反相高效液相色谱法测定板栗中多菌灵残留量

建立了反相液相色谱法测定板栗中多菌灵残留量的分析方法:在酸性溶液中,以甲醇作为提取剂,石油醚去除脂类杂质,二氯甲烷进行萃取提纯,色谱柱为Kromasil C18(250 mm×4.6 mm,5μm)不锈钢柱,流动相为V(甲醇)/V(0.02 M乙酸铵,pH8)=32/68,柱温38℃,流速1.0 mL/min,紫外检测波长285 nm,以外标法定量,实验证明,检测线性范围0.05～5.00 mg/L,相关系数r=0.999 9,板栗中多菌灵添加回收率94.4%～103.0%,变异系数≤2.3%,最低检出量为1.0x10-9g(S/N=3),检出精度为0.01 mg/kg.     

地形对梭梭立地条件的影响Ⅰ:坡位

选取了坡度为30°半固定沙丘坡面上不同坡位的梭梭为研究对象,初步分析了坡位与土壤养分、盐分分布特征的关系及梭梭对土壤理化性质的影响.结果表明,有梭梭条件下,土壤有机质,速效氮、磷、钾、总盐含量在不同坡位的高低顺序为:坡顶＜坡面＜坡底,表明了土壤养分及可溶性盐分具有坡底富集的特征;有梭梭土层的养分、盐分含量均高于无梭梭对照土层,表明梭梭对土壤养分、盐分有一定的富集作用.     

民勤绿洲人工治沙林自然更新能力调查研究

民勤绿洲治沙造林以梭梭、柠条、沙拐枣为主要树种,经多年定点观测研究,结果表明:梭梭、柠条、沙拐枣在民勤地区均能实现一定量的自然更新,更新量与当年降水量及降水的理想分布呈正相关;梭梭更新一般发生在春季,但春夏无有效降水的情况下,秋季也有少量更新,而柠条、沙拐枣的更新以秋季为主,翌年春季也有更新;3树种自然更新对林木盖度增加几无影响,6年间梭梭、柠条、沙拐枣每hm2株数分别增加了16.7%、8.0%、37.2%;3树种自然更新有明显的地域差异性;个例调查,沙拐枣良好更新具有普遍性,柠条次之,而梭梭只能为个例;更新能力沙拐枣柠条梭梭。     

固相微萃取技术在食用植物油分析中的应用

介绍了固相微萃取(SPME)技术的装置device、原理、萃取模式、影响因素、技术特点和应用范围;概述了SPME技术在植物油溶剂残留、香气组分分析、污染物的测定以及评估植物油氧化程度或氧化稳定性中的应用.     

利用CRISPR/Cas9敲除葡萄VviPDS1基因的研究

选择葡萄八氢番茄红素脱氢酶(Phytoene desaturase)基因VviPDS1为靶标,利用CRISPR/Cas9系统构建基因敲除载体,瞬时转化葡萄叶片原生质体,检测到不同类型的突变。通过农杆菌介导转化‘无核白’葡萄胚性愈伤组织,筛选获得卡那霉素抗性植株71株。经PCR鉴定,其中53株为阳性植株,阳性率为74.64%。测序结果表明,共有20株在靶点发生不同类型的突变,编辑效率为37.74%;其中9株产生了双等位基因突变。对其进行氨基酸序列预测,在第202位氨基酸之后发生了不同程度的变异。利用CRISPR/Cas9系统敲除VviPDS1获得的突变体植株呈现整体矮化,其叶片出现不同程度白化。表明CRISPR/Cas9系统可以通过细胞中的瞬时或稳定表达进行基因编辑,可以实现在葡萄编辑植株中产生纯合敲除。     

Research progress on autophagy in treatment of glioma

Autophagy is a transport pathway from the cytoplasm to the lysosome, which is a major intracellular degradation/recycling system ubiquitous in eukaryotic cells. Autophagy regulation has achieved some gratifying results in the treatment of glioma. It is currently an exciting field of clinical development. In chemotherapy or radiotherapy, autophagy-related drugs are currently used in vitro and in vivo for treating tumors with significant effects. Autophagy inducers and inhibitors may potentially block tumor formation and enhance the anti-cancer immune response. A more comprehensive understanding of the role of autophagy in different stages of glioma development may guide the development of new therapeutic strategies.     

