Kan浓度对无菌苗生根影响的研究

在未加激素的MS培养基中添加不同浓度的Kan,研究其对桑树陕305无菌苗的生根抑制程度,确定转基因植株延迟筛选的适宜浓度。培养基中添加Kan6mg/L是延迟筛选的适宜浓度。     

燕麦在法库县种植推广的可行性探讨

介绍燕麦的营养价值及其市场前景,阐述法库县在土质、气候、地理环境、人文环境等方面种植燕麦的条件优势,通过对比种植燕麦与玉米的经济收益,探索燕麦在法库县种植的潜力。     

紫薇新品种淡爪平盘紫和大花淡爪直枝粉

淡爪平盘紫和大花淡爪直枝粉是通过广泛收集紫薇多种源、多品种的种子开展混合种子育苗,采用自然杂交实生选育法选育出的紫薇新品种。无性系遗传稳定性实验结果表明,这2个新品种遗传性状稳定、适应性广、抗逆性强、观赏价值高,是优良的园林观赏树种,在城市景观中已被推广应用。     

东河早藕土壤养分限制因子试验

通过土壤养分限制因子试验,对比了不同配方施肥条件下东河早藕田间植物学性状、生育期和经济性状的差异.试验结果表明,缺氮、磷、钾肥对东河早藕的生长发育及产量形成具有明显的抑制作用,且效果由大到小顺序为氮肥、钾肥、磷肥.针对浙中地区部分土壤缺锌,补施锌肥能促进东河早藕早熟高产.     

喜树碱对番茄灰霉病菌的病理反应

以番茄灰霉病菌为供试菌种,采用电导率法、吸光度法和电镜分析法对经过喜树碱（CPT）处理的番茄灰霉病菌进行病理反应研究。试验结果表明经200 mg/L CPT溶液处理后,番茄灰霉病菌菌丝细胞膜的通透性增加了30.7%,细胞外蛋白质浓度提高了13.5%。番茄灰霉病菌经CPT溶液处理后,还原糖和可溶性蛋白的含量随着处理时间的增长先升高后降低最后趋于平缓,在0～1 h之间增长最快。CPT处理后的番茄灰霉病菌的菌丝畸形,出现膨大或缢缩,菌丝生长点缢缩,细胞中的空腔增多,线粒体增多且线粒体嵴变得模糊,细胞核有降解的现象。     

水稻旱作试种报告

通过引种旱稻95-13-5,研究抗旱栽培水稻效果。结果表明,在盘锦新开农场,7月末至9月初灌水1～3次、灌水总量在80～150m3/667m2情况下,施行旱作栽培,可获得625～651kg/667m2产量、生产成本降低100元/667m2以上显著的经济效益。     

Endogenous hydrogen sulfide regulates oxidant/antioxidant balance in rat with chronic obstructive pulmonary disease

AIM: To investigate the role of hydrogen sulfide (H₂S) in the protection against oxidative stress in rats with chronic obstructive pulmonary disease (COPD).METHODS: The rat model of COPD was established by cigarette smoking (CS) combined with lipopolysaccharide (LPS) instillation. Thirty-two healthy male Sprague-Dawley rats were randomly divided into 4 groups: control group, CS+LPS group, CS+LPS+NaHS (H₂S donor) group and CS+LPS+PPG (DL-propargylglycine, an inhibitor of cystathionine-γ-lyase) group. After 30 days, the lung functions of the rats were measured, the histological changes of lungs were observed under light microscope and the pathological scores were calculated. The H₂S level in plasma and the protein expression of cystathionine-γ-lyase (CSE) in the lung tissues were measured. The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and catalase (CAT) were detected to reflect oxidative stress.RESULTS: Compared with control group, the peak expiratory flow (PEF) decreased by 24% and intra-pressure (IP) increased by 66% in CS+LPS group. The pathological scores of the lung tissues also increased. Compared with CS+LPS group, no change in the lung function was observed after given NaHS or PPG, but the pathological scores decreased in CS+ LPS+ NaHS group. Compared with control group, the content of H₂S in plasma was increased by 26% on day 16. Compared with CS+LPS group, the content of H₂S in plasma of CS+LPS+PPG group was decreased by 22% after 30 days. Compared with control group, the protein expression of CSE increased, and no statistical difference among CS+LPS group, CS+ LPS+ NaHS group and CS+LPS+PPG group was observed. Compared with control group, MDA content in the lung tissues was increased by 24% in CS+LPS group, the activity of SOD was increased by 47% and the activity of CAT was increased by 52%. Compared with CS+LPS group, the MDA content in CS+LPS+NaHS group was decreased by 21%, and no statistical difference in the activity of SOD and CAT was observed. The activity of SOD decreased by 33% after given PPG.CONCLUSION: H₂S plays a role as anti-oxidant in the rats with COPD. The CSE/ H₂S pathway may be involved in the development of COPD.     

