Natural cortisol production is not linked to the sexual fate of European sea bass

Fish Physiology and Biochemistry - In this study, we aimed to investigate the relationship between cortisol and the determination of sexual fate in the commercially important European sea bass...     

Concentration and mean degree of polymerization of Rubus ellagitannins evaluated by optimized acid methanolysis

Ellagitannins are a major class of phenolics largely responsible for the astringent and antioxidant properties of raspberries and blackberries. The Rubus ellagitannins constitute a complex mixture of monomeric and oligomeric tannins. Rubus oligomeric ellagitannins contain, beside the well-known ellagic acid and gallic acid moieties, the sanguisorboyl linking ester group. When exposed to acids or bases, ester bonds are hydrolyzed and the hexahydroxydiphenic acid spontaneously cyclizes into ellagic acid. This study describes a new, rapid procedure for the acid hydrolysis of Rubus ellagitannins in methanol, which results in maximal yield and enables the quantification of all the major reaction products. Additionally, the method provides the rationale for estimating the mean degree of polymerization of Rubus ellagitannins.