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排序方式: 共有594条查询结果,搜索用时 359 毫秒
581.
Ghanem Yasser M. El-Khodery Sabry A. Saad Ashraf A. Abdelkader Ahemd H. Heybe Ahemd Musse Yasin A. 《Tropical animal health and production》2009,41(8):1779-1786
The present study was delineated to investigate the prevalence and risk factors of camel brucellosis in Northern Somalia (Somaliland).
The study was carried out at three main districts of camel-rearing regions of Somaliland (Awdal, Waqoyi Galbed and Togdheer)
in the period from July to November, 2008. A total of 1246 camel blood sera were randomly collected from 42 sporadic small
scale camel herds. Two serological tests were used to screen all serum samples, Rose Bengal Plate Test (RBPT) and indirect
ELISA (I-ELISA). Multivariate logistic regression was constructed to study the risk factors associated with Brucella seropositive cases. The overall prevalence of camel brucellosis in districts under investigation was 3.9% by RBPT and 3.1%
by (I-ELISA). Multivariate logistic regression on animal level showed that locality (P < 0.05; OR: 6.254; CI, 1.186–32.976),
herd size (P < 0.001; OR: 5.493; CI, 2.956-10–207), rearing with other ruminants (P < 0.001; OR: 12.433; CI, 3.957–39.060),
and contact with other camels (P < 0.05; OR: 5.311; CI, 1.093–25.800) were the potential risk factors. However, herd size
(P < 0.05; OR: 5.425; CI, 1.181–24.932), and rearing with other ruminants (P < 0.05; OR: 20.466; CI, 1.456–28.638) were recorded
as risk factors on the herd level. The results of the present investigation indicate that the Brucella spp. exists within the camel herds in Somaliland. Further studies need to be done on Brucella infection in the other ruminants to determine which measure should be followed for control of brucellosis. 相似文献
582.
本研究从威海文登海域筛选获得一株产淀粉酶海洋菌W11,初步鉴定为弧菌,并探讨pH、温度、无机离子对淀粉酶活性和稳定性的影响及该酶底物浓度效应和Km值。结果表明:在pH7.5左右酶活性最高,pH在4.0~7.5范围内体现较强的稳定性。最适酶解温度为55℃,酶液在60℃以下有较好的热稳定性;Ba2+、Mn2+对淀粉酶有激活作用,而Cu2+、Mg2+、Zn2+则抑制淀粉酶活性,表观Km值为0.973mg/mL。海洋菌W11所产的中温淀粉酶保存温度范围较广、适应pH作用的范围广及稳定性较强,将有着广泛的应用潜力。 相似文献
583.
Katherine M. Phillips David M. Ruggio Mehdi Ashraf‐Khorassani Ronald R. Eitenmiller Sungeun Cho Linda E. Lemar Charles R. Perry Pamela R. Pehrsson Joanne M. Holden 《Cereal Chemistry》2010,87(1):42-49
Twelve popular ready‐to‐eat breakfast cereals fortified with folic acid were sampled in the United States in 2006, and the data have been incorporated into the USDA National Nutrient Database for Standard Reference. Cereals were collected from three statistically selected retail outlets in each of four primary census regions, and four composites of each product were prepared using random groupings of three locations each. Folic acid was determined using a validated LC‐MS method, with 13C‐folic acid as an internal standard, after trienzyme treatment and solid phase extraction. A cereal reference material (AACC VMA399) was analyzed as a control. Selected samples were also assayed using the standard microbiological method, with and without trienzyme extraction, to generate an estimate of endogenous folate. On average, as shown on the label, folate content was underestimated. In seven cereals, folate was within 5% of the declared value; in four cereals, it was 5–20% higher; and in two cereals, it was >20% greater, representing –75 to +69 μg/serving (mean 17) of the label value, equivalent to –19% to +17% of the 400 μg/daily value. The microbiologically determined folic acid was higher than LC‐MS by 10–67% (mean 40%). Therefore, use of label values might underestimate folate intake from some breakfast cereals. 相似文献
584.
光敏核不育水稻花粉发育的细胞学研究与PCD检测 总被引:5,自引:0,他引:5
应用石蜡切片技术和脱氧核糖核苷酸转移酶介导的缺口末端标记法(TUNEL),对光敏核不育水稻农垦58S和可育水稻农垦58N的花粉及花药壁发育过程进行了研究。结果表明:农垦58S雄性败育起始于花粉母细胞形成期,在发育过程中持续进行,终结于单核晚期,形成典败型花粉;TUNEL检测显示,农垦58S的绒毡层细胞在花粉母细胞时期启动细胞程序性死亡(PCD),但解体过程缓慢,一直持续到花粉发育后期,使单核花粉无法获得所需的营养物质而败育。 相似文献
585.