零通量面法用于农田蒸发蒸腾量的研究

应用土壤水分零通量面的原理方法,对北京房山节水灌溉区进行了研究。通过土壤水势监测分析,显示了灌区在研究时段内零通量面的性质及其出现的空间位置。用数值方法处理了零通量面随时间变化的情况,计算了监测时段的蒸发蒸腾量,并与大田水量平衡模型法、作物系数-参考作物需水量法进行了比较。     

茶多酚对生长猪机体维生素E状态、抗氧化性能及肉质的影响

日粮添加维生素E对降低动物脂质过氧化具有重要作用,可以改善动物肉品质及动物健康状况。本试验在日粮中添加不同水平的茶多酚,研究其对生长猪维生素E状态、血浆抗氧化性能及肉质的影响。选择240头平均体重为(31.24±0.48)kg的三元猪,随机分为3组,每组4个重复,每个重复20头猪。试验共设计3种日粮,对照组饲喂基础日粮,处理组在基础日粮中分别添加10和100 mg/kg茶多酚,试验共进行5周。结果显示:各处理组对生长猪试验初期和末期血浆总抗氧化力和铁还原力均无显著影响(P> 0.05),但对照组、10和100 mg/kg茶多酚添加组血浆试验末期铁还原力均显著高于试验初期(P <0.05)。日粮添加茶多酚对生长猪肝脏、肌肉、血浆α-生育酚和γ-生育酚含量均无显著影响(P> 0.05),其中肝脏生育酚含量最高,之后依次是肌肉、肺和血浆。日粮添加10或100 mg/kg茶多酚,肺中α-生育酚和γ-生育酚含量较对照组有提高趋势(P=0.09)。各组对生长猪肉质均无显著影响(P> 0.05)。日粮添加100 mg/kg茶多酚较对照组提高了生长猪屠宰后48 h肌肉的滴水损失。综上所述:生长猪日粮添加茶多酚对组织维生素E水平、血浆抗氧化力及肌肉品质无显著改善作用。     

猪结合珠蛋白基因的克隆、原核表达及单克隆抗体制备

本研究的目的在于利用重组猪结合珠蛋白(Haptoglobin,Hp)制备特异性单克隆抗体.以梅山猪肝脏总RNA为模板,利用RT-PCR方法扩增猪hp基因,将其克隆到原核表达栽体pGEX-KG和pET-32a上,转化至E.coli BL21中,IPTG诱导下高效表达重组GST-Hp和HIS-Hp蛋白.用纯化的重组GST-Hp免疫BALB/c小鼠,用纯化的HIS-Hp作为包被抗原进行间接ELISA检测,通过淋巴细胞杂交瘤技术筛选阳性细胞株,制备单克隆抗体.结果表明筛选到2株高效分泌抗猪Hp单克隆抗体的杂交瘤细胞株,命名为3B4和3C8.Western-blot和间接ELISA检测均表明制备的单克隆抗体具有良好的特异性.