Mung bean trypsin inhibitor is effective in suppressing the degradation of myofibrillar proteins in the skeletal muscle of blue scad (Decapterus maruadsi)

Mung bean trypsin inhibitor (MBTI) of the Bowman-Birk family was purified to homogeneity with a molecular mass of approximately 9 kDa on tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 8887.25 Da as determined by matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight mass spectrometry (MALDI-QIT-TOF MS). Using blue scad myofibrillar proteins as targets, it was found that, in the absence of MBTI, proteolysis of myofibrillar proteins, especially myosin heavy chain (MHC), could be identified after incubation at 55 °C for 2 h, while in the presence of MBTI, with a final concentration of 25 ng/mL, proteolysis of these proteins was greatly suppressed even after incubation for 3 h. Although cysteine proteinase inhibitor E-64 was also effective in preventing protein degradation, inhibitors for metallo- and asparatic proteinases did not reveal obvious inhibitory effects. Our present results strongly suggested that the naturally occurring legume bean seed protein MBTI can be used as an effective additive in preventing marine fish blue scad surimi gel softening, which is quite possibly caused by myofibril-bound serine proteinase (MBSP).     

Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis

Agarose is a natural seaweed polysaccharide and widely used in the medicine, food, and biological fields because of its high gel strength, non-toxicity, and electrical neutrality. The sulfate group is one of the main charged groups that affect the performance of agarose. In the present study, a simple, eco-friendly, and efficient method was explored for agarose preparation. After desulfation with hydrogen peroxide (H₂O₂), the sulfate content of agar reached 0.21%. Together with gel strength, electroendosmosis, gelling and melting temperature, the indicators of desulfated agar met the standards of commercially available agarose. Notably, the desulfated agar can be used as an agarose gel electrophoresis medium to separate DNA molecules, and the separation effect is as good as that of commercially available agarose. Further, the H₂O₂ desulfation process was analyzed. The addition of a hydroxyl radical (HO•) scavenger remarkably decreased the H₂O₂ desulfation rate, indicating that HO• has a certain role in agar desulfation. Sulfate content detection indicated that sulfur was removed from agar molecules in the form of sulfate ions (SO₄²⁻) and metal sulfate. The band absence at 850 cm⁻¹ indicated that the sulfate groups at C-4 of D-galactose in sulfated galactan were eliminated.     

建筑模板用竹编胶合板生产工艺研究

建筑模板用竹编胶合板系用毛竹经劈篾、编织、涂胶组坯、热压而成的竹材人造板。在生产中使用的半成品其含水率应控制在5～8％,胶粘剂在涂刷前需进行P型柔韧剂的改性和A型缓冲剂的调配,胶粘剂的用量为:竹席双面300～350g/cm~2,竹帘双面250～300g/m~2。热压工艺采用三段式,即低压保压——高压保压——降压保压,低压段在5～10 kg/cm~2单位压力下保持1～2 min,高压段在30～50 kg/cm~2单位压力下保持3～8 min,降压段在3～6kg/cm~2保持2～3 min。热压温度为130℃左右。     

胚胎移植技术在优质细毛羊育种中的研究与应用

采用 FSH、L H、PMSG、氟孕酮阴道海绵栓等激素进行超数排卵 ,通过人工授精、手术采卵、同期发情、胚胎移植、B超妊娠检查等技术 ,共处理优秀美利奴母羊 4 5 6只 ,获得可用胚胎 (A、B、C级胚胎 ) 30 33枚 ,平均 6 .6 5枚 /只(30 33/ 4 5 6 ) ,移植受体母羊 2 333只 ,冻胚受孕率 4 7.0 2 % (10 97/ 2 333) ,产羔数 14 2 6只 ,产羔率 12 9.99% (14 2 6 /10 97)。其中 ,公羔 6 85只 ,母羔 74 1只。优质细毛羊初生重公羔平均 4 .4 5 kg,母羔平均 4 .6 1kg。断奶重公羔平均38.2 4 kg,母羔平均 33.6 8kg。 12月龄平均体重公羊 76 .15 kg,母羊平均 6 2 .15 kg。周岁毛长公羊平均 11.6 7cm,剪毛量平均 12 .0 4 kg;母羊毛长平均 11.12 cm ,剪毛量平均 7.6 5 kg;平均净毛率达到 6 8.5 %。 2岁公羊体重平均 10 6kg,毛长平均 11.5 cm,剪毛量平均 11.85 kg,净毛率 6 9.5 %。2岁母羊体重平均 72 kg,毛长平均 11.0 cm,剪毛量平均8.5 kg,净毛率 6 9.0 6 %。羊毛纤维平均细度 18.71μm。 3岁公羊最大体重 14 8kg,母羊最大体重 10 2 kg。优质细毛羊新类群同期上述各项指标均显著或极显著高于中国美利奴羊     

