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61.
Masaki Jinkawa Hirokazu Yamaguchi Kuniaki Furukawa Toshiaki Satake Hiroshi Kobayashi 《Journal of Forest Research》2006,11(2):77-88
For monorail systems, it is very important to ensure the safety of the ground structure. We developed a tram car for slopes
that utilizes the mechanism of the monorail and analyzed the stress of the ground structure that had double rails. First,
we divided the ground structure into seven components, and formulated a procedure for calculating the stress of the ground
structure. As a result, the stress that acts upon the main rail and the main rail connecting part was near the allowable stress,
and each calculated value was within the allowable stress. We measured the stress by running the vehicle, and compared the
measured values with the calculated values. As a result, some measured values were larger than the calculated values. However,
the ratio of measured value to allowable stress was 90% or less. Finally, we calculated the static loading coefficient for
each part, results of which were as follows: main and subrail: 1.1; rail connection part and prop foundation part: 1.0; rail
installation part: 1.3. In the recalculations, it became clear that the recalculated values exceeded the allowable stresses.
These results suggest that we need to review the ground structure materials. 相似文献
62.
Treatment by Sivanesan and Gibson (1976), that combined all species of Entomosporium into one species E. mespili (DC. ex Duby) Sacc, was supported through the investigation of many exsiccati and fresh specimens.Morphologically, all specimens and isolates of Entomosporium, which had been differentiated into 4 species, could not be divided into clearly separated groups.The fungus has been recorded on 50 host species and the disease is widespread throughout the world. 相似文献
63.
Y. Yoshiura Y.C. Sohn A. Munakata M. Kobayashi K. Aida 《Fish physiology and biochemistry》1999,21(3):201-210
A cDNA encoding the subunit of thyrotropin (TSH) was isolated from a goldfish (Carassius auratus) pituitary gland cDNA library. By comparing the sequence with other teleost TSHs, a signal peptide of 19 amino acids and a mature hormone of 131 amino acids were predicted for goldfish TSH subunits. The resulting putative mature hormone of 131 amino acids had well-conserved cysteine positions and a putative N-linked glycosylation site; homology was 51–67% with TSHs from other teleosts, 38–43% with tetrapod TSHs, but only 27 and 29% with goldfish GTH-I and -II, respectively. We also examined the effects of thyroid hormones (TH) and thiourea (TU, an inhibitor of TH production) treatments on TSH and GTH subunit gene expressions in the goldfish pituitary gland. After thyroxine (T4) treatment, circulating T4 concentration increased and TSH mRNA level decreased. Supressing the amount of circulating T4 and triiodothyronine (T3) by TU treatment increased the TSH mRNA level. Moreover, T4 replacement therapy (simultaneous treatment of both TU and T4) caused a high level of circulating T4 and a low level of circulating T3, and a decrease in the TSH mRNA level. Thus, changing levels of circulating TH exert a negative feedback on the level of TSH subunit mRNA in goldfish in vivo. On the other hand, GTH subunit mRNA levels were not affected by changes in the levels of circulating TH. 相似文献
64.
S. Kobayashi A. HidanoT. Tsutsui T. YamamotoY. Hayama T. NishidaN. Muroga M. KonishiK. Kameyama K. Murakami 《Research in veterinary science》2014
This cross-sectional study evaluated risk factors associated with farm-level bovine leukemia virus (BLV) seropositivity in 563 dairy and 490 beef farms throughout Japan. Twenty randomly selected cattle on each farm were serologically tested, and farm epidemiologiocal information was obtained through face-to-face interviews. Due to the large number of zero-prevalence dairy and beef farms, data analysis was performed using a zero-inflated negative binomial model, which revealed that the common risk factors associated with higher within-farm seroprevalence were past detection of clinical leukemia and presence of blood-sucking insects. Loose housing on dairy farms and direct contact between calves and adult cattle on beef farms were also identified as risk factors. With regard to farm-level presence of BLV, the presence of purchased cattle was found to be a risk factor in both sectors. Sending heifers to a common ranch was identified as an additional risk factor for dairy farms. 相似文献
65.
