全文获取类型
收费全文 | 60303篇 |
免费 | 3417篇 |
国内免费 | 36篇 |
专业分类
林业 | 2425篇 |
农学 | 1851篇 |
基础科学 | 301篇 |
6616篇 | |
综合类 | 10220篇 |
农作物 | 2087篇 |
水产渔业 | 2798篇 |
畜牧兽医 | 33009篇 |
园艺 | 705篇 |
植物保护 | 3744篇 |
出版年
2018年 | 895篇 |
2017年 | 912篇 |
2016年 | 808篇 |
2015年 | 773篇 |
2014年 | 839篇 |
2013年 | 2575篇 |
2012年 | 1669篇 |
2011年 | 2019篇 |
2010年 | 1269篇 |
2009年 | 1250篇 |
2008年 | 1879篇 |
2007年 | 1846篇 |
2006年 | 1727篇 |
2005年 | 1580篇 |
2004年 | 1501篇 |
2003年 | 1543篇 |
2002年 | 1470篇 |
2001年 | 1809篇 |
2000年 | 1783篇 |
1999年 | 1462篇 |
1998年 | 620篇 |
1997年 | 606篇 |
1996年 | 546篇 |
1995年 | 641篇 |
1994年 | 600篇 |
1993年 | 571篇 |
1992年 | 1221篇 |
1991年 | 1369篇 |
1990年 | 1258篇 |
1989年 | 1265篇 |
1988年 | 1163篇 |
1987年 | 1136篇 |
1986年 | 1194篇 |
1985年 | 1194篇 |
1984年 | 960篇 |
1983年 | 900篇 |
1982年 | 582篇 |
1979年 | 891篇 |
1978年 | 701篇 |
1977年 | 694篇 |
1976年 | 711篇 |
1975年 | 714篇 |
1974年 | 866篇 |
1973年 | 842篇 |
1972年 | 812篇 |
1971年 | 727篇 |
1970年 | 769篇 |
1969年 | 733篇 |
1968年 | 650篇 |
1967年 | 656篇 |
排序方式: 共有10000条查询结果,搜索用时 21 毫秒
991.
Lillehaug A Bergsjø B Schau J Bruheim T Vikøren T Handeland K 《Acta veterinaria Scandinavica》2005,46(1-2):23-32
Faecal samples were collected, as part of the National Health Surveillance Program for Cervids (HOP) in Norway, from wild red deer, roe deer, moose and reindeer during ordinary hunting seasons from 2001 to 2003. Samples from a total of 618 animals were examined for verocytotoxic E. coli (VTEC); 611 animals for Salmonella and 324 animals for Campylobacter. A total of 50 samples were cultivated from each cervid species in order to isolate the indicator bacterial species E. coli and Enterococcus faecalis / E. faecium for antibiotic resistance pattern studies. Salmonella and the potentially human pathogenic verocytotoxic E. coli were not isolated, while Campylobacter jejuni jejuni was found in one roe deer sample only. Antibiotic resistance was found in 13 (7.3%) of the 179 E. coli isolates tested, eight of these being resistant against one type of antibiotic only. The proportion of resistant E. coli isolates was higher in wild reindeer (24%) than in the other cervids (2.2%). E. faecalis or E. faecium were isolated from 19 of the samples, none of these being reindeer. All the strains isolated were resistant against one (84%) or more (16%) antibiotics. A total of 14 E. faecalis-strains were resistant to virginiamycin only. The results indicate that the cervid species studied do not constitute an important infectious reservoir for either the human pathogens or the antibiotic resistant microorganisms included in the study. 相似文献
992.
Nonsteroidal anti-inflammatory drugs impair platelet aggregation and secretion in man, pigs, and rabbits and inhibit platelet thromboxane/prostaglandin synthesis. The present investigation studied the effects of phenylbutazone on platelet aggregation and bleeding times in the horse. Aggregation responses to adenosine diphosphate and collagen were markedly impaired 15 minutes and 2 hours after treatment, but 4 hours after treatment, platelet responses approximated those prior to treatment. The in vivo effect of phenylbutazone correlated with its plasma concentrations. Phenylbutazone, like aspirin, appeared to exert its effect by inhibiting thromboxane/prostaglandin synthesis, because thrombin-induced malondialdehyde formation was inhibited. However, unlike aspirin, free arachidonate-induced malondialdehyde synthesis was reduced but not eliminated, which suggested that phenylbutazone may have more than one site of action. Although collagen-induced platelet aggregation was impaired, a response was still present, and bleeding times were not altered by phenylbutazone treatment. To account for these findings, it is proposed that equine platelets can respond to collagen by thromboxane/prostaglandin independent pathways. The physiologic and pathophysiologic importance of these findings is discussed. 相似文献
993.
L K Nolan R E Wooley J Brown J B Payeur 《American journal of veterinary research》1991,52(9):1512-1517
Phenotypic characteristics of 12 paired, Salmonella serotypes isolated from healthy and ill chickens were compared. Variables compared included antibiotic resistance profiles, production of colicins and siderophores, mannose-sensitive hemagglutination of erythrocytes, resistance to serum complement, carbon source utilization, presence and transmissibility of R plasmids, and invasiveness in primary chicken kidney cell culture. Differences were found between pairs for utilization of carbon sources, mannose-sensitive hemagglutination of erythrocytes, and invasiveness in cell culture. 相似文献
994.
