昆明鼠卵母细胞的孤雌发育及对其干扰核移植试验的预防措施

本文着重研究了在昆明鼠细胞核移植过程中，卵母细胞孤雌发育的激活原因、形成过程，并提出了一些防止其干扰细胞核移植的措施。     

Epidermal growth factor-like activity in mares' milk.

Epidermal growth factor (EGF)-like activity was measured in mares' colostrum and milk by radioreceptor assay. Milk samples were collected from 22 mares 1 or more times during early lactation. Samples of colostrum were taken after parturition and before the foal first suckled (presuckle), within 6 hours after the foal first suckled (postsuckle), and on days 1, 2, 4, and 8 of lactation. In the 5 mares from which milk samples were obtained at each sampling time, presuckle colostral mean EGF-like activity (17.8 ng/ml) was greatest (P less than 0.05). The mean values for EGF-like activity at all other sampling times were not significantly different from each other (postsuckle colostrum, 9.7 ng/ml; day 1, 9.6 ng/ml; day 2, 8.5 ng/ml; day 4, 8.0 ng/ml; day 8, 7.8 ng/ml).     

Long-term study of aerobic bacteria of the genital tract in stud dogs.

The aerobic bacterial flora of the genital tract was characterized in 15 stud dogs in an 18-month study. The dogs represented 4 breeds and were from 3 kennels. Bacterial samples from the prepuce and semen were collected every month, except in connection with matings, when they were collected weekly (464 samples). The dogs that were included all mated at least once during the study. The mean pregnancy rate, litter size, and pup mortality for the bitches with which they had mated were all within normal limits. The most frequent bacteria isolated from the prepuce and semen were Pasteurella multocida, beta-hemolytic streptococci, and Escherichia coli. There was a tendency for breeds to differ in frequency of the most common bacterial species. Bacterial culture yielded no aerobic growth in 14.2% of the preputial samples and 69.8% of the semen samples. Bacteria were transferred between dog and bitch at mating. In this study of healthy breeding dogs, neither the fertility of the dog nor that of the bitch was affected by the bacteria transferred.     

Clinical response of nasal adenocarcinoma to cisplatin chemotherapy in 11 dogs.

In 11 dogs with nasal adenocarcinoma, IV treatment with 2 to 8 cycles of cisplatin at a dosage of 60 mg/m2 of body surface, given at 3-week intervals, resulted in a survival time of 8 to 128 weeks (mean, 32.7 weeks; median, 20 weeks). Radiography revealed complete remission in 2 dogs and partial remission in 1 dog, for an overall response rate of 27%. Clinical problems such as nasal discharge (n = 11), sneezing (n = 6), and epistaxis (n = 4) resolved in 1 to 2 weeks in all dogs after beginning chemotherapy.     

Study of prostatic disease in dogs: 177 cases (1981-1986).

Historical and physical signs associated with prostatic disease diagnosed in dogs over a 5.5-year period were defined. One hundred seventy-seven male dogs were determined to have prostatic abnormality. Of the 177 dogs, 87 were determined to have specific prostatic disease. The most common prostatic disease identified in this study was bacterial prostatitis, followed by prostatic cyst, prostatic adenocarcinoma, and benign hyperplasia. The most common prostatic disease identified in neutered dogs was prostatic adenocarcinoma. Mean age at onset of prostatic disease was 8.9 years; statistically significant difference was not observed between age at onset of the various types of prostatic disease identified. Doberman Pinscher was the most common breed with prostate disease. Twenty-nine percent of dogs with a specifically identifiable prostatic disease had signs of systemic illness, 41% had signs of lower urinary tract disease, 28% had signs of gastrointestinal tract abnormalities, and 13% had signs of locomotor difficulty.     

Effect of a supply of raw or extruded rapeseeds on digestion in dairy cows.

The influence of extruded oilseeds on total tract digestibility and ruminal digestion in dairy cows was studied in three cows fed a hay-concentrate (60.5/39.5; 3.7% fatty acids in diet on DM basis) control diet (C) or the same diet supplemented with raw (R) or extruded (ER) rapeseeds (8.0% fatty acids in diet DM). The experimental design was a 3 x 3 Latin square design. Compared with diet C, diets containing rapeseed decreased ruminal OM digestibility (9.5%, P less than .10) and increased (P less than .05) the proportion of propionate in ruminal fluid VFA. Extrusion had no effect on DM and OM total tract digestibilities and increased (P less than .10) N digestion. Microbial N flow at the duodenum was calculated taking into account solid-adherent bacteria (SAB) and liquid-associated bacteria (LAB). Duodenal flows of total, SAB, and LAB of OM and N did not change with diet. Extrusion of the rapeseeds did not modify (P less than .10) the proportion of bacterial N at the duodenum and had no effect on crude fiber digestibility. This trial demonstrates that rapeseeds in hay-based diets can be fed at levels of up to 14% of the diet without adversely affecting crude fiber digestibility.     

Evaluation of the Hennessy grading probe to predict yields of lamb carcasses fabricated to multiple end points.

Lamb carcasses (n = 278) were selected immediately after slaughter and fat thickness was measured with the SP2 Hennessy grading probe (HP) at the interface of the 12th and 13th ribs, 3.8 cm from the backbone. After a 24-h chilling period, carcasses were graded by a USDA grader and probed with the HP to obtain a fat thickness measure on the chilled carcass. One hundred sixty-five carcasses were fabricated into wholesale cuts (.64 cm of external fat trim), and 113 carcasses were fabricated into tray-ready retail cuts (.25 cm of external fat trim). Carcass weight, fat thickness (metal probe), adjusted fat thickness, hot and chilled carcass HP fat measures, as well as kidney and pelvic fat percentage and USDA yield grade, were highly correlated to cutting yield for both fabrication methods. Regression models developed to predict wholesale cut yields using HP or grader-collected measures were similar with respect to predictive accuracy. Fat thickness explained most of the variation in wholesale and tray-ready cut yields among the variables collected by the grader. Kidney and pelvic fat accounted for more of the variation in yield of wholesale cuts during stepwise regression to determine HP equations, but for predicting tray-ready yields, fat thickness taken with the HP accounted for the largest amount of variation. Equations developed to predict tray-ready retail cut yields using the HP or USDA grader-collected carcass measures were similar in the amount of variation explained. Kidney and pelvic fat percentage must be included in equations to maximize predictive accuracy when this depot site is left in carcasses.     

Cryptococcal osteomyelitis in a dog

Osteomyelitis caused by Cryptococcus neoformans was diagnosed in a three-year-old male Irish wolfhound. The case details and radiological findings of radiolucency and periosteal reaction of the left third metatarsal bone had lead to an initial diagnosis of a malignant bone tumour. Biopsy revealed a fungal granuloma and culture identified the causal organism. A four month course of ketaconazole was instigated and the lameness resolved. Twelve months after the end of treatment there was no recurrence of the problem.     

Consistency of results from the Vettest 8008 clinical biochemistry analyser.

Findings are presented on the precision of a clinical biochemistry analyser designed specifically for veterinary use. Twenty biochemical analytes have been examined in detail for variation within and between runs. The results indicate that the analyser can provide high precision for all the analytes with the possible exception of calcium, and suggest that the instrument can be used with confidence in the practice laboratory to aid diagnosis and to monitor biochemical changes in animals receiving treatment.