表明杀虫效果好。 相似文献
38.
基于GIS的中国小麦条锈病菌越夏区气候区划 总被引:16,自引:0,他引:16
基于多年的气象数据(1980~2001年),首次从制约小麦条锈病菌越夏的温度因子入手,结合寄主小麦因素,利用地理信息系统(Geographical Information System,GIS),对我国小麦条锈病菌越夏区进行较详细的气候区划。本研究从温度条件上明确了全国适合小麦条锈病菌越夏的范围。研究表明,在我国小麦种植区适合小麦条锈病菌越夏的范围很广。其中甘肃、四川、云南、陕西境内适合越夏的地区是连成一片的,甘肃东部除了西边的几个县外其它地方7、8月份最高一旬均温在20~23℃,条锈病菌越夏困难;西藏、青海境内的小麦种植区几乎都适合小麦条锈病菌越夏;贵州境内适合越夏的地区可能和云南越夏区是一个整体。云南适合越夏的地区甚广,且地形复杂,需要进一步调查研究。 相似文献
39.
40.
ZHANG Zhan-jun YING Kang WANG Zhong ZHANG Xiao-yan LIU Jian-xun HUANG Yan XU Li WEI Cui-e WANG Yong-yan 《园艺学报》2004,20(8):1427-1433
AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases. 相似文献