Minoru Okabe Motoi Yasumura Kenji Kobayashi Kazuhiko Fujita 《Journal of Wood Science》2014,60(1):49-58
Cross-laminated timber (CLT) panels consist of several layers of lumber stacked crosswise and glued together on their faces. Prototype sugi CLT floor panels were manufactured and bending tests were carried out under the different parameters of lumber modulus of elasticity (MOE), number of layers, thickness of lumber and thickness of CLT panels. On the basis of above tests, bending stiffness and moment carrying capacity were predicted by Monte Carlo method. MOE of lumber was measured by using grading machine and tensile strength of lumber was assumed to be 60 % of bending strength based on the obtained bending test. Bending stiffness EI of CLT panels could be estimated by adopting composite theory and equivalent section area. Experimental moment carrying capacity showed 12 % higher value than the calculated moment carrying capacity by average lumber failure method, and also showed 45 % higher value than the calculated moment carrying capacity by minimum lumber failure method due to the reinforcement of the outer layer by the neighboring cross layer. 相似文献
66.
Masaru Matsuda Yoshitaka Nagahama Tohru Kobayashi Chika Matsuda Satoshi Hamaguchi Mitsuru Sakaizumi 《Fish physiology and biochemistry》2003,28(1-4):135-139
Although the sex-determining gene Sry has been identified in mammals, no comparable genes have been found in non-mammalian vertebrates. To clone positionally the sex-determining region of the medaka, Oryzias latipes, we generated a Y congenic strain to highlight the genetic differences between the X and Y chromosomes from inbred strains of medaka. We used recombinant breakpoint analysis and deletion analysis of the Y chromosome of a congenic XY female to restrict the sex-determining region to 250-kb stretch of the Y chromosome. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only one gene was Y specific. The full-length cDNA sequence of this gene encodes a putative protein of 267 amino acids, including the highly conserved DM domain. We thus named it DMY. To establish a role for DMY during sexual differentiation, we screened wild medaka populations for naturally occurring DMY mutants. Two XY females with distinct mutations in DMY were found in separate populations. The first heritable mutant – a single insertion in exon 3 and the subsequent truncation of DMY – resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. Furthermore, during normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for normal testicular development and is a prime candidate for the medaka sex-determining gene. 相似文献
67.
Takumi Shinkai Takaaki Ueki Satoshi Koike Yasuo Kobayashi 《Animal Science Journal》2014,85(3):254-261
To determine the relationship between Fibrobacter succinogenes and other rumen bacteria, the bacterial community structure on fiber was analyzed by using two different materials. These were ruminally incubated orchard grass hay stems without and with preincubation with F. succinogenes (natural and artificial consortia, respectively). The natural consortium mainly consisted of Firmicutes (56.6%) and Bacteroidetes (33.1%), while the artificial consortium showed a significantly higher proportion of Firmicutes (85.5%) and a lower proportion of Bacteroidetes (4.6%). At species or genus level, Butyrivibrio fibrisolvens, the U2 group, Ruminococcus albus and Lachnospiraceae incertae sedis made up a higher proportion in the artificial consortium. The most dominant bacterial group was the Butyrivibrio‐Pseudobutyrivibrio‐Lachnospiraceae incertae sedis group, which accounted for 19.7% in the natural and 29.5% in the artificial consortium. Within the genus Butyrivibrio, the phylogenetic groups SA and VA2 and phylogeny‐undefined Butyribivrio, but not VA1, were detected at high frequency in the artificial consortium. These results suggest that ecological and possibly functional relationships exist in the rumen among F. succinogenes, a subset of B. fibrisolvens, the U2 group, R. albus and Lachnospiraceae incertae sedis. 相似文献
68.
Transgenic cell lines which stably express progestogen receptors (PRs) and the PR-responsive reporter genes were developed. They are a good system for rapid, sensitive and reproducible screening of various ligands. 相似文献
69.
70.
Goldfish,Carassius auratus, spawn several times within a spawning season. A gonadotropin (GtH) surge occurs at the time of ovulation in this cyprinid
species. This ovulatory GtH surge mediates the processes of final oocyte maturation and ovulation, and occurs at the end of
each spawning cycle. Within a cycle, there is a shift in the predominant plasma steroid from estradiol to testosterone, and
finally 17α, 20β-dihydroxy-4-pregnen-3-one at the time of the ovulatory GtH surge. High levels of testosterone were always
observed before ovulation. When ovariectomized or sexually regressed female goldfish were implanted with testosterone, they
exhibited a GtH surge which was similar to those normally observed at ovulation. These results strongly suggest that elevated
plasma testosterone is an important physiological requirement for the occurrence of the GtH surge. 相似文献