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91.
Introduction of new traits into cotton through genetic engineering: insect resistance as example 总被引:1,自引:0,他引:1
C. Pannetier M. Giband P. Couzi V. Le Tan M. Mazier J. Tourneur B. Hau 《Euphytica》1997,96(1):163-166
The main goal of gene transfer into cotton is the development of insect-resistant varieties. The stakes are important since
cotton protection against insects uses almost 24% of the world's chemical insecticides market, which is not without consequences
on the environment. The first approach was to introduce and express in the cotton genome, genes from the bacterium Bacillus
thuringiensis (B.t.) which produces entomopathogenic toxins. The development of an efficient Agrobacterium tumefaciens mediated
transformation system was the first step. The expression of B.t. genes was studied and synthetic genes more adapted to a plant
genome have been constructed. Studies on their expression in cotton is underway. The second focus was to develop strategies
that would minimize the risks of inducing insect resistance. The main approach is to associate several genes coding for entomopathogenic
proteins with different modes of action. Genes encoding protease inhibitors were chosen. One possibility is to associate a
B.t. gene and a gene encoding a protease inhibitor. Several protease inhibitors were tested in artificial diets on major pests
of cotton. The corresponding genes have been introduced into the cotton genome. These various orientations of the research
program will be presented.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
92.
K L Podoll J B Bernard D E Ullrey S R DeBar P K Ku W T Magee 《Journal of animal science》1992,70(6):1965-1970
Food and Drug Administration regulations currently permit addition of .3 mg of Se per kilogram of diet for chickens, turkeys, ducks, swine, sheep, and cattle. However, field reports indicate that this level may not be adequate for ruminants in all situations. Because sodium selenite is the most common supplemental form and is known to be readily absorbed to particles or reduced to insoluble elemental Se or selenides in acid, anaerobic environments, studies were conducted with dairy cattle, sheep, and horses fed sodium selenate to determine whether Se from this source was more bioavailable than Se from sodium selenite. A 2-wk period of no Se supplementation was followed by 49 or 56 d of Se supplementation at .3 mg/kg of dietary DM. Serum Se concentrations and glutathione peroxidase (GSHPx) activities measured initially and periodically thereafter revealed no difference between Se forms in sheep and horses and only a small (P less than .05) advantage for selenate in supporting serum Se concentration in dairy cattle. Selenium concentrations in skeletal muscle and liver of sheep were not different between Se forms. Serum Se, but not GSHPx, increased with time, and .3 mg of supplemental Se per kilogram of dietary DM from either sodium selenate or sodium selenite supported normal serum Se concentrations in sheep, dairy cattle, and horses. 相似文献
93.
94.
J M Aynaud S Bernard E Bottreau I Lantier H Salmon P Vannier 《Veterinary microbiology》1991,26(3):227-239
A transmissible gastroenteritis (TGE) coronavirus mutant (188-SG), selected as attenuated and resistant to acidity and proteases of the digestive tract of adult pigs, was used as vaccine ("Nouzilly strain") in sows to protect suckling piglets against a challenge exposure carried out with a highly virulent TGEV strain. The pregnant sows were immunized once (42-49 days before farrowing) or twice (42-49 and 7-15 days before farrowing) by the oral, intramuscular or conjunctival route with the 188-SG strain. Sows exposed to virulent TGEV in the field and experimentally infected sows (two oral inoculations during pregnancy) were used as positive controls leading to high protection. The neutralizing antibody response to vaccination and/or infection was studied in serum and milk. No protection against mortality was observed in the litters of (1) the nine seronegative, susceptible sows, with piglet mortality of 65/70, (2) the seven once orally vaccinated sows, with mortality of 44/54, (3) the seven sows vaccinated twice by the conjunctival route, with mortality of 55/76. Moderate protection was observed in (1) the eight sows vaccinated intramuscularly twice with piglet mortality of 36/90, (2) the seven orally and intramuscularly vaccinated sows with piglet mortality of 31/51. In of 3 contrast, improved protection was observed in (1) the 10 sows vaccinated twice orally, with piglet mortality of 23/95, (2) the four naturally infected sows with piglet mortality of 6/41, (3) the six sows experimentally infected with virulent TGEV with piglet mortality of 1/59. No correlation was found between neutralizing antibodies titers in serum and milk and protection rate of the piglets. The results indicate that relative protective lactogenic immunity against TGEV is induced only by repeated ingestion of the attenuated 188-SG strain of TGEV. 相似文献
95.
96.
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98.
Efficacy and Toxicity of Doxorubicin/Cyclophosphamide Maintenance Therapy in Dogs with Multicentric Lymphosarcoma 总被引:1,自引:1,他引:0
G. Sylvester Price DVM Rodney L. Page MS DVM Bernard M. Fischer DVM Jay F. Levine DVM MPH Thomas M. Gerig PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1991,5(5):259-262
Doxorubicin/cyclophosphamide were evaluated as maintenance drugs for dogs with multicentric lymphosarcoma (n = 28). Median remission time of all dogs was 173 days. Remission duration was shorter, however, in dogs with stage IV/V disease, in dogs with pretreatment hypoalbuminemia, and in dogs that had received glucocorticoids before initiation of chemotherapy (P less than 0.04). Nineteen dogs were evaluable for toxicity. Dose-limiting gastrointestinal toxicosis was observed in three dogs, neutropenia was observed in three dogs, and cardiomyopathy was observed in three dogs. The doxorubicin/cyclophosphamide protocol described in this report is safe and effective in treating canine multicentric lymphosarcoma. Clinical stage, pretreatment steroid therapy, and hypoalbuminemia are prognostic factors for response to this protocol. 相似文献
99.
During 1981, an experiment was conducted to examine the spatial variability of surface temperature within differentially irrigated grain sorghum fields. The measurements were performed at the University of California-Davis in 85 m long transects measured with an infrared thermometer reading at 1.0 m intervals. These measurements were made repeatedly throughout the season. When the data were analyzed to evaluate the spatial variability along each transect, it was found that along the transects the readings at 1 m intervals were not correlated. This lack of structure or spatial dependence within a given field suggests that a random sampling in a field would be adequate for infrared thermometer measurements. During a drying cycle from one irrigation to the next, the variance of surface temperature along a transect increased. However, there was not a statistically significant relationship between the amount of water extracted from the upper 150 cm of the profile when more than 40% of the available water remained. When more than 60% of the available water had been removed, the variance increased above 0.5°C2 but below this amount the variances did not exhibit any significant pattern. These data suggest that the variance of surface temperature within a field may not be a sensitive indicator of the soil water status. Utilizing the fact that the surface temperatures along the transects were random and normally distributed, we calculated that the number of samples needed to achieve a confidence limit of 1.0°C about the mean was 10 samples for a field with more than 40% available soil water and increased to 20 samples as the field dried below the 60% extraction level. These data suggest that the spatial variability techniques could be utilized to evaluate agricultural fields for their distribution efficiencies and problems. In application the samples required per field are a manageable number and could be analyzed with classical statistical methods. 相似文献
100.