播量和行距对紫羊茅种子产量的影响

本研究是在呼和浩特市的自然条件下，对紫羊茅种子产量在不同播种量和不同行距情况下进行了试验。结果表明，紫羊茅的最适播量７．５０ｋｇ／ｈｍ２，行距为３０ｃｍ，在此播种体系中种子产量平均可达６６２．５ｋｇ／ｈｍ２。     

Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat

The greenbug, Schizaphis graminum (Rondani), is the most economically damaging aphid pest of wheat in the southern Great Plains of the USA. In this study, the single, dominant greenbug resistance gene, Gb3, was molecularly tagged and genetically mapped using amplified fragment length polymorphism (AFLP) and simple sequence repeat(SSR) markers. Three AFLP loci were associated with the Gb3 locus in linkage analysis with 75 F_2:3 families from the cross between two near‐isogenic lines (NILs) for Gb3,‘TXGBE273’ and ‘TXGBE281′. Two of these loci, XMgcc Pagg and Xmagg Patg cosegregate with Gb3 in the population analysed. Further analysis indicated that XMgcc Pagg and Xmagg Patg are specific for the Gb3 locus in diverse genetic backgrounds. Two SSR markers, Xgwm111 and Xgwm428 previously mapped in wheat chromosome 7D, were shown to be linked with Gb3, 22.5 cM and 33.1 cM from Gb3, respectively, in an F₂ population of ‘Largo’בTAM 107’, suggesting that Gb3 is located in the long arm of chromosome 7D. The two AFLP markers cosegregating with Gb3 are valuable tools in developing molecular markers for marker‐assisted selection of greenbug resistance in wheat breeding.     

论柳树家族及其在园林绿化上的应用

柳树资源丰富,适应性广,观赏价值较高,耐水湿,在中国城乡绿化中具有悠久的利用历史。它不仅在农业、园林等方面有着广泛的应用,而且在中国古代还形成了独特的“柳文化”。笔者依据相关文献,概述了具有较高应用价值的柳树家族种类的生物学特性、生态习性及主要观赏特性,总结了柳树的文化内涵,阐述了柳树的综合价值以及园林绿化应用形式,指出了柳树树种资源在园林应用方面存在的问题,并提出了对策。     

水稻白叶枯病抗性基因的聚合及其遗传效应

以全生育期抗水稻白叶枯病的早粳Ｇ３８为抗源供体，以成株期抗性的晚粳品种秀水１１为轮回亲本，通过连续回交转育出全生育期抗白叶枯病的晚粳品系Ｄ６０１、Ｄ６０２和Ｄ６０３。遗传研究表明，３个晚粳品系都聚合了来自双亲的ｘａ－５和Ｘａ－３抗性基因，聚合的抗性基因可能具有抗性累加效应，其抗性水平和抗扩展能力强于双亲，抗谱宽于秀水１１。同时评价了３个晚粳稻抗病品系对我甸白叶枯病病原型的抗性及主要农艺性状。认为Ｄ     

旱地玉米双相覆盖技术在太原地区的增产效果与机理

玉米双相覆盖技术在太原生态区增产率为１４．４８％。具有苗期适宜增温促发、中期保墒促长的土壤环境效应。增产机理是：玉米地上部和地下部协调增长，叶面积大，茎秆还原糖贮量多，干物质积累也多；具持续高强度灌浆特性，加快了物质运转速度