The Public Trust Doctrine (PTD) vests states with a fiduciary responsibility to manage wildlife for the benefit of current and future generations. States have varied approaches to applying the PTD for wildlife management ranging from a traditional focus on hunters, anglers, and trappers to a progressive approach of broad inclusion of all potential stakeholders in their decision-making processes. We argue that states need to gather and incorporate more and better human dimensions (HD) information to fulfill their PTD responsibilities. We describe some of the barriers to increased use of HD and the changes in agency culture, staffing, data gathering, and decision-making processes necessary to integrate HD effectively and comport with the PTD. We conclude that in addition to increasing fulfillment of PTD responsibilities, increased use of HD information will help maintain agency relevance, increase political support, and secure broader agency funding. 相似文献
995.
996.
D Schams F Graf J Meyer B Graule M Mauthner C Wollny 《Journal of animal science》1991,69(4):1583-1592
Twelve second-lactation Deutsches Fleckvieh cows, (FV, a dual-purpose breed) and 12 German Black and White (BW, a dairy breed) were used to evaluate similarities and differences in the response to a prolonged-release formulation of sometribove (N-methionyl bovine somatotropin, bST). Cows were milked twice daily and fed a basic diet (70% corn silage, 30% alfalfa hay) ad libitum plus 1 kg of concentrate. Additionally, concentrates and supplements were offered at the rate of 1 kg for each 22 kg of milk produced above 12 kg/d. Six cows of each breed were treated s.c. with 500 mg of sometribove at 14-d intervals from wk 9 to 29 postpartum (pp). Blood was collected by jugular vein puncture once weekly from wk 7 to 33 pp and daily for 14 d after the first and last (11th) injection of bST. Somatotropin (ST), insulin-like growth factor I (IGF-I), insulin, thyroid hormones, nonesterified fatty acids (NEFA), liver enzymes, and blood metabolites were evaluated. During bST treatment, plasma ST increased significantly on d 7 in both breeds and then decreased on d 14 after injection. Plasma IGF-I was positively correlated with ST. Plasma NEFA were high on d 7 and decreased on d 14. In response to bST, the average milk yield increased for FV and BW cows by 5.1 kg/d (29.7%) and 5.0 kg/d (28%), respectively, and 4% fat-corrected milk (FCM) increased by 5.0 kg/d (28%) and 5.3 kg/d (19.9%), respectively. Milk content of fat, protein, lactose, number of somatic cells, and body weight were similar across treatments.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
997.
Effects of activation methods on DNA synthesis and development of parthenogenetic porcine embryos 总被引:1,自引:0,他引:1
Ock SA Kwack DO Mohana Kumar B Han J Kim SW Rho GJ 《Reproduction in domestic animals》2011,46(6):1082-1089
This study investigated the timing of DNA synthesis and patterns of pronuclear (PN) formation during the first cell cycle, and its influence on developmental competence, velocity and proliferation index of porcine parthenote blastocysts produced by different activation treatments. Oocytes were activated as follows: electrical stimulation (EST), EST combined with 7.5 μg/ml cytochalasin B (EST + CCB), 10 μg/ml cycloheximide (EST + CHX) and 1.9 mm 6-dimethylaminopurine (EST + 6-DMAP) for 3 h. DNA synthesis and PN formation were evaluated using 1 mm 5'bromo-2'deoxy-uridne (BrdU) at 2 h intervals from 1 to 13 h or 5 to 13 h of post-activation (hpa), respectively. In EST, DNA synthesis started at 3 hpa, reached the peak at 11 hpa and decreased at 13 hpa. Treatment with 6-DMAP resulted in an early increase of DNA synthesis at 3 hpa, whereas CCB delayed DNA synthesis for 2 h. In EST and EST + 6-DMAP, most of the eggs showed 1PN, whereas, incidence of 2PN in EST + CCB was higher than 1PN. EST + CHX was observed with 1PN, 2PN and multiple PN. Blastocyst rate in EST + CCB and EST + 6-DMAP were significantly (p<0.05) higher than EST + CHX. But, the developmental velocity was not different among groups. Proliferation index of blastocysts, as indicated the number of blastomere at S-phase of the cell cycle was low in all groups. In conclusion, CCB, CHX and 6-DMAP used for producing porcine parthenogenetic embryos induced different onset of DNA synthesis and PN, but they did not affect the subsequent embryo development. 相似文献
998.
999.
1000.
Twenty feline vaccine-associated sarcomas were examined by transmission electron microscopy. Tumors contained pleomorphic spindle cells, histiocytoid cells, and giant cells. Most tumors contained myofibroblasts, which had morphologic features similar to those of fibroblasts. These cells were further distinguished by subplasmalemmal dense plaques and thin cytoplasmic actin myofilaments organized as elongated bundles concentrated at irregular intervals forming characteristic dense bodies. Intracellular crystalline particulate material was found in 5 of the 20 tumors. Energy dispersive X-ray spectroscopy was used to identify the crystalline material within one tumor as aluminum-based. One tumor from a feline leukemia virus-infected cat contained budding and immature retroviral particles. 相似文献