瑞丽山龙眼幼苗光合特性研究

使用Li-6400XT便携式光合仪测定高黎贡山南段2种环境(野外、大棚)下生长的我国特有种植物瑞丽山龙眼幼苗的光合特性,结果表明:(1)2种环境下生长的幼苗净光合速率(PN)日变化出现"双峰"曲线,有"午休"现象,但峰值出现的时刻与大小不同,野外幼苗在10:00、16:00出现高峰值,分别为4.608、2.118μmol/(m~2·s),大棚幼苗在11:00、15:00出现2个高峰值,分别为4.102、3.982μmol/(m~2·s);蒸腾速率(Tr)、气孔导度(Gs)变化趋势与净光合速率变化趋势一致,也有"午休"现象;水分利用率(WUE)无明显变化规律;胞间CO_2浓度(Ci)与净光合速率日变化相反。(2)净光合速率与环境因子偏相关性分析表明,环境因子:光合有效辐射(PAR)、大气CO_2浓度(Ca)、空气温度(Ta)、相对湿度(RH)对幼苗PN值影响均为:PARCaTaRH;(3)两地幼苗光饱和点(LSP)均较高,野外为2 674.995μmol/(m~2·s)、大棚幼苗为2 228.754μmol/(m~2·s),光补偿点(LCP)分别为38.250、8.322μmol/(m~2·s)。     

抗除草剂谷子新品种赤谷20的选育及简化栽培技术

利用夏谷区谷子抗除草剂基因,选育适合简化栽培的两系谷子新品种赤谷20.该新品种由抗除草剂拿捕净的新品系k 23-18及不抗除草剂的同型姐妹系k 23-19组成.2014-2015年参加全国谷子品种区域试验,平均单产6 292.50 kg/hm2,较对照谷子品种九谷11(平均单产5 914.5 kg/hm2)增产6.39％.2015年12月通过全国谷子品种鉴定委员会鉴定.通过对辅助除草“剂谷有”用药的研究、对同型姐妹系谷种的最佳混合比例、播种时期、播种量的研究以及对除草剂拿捕净最适宜的喷药时期、喷药浓度、安全剂量等方面研究,制定与其配套的简化栽培技术.确定了同时实现间苗和除草的化学方法,实现全生育期基本不用人工间苗与除草,简化劳动力.     

Effects of CENP-W down-regulation on human glioma U87 cells

AIM: To study the effect of centromere protein W (CENP-W) down-regulation on human glioma U87 cells.METHODS: Small interfering RNA (siRNA) was used to inhibit the expression of CENP-W in the U87 cells. The interference effect of siRNA was evaluated by RT-qPCR and Western blot. The proliferation of the cells was analyzed by MTT assay, BrdU staining and colony formation experiment. Transwell chamber assay was used to detect the invasion ability of the cells. The cell migration ability was measured by a scratch test. The changes of the cell cycle distribution and apoptosis were analyzed by flow cytometry.RESULTS: The results of MTT assay, colony formation experiment and BrdU staining showed that the cell proliferation and colony formation abilities in experimental group were significantly lower than those in control group and negative control group. The results of Transwell and scratch experiments showed that the migration and invasion abilities in experimental group were weaker than those in blank control group and negative control group. The results of flow cytometry analysis showed that the cell cycle distribution in experimental group was arrested in G₀/G₁ phase. The percentage of apoptotic cells in experimental group was higher than that in control group (P<0.05).CONCLUSION: Down-regulation of CENP-W expression inhibits the proliferation, migration and invasion of human glioma cells and promotes the apoptosis of the cells, suggesting that CENP-W may be a potential target of gene therapy for human glioma.