为解决牡丹果荚废弃物焚烧处理造成的环境污染及资源浪费问题,并进一步实现废弃物资源化利用,本文以生物质废弃物牡丹果荚为碳源,KHCO3为活化剂,且牡丹果荚与活化剂质量比为1∶3,经一步活化法800 ℃煅烧2 h制备出孔结构发达的牡丹果荚基多孔碳(PC-800-3)材料吸附水中四环素。通过调控活化温度、牡丹果荚/KHCO3质量比,得到的最佳产物 PC-800-3较直接热解的牡丹果荚基多孔碳(PC)比表面积增加60倍,298 K下对100 mg·L-1四环素溶液的最大吸附量为394.3 mg·g-1。通过比表面积孔径分析、扫描电子显微镜、傅里叶变换红外光谱、X射线衍射等对PC与PC-800-3进行表征,结果显示活化剂的加入促进了多孔碳中微孔与介孔的生成。选择PC-800-3进行后续吸附实验,吸附等温线和吸附动力学拟合结果表明吸附过程符合Langmuir等温模型和拟二级动力学模型,热力学实验证实吸附为自发吸热过程,以单分子层物理吸附为主,存在静电相互作用。所制备的PC-800-3材料在较宽pH范围内和离子干扰下的吸附性能良好。再生实验表明,PC-800-3具有良好的重复利用性能。因此,将牡丹果荚转化为多孔碳制备性能优异的吸附剂,在解决水环境中抗生素污染问题的同时拓宽了其应用范围,进一步实现了废弃生物质的资源化利用。 相似文献
586.
587.
Imad Khan Hafsa Zaneb Saima Masood Saima Ashraf Hafiz F. Rehman Sajid K. Tahir Habib U. Rehman Adnan Khan Raheela Taj Sadeeq U. Rahman Muqader Shah 《The Journal of Poultry Science》2022,59(3):272
The current study aimed to evaluate the efficacy of selenium nanoparticles (SeNPs), combined or loaded with chitosan (COS), in broiler chickens reared under standard management protocols. The parameters under investigation were production performance, organ development, components of the intestinal barrier, and ileal microbial count. Two hundred and forty day-old chicks were raised in five groups, with each group containing eight replicates (n=6/replicate). The control group received a basal diet whereas the other four groups received basal diets supplemented with SeNPs (0.5 mg/kg), COS (200 mg/kg), SeNPs+COS (0.5 mg/kg SeNPs + 200 mg/kg COS), and SeNPs-loaded COS (SeNPs-L-COS) (200 mg/kg) respectively. On day 35, two birds/replicate were sampled to collect the viscera under investigation. The results revealed that dietary inclusion of SeNPs-L-COS increased (p<0.05) the body weight gain and improved (p<0.05) feed conversion ratio. Similarly, SeNPs-L-COS supplementation increased (p<0.05) the small intestinal villus surface area as well as the count of acidic goblet cells and intraepithelial lymphocytes when compared with the control group. Whereas the total goblet cell count was higher (p<0.05) in the small intestines of both the SeNPs+COS and SeNPs-L-COS groups. Microbial analysis of ileal contents also revealed an increase (p<0.05) in Lactobacilli species count with a concurrent decrease (p<0.05) in Escherichia coli count in the SeNPs-L-COS group when compared with the COS and control groups. Based on the results of the current trial, we can conclude that supplementation with SeNPs-L-COS is a superior combination for promoting the gut health and performance of broilers. 相似文献
588.
589.
590.
检索中国银行业1985~2012年三类专利(发明专利、实用新型专利和外观设计专利)的申请总量、申请年度趋势、主要银行申请数量、专利发明人数、专利涉及的主要IPC分类号等信息,考量中国银行业的专利发展整体状况,特别是最具技术创新代表性的发明专利,结果显示:外资银行比内资银行更注重发明专利,外资银行发明专利的发展比内资银行起步要早,但发展较平缓;内资银行发明专利发展较快,申请数量高于外资银行。鉴此,中国银行业需加大对发明专利的研发投入,培养科技创新人才,提升专利发明质量。 相似文献