An outbreak of malignant catarrhal fever in Père David's deer (Elaphurus davidianus)

Thirteen (54%) of 24 Père David's deer (Eluphurus duvidianus) imported from England died of malignant catarrhal fever (MCF) within a year of arrival at Invermay Agricultural Centre. The duration of clinical illness was from one to approximately 33 days, and the most common clinical signs were depression, a hunched stance and diarrhoea. On post-mortem examination, most deer showed recent localised haemorrhages, often into the large intestine. In all deer there was histological vasculitis especially in the brain.     

Blood thiamine levels in normal cattle and sheep at pasture

Analysis of whole blood samples from 174 cattle and, 174 sheep from 3 geographical regions of New Zealand over a 10 month period showed a mean (range) thiamine level of 122 nmol/l (71-237 nmol/l) for cattle and 118 nmol/l (67-227 nmol/l) for sheep. Regional and seasonal differences were noted with levels tending to rise over the summer period. A reference range of 75-185 nmol/l is proposed for both cattle and sheep to cover these variations. Levels below 50 nmol/l are considered indicative of deficiency.     

Observations on the use of feeding space in a commercial broiler chicken house

1. Observations were made on the use of food pans and food trays by commercially grown broiler chickens.

2. Birds fed by going inside the feeders on top of the food or from standing around the outside of the feeders. The feeding times of birds inside feeders were significantly longer than those of birds outside. Different reasons to account for this are proposed for the 2 types of feeder.

3. The total number of birds able to feed together from a tray or pan changed as the birds grew. With trays this change in the number of birds was a steady decline. With pans the change was non‐linear and reflected a changing ratio of birds eating in to birds eating out. This ratio was influenced by pan design features and by competition for feeding space.

4. The data suggest that a linear measure of feeding space, as used in research trials and Codes of Practice for Welfare, does not adequately describe birds’ access to and use of feeding space. Implications for both research and the commercial industry are discussed.     

Corneal sequestrum in the cat. Repair using a tarso-conjunctival pedicle flap

Corneal sequestrum was diagnosed upon clinical examination of 12 cats. The cornea of seven were examined histologically and had focal necrosis and inflammation of the stroma and epithelium. As the simple excision of the corneal lesions had been unsuccessful in two of the cats, repair of these was effected by construction of a tarso-conjunctival pedicle flap after the excision of the sequestrum.     

Platelet activating factor as a mediator of equine cell locomotion

Equine polymorphonuclear (PMN) and mononuclear (MN) leucocytes were separated on Percoll gradients and used to study the chemoattractant properties of the polar ether-linked phospholipid, platelet activating factor (PAF). Six concentrations of PAF ranging from 1 ng/ml to 100 g/ml were studied in each of two in vitro assay systems, the agarose microdroplet and a microfilter technique. Very significant (p<0.01) increases in the movement of both PMN and MN cells were obtained with most concentrations of PAF. In two instances there was no apparent concentration-response relationship, although the action of PAF was approximately bell-shaped in two others. The possible significance of these findings for equine inflammatory conditions is discussed.     

Escherichia coli sepsis and endotoxemia in conscious young pigs

In conscious pigs the influence of intravenous infusion of live E. coli (7×10⁸/kg), of the equivalent amount of endotoxin (20 g/kg) or of a high dose of endotoxin (2.5 mg/kg) on the hemodynamic, clinical and pathological parameters and on survival rate was studied. E. coli and endotoxin infusion resulted in pulmonary hypertension, systemic arterial hypotension, a decrease in cardiac output and an increase in heart rate. Clinical signs were characterized by respiratory and nervous disturbances, whereas necropsy revealed hemorrhages and edema in several organs. Although these findings were similar in the three groups, a marked difference in lethality was observed. Infusion of E. coli or of the high dose of endotoxin resulted in a significant mortality, whereas all pigs survived the infusion of the low dose of endotoxin. This suggests that the lethal pathophysiological mechanisms may only become activated when a sufficient amount of endotoxin is released into the circulation.     

Immunophenotype of pulmonary cellular infiltrates in sheep with visceral caseous lymphadenitis

Pulmonary lesions associated with Corynebacterium ovis were analyzed with an indirect immunoperoxidase staining technique using monoclonal antibodies. The predominant cells in abscess walls and surrounding lung parenchyma were large macrophages which expressed major histocompatibility complex (MHC) class II molecules on their surfaces. T lymphocytes were prominent in the same sites in the naturally occurring lesions, and SBU-T4-positive ("helper/inducer") cells were the major subset of lymphocytes (mean T4/T8 ratio = 3.5). B lymphocytes and granulocytes comprised minor populations of infiltrating cells. These results implicate activated macrophages and MHC class II-restricted T lymphocytes in the pathogenesis of established C. ovis infections in sheep.