Genetic Transformation of Rice with Pi-d2 Gene Enhances Resistance to Rice Blast Fungus Magnaporthe oryzae

The gene Pi-d2, conferring gene-for-gene resistance to the Chinese blast strain ZB15, was isolated from a rice variety (Digu) by the map-based cloning strategy. Here, we constructed a control plasmid pZH01-pi-d2tp309 (pZH01-tp309) and three different expression constructs, pCB-Pi-d25.3kb (pCB5.3kb), pCB-Pi-d26.3kb (pCB6.3kb) and pZH01-Pi-d22.72kb (pZH01-2.72kb) of Pi-d2, driven by Pi-d2 gene's own promoter or CaMV35S promoter. These constructs were separately introduced into japonica rice varieties Lijiangxintuanhegu, Taipei 309, Nipponbare and Zhonghua 9 through Agrobacterium- mediated transformation. A total of 150 transgenic rice plants were obtained from the regenerated calli selected on hygromycin. PCR, RT-PCR and Southern-blotting assay showed that the gene of interest had been integrated into rice genome and stably inherited. Thirty-five transgenic lines independently derived from T1 progeny were inoculated with the rice blast strain ZB15. Transformants exhibited resistance to rice blast at various levels. The lesions on the transgenic plant leaves were less severe than those on the controls and the resistance level of transgenic plants harboring the gene of interest from three vectors had no difference. The own promoter of Pi-d2, about 2.2 kb or 3.2 kb, had the similar promoter function as CaMV35S. Field evaluation for three successive years supported the results of artificial trial, and some lines with high resistance to rice leaf blast and neck blast were obtained.     

冬虫夏草感染蝙蝠蛾幼虫研究进展

随着冬虫夏草资源的日趋枯竭,冬虫夏草人工繁育研究逐渐成为菌物学家和昆虫学家研究的热点,而冬虫夏草人工繁育研究的核心内容是冬虫夏草菌感染蝙蝠蛾幼虫研究.为此,就感染幼虫的龄期、感染方法、感染过程等方面进行了综述.     

对我国毛皮用绵、山羊产业发展的思考

在全面分析我国现有10个毛皮用绵、山羊品种所处生态环境和种质特性基础上,就当前存在的主要问题提出发展我国毛皮用羊的思考与建议,对我国毛皮羊的保种选育提高和更充分发挥其生产潜力具有一定的参考价值.     

Expression of SARS-CoV functional receptor angiotensin-converting enzyme 2 in nephritic epithelium of primates

AIM: To investigate the expression of angiotensin-converting enzyme 2 (ACE2) in nephritic epithelium of primates. METHODS: The expression of ACE2 in Vero E6 cells was detected by the techniques of RT-PCR and immunocytochemistry (ICC) techniques. The distribution of ACE2 protein in kidney tissues of two Rhesus monkeys and two normal human cases was observed by immunohistochemistry (IHC) techniques. RESULTS: Vero E6 cells were found to express both ACE2 mRNA and protein. ACE2 protein was mainly located in epithelium of proximal tubules of Rhesus monkey and human kidney. CONCLUSION: The expression of ACE2 in epithelium of primate kidney may provide the condition for SARS-CoV entry, which may be one of the reasons for inducing renal damage in SARS patients.     

罗汉果组培苗在湖南湘西地区的引种研究

通过对罗汉果组培苗"农青2号"进行大田种植观察分析,探讨其在湖南湘西地区的生态适应性,为推广种植罗汉果提供依据。结果表明:该品种8月上旬初花,花期110 d左右,有效授粉期25~30 d,10月下旬成熟,每667 m2产果12 986.1个,大中果率达60%,其中丰产园大中果率在80%以上,甜甙V含量0.16%,无明显病虫害,适合在湖南湘西地区推广种植。     

InDel and SNP Markers and Their Applications in Map-based Cloning of Rice Genes

High-density markers are necessary for map-based cloning of dca genes, but the currently available markers are not satisfactory enough. InDel (insertion-deletion length polymorphism) and SNP (single nucleotide polymorphism) are the new generation of molecular markers and can basically meet the need of fine mapping. InDel and SNP markers can be developed through bioinformatics. These markers are valuable markers with the characters of low cost, high specificity and stability. This article introduced the methods for designing InDel and SNP markers, taking the mapping of a dce rolled leaf gene as an example. In addition, some key factors in improving the design efficiency were also discussed.     

小型蓄冷蓄热型热泵干燥装置的理论探讨

针对在农副产品干燥加工中传统的封闭式热泵干燥装置存在的不足,介绍了一种蓄冷蓄热型热泵干燥装置,提出了蓄热时间数与蓄冷时间数的概念,以及采用控制蓄热剂温度来控制制冷压缩机的开机与停机的控制方式;并以小型装置为例,计算出采用石蜡作为蓄冷剂与蓄热剂时所需的最小用量,给出了高温中间换热器与低温中间换热器的设计。     

NAA和2，4ˉD对东方百合组织培养的影响

借助正交回归多项式建立数学模型的方法,研究了NAA对东方百合“蒂伯”（Ti ber）鳞片 诱导小鳞茎及2,4ˉD对小叶片诱导愈伤组织的影响,以探讨东方百合组织培养中NAA及 2,4ˉD 的用量范围及最佳用量。结果表明, NAA诱导离体鳞片外植体产生小鳞茎的用量范围为 0.15～ 0.9 mg·L -1 ,最佳用量为0.69 mg·L -1 ; 2,4ˉD诱导叶片形成愈伤组织的最佳用量为3.2 mg· L -1 。