Comparison Between Elisa and Dye Test for Detection of Naturally Acquired Toxoplasma Gondii Antibodies in the Goat

Toxoplasma gondii IgG antibodies were measured in 212 goat sera, comparing the Sabin-Feldman dye-test and a three-layer sandwich enzyme-linked immunosorbent assay (ELISA). With 98 % concordance obtained between these 2 tests, the results are at the same paragon as for human sera. Accordingly, the ELISA sandwich procedure appears to be suitable for large-scale analysis of goat sera. The discordant 2 % were ELISA positive and dye-test negative. One possible explanation of the divergent titres is given using an immunized goat model.Key words: Toxoplasma gondii, goat, antibodies, dye-test, ELISA     

Bovine tricuspid endocarditis as a cause of increased serum concentration of cardiac troponins

A Holstein cow presented for weight loss and anorexia had tachycardia, heart murmur, and a chronic inflammatory process. Serum cardiac troponin I was increased at 3.52 ng/mL. Transthoracic echocardiography revealed a thickened tricuspid valve and comet-tail artifacts compatible with gas in the affected area. This report suggests that serum cardiac troponin I may be increased in bacterial endocarditis in cattle.     

Sow removal in Swedish commercial herds

The objective of this study was to describe the sow removal pattern in Swedish commercial piglet producing herds, with group-housing of non-lactating sows. The data collection, based on a PC herd monitoring program used in 21 piglet producing herds in the south-central part of Sweden, was prospective, started in 2002 and continued for 3 years. During this period, information on 14 234 removed sows was recorded. For all parameters studied a large variation between herds was found. The average annual removal rate was 49.5% (range 33.6–66.3%). Among the removed sows, 85.2% were sent to slaughter, 10.5% were euthanised and 4.3% were found dead. The most common removal reasons were ‘reproductive disorders’ (26.9%), ‘old age’ (18.7%) and ‘udder problems’ (18.1%), followed by ‘low productivity’ (9.5%), ‘lameness and/or foot lesions’ (8.6%) and ‘traumatic injuries’ (7.1%). The proportion of sows sent to slaughter was higher than the proportion euthanised for all categories of removal except ‘traumatic injuries’ (in which 81.6% of the sows were euthanised). The average parity number at removal for all sows was 4.4. Lowest average parity numbers were found for sows removed due to ‘traumatic injuries’ (2.6). Among removed sows, the proportion removed per parity decreased up to parity 6. Sows removed due to ‘old age’ had the highest piglet production, while sows removed due to reproductive disorders had the highest number of non-productive days (NPD) per parity and the longest interval from last farrowing to removal. With increasing removal parity number, the number of piglets produced increased and NPD decreased. In lower parities (1–3) most sows were removed due to ‘reproductive disorders’, but this proportion fell as parity number rose. Removals due to ‘udder problems’ were most frequent in medium parity numbers (4–6). Most sows removed after parity 7 were removed due to old age. The proportion of the sows found dead was highest just after farrowing; otherwise only a low percentage of sows were removed during the lactation period. The proportion of euthanised sows was high in the week after farrowing and during the 4-week period following weaning. The proportion of sows sent to slaughter increased considerably 4 weeks after farrowing (i.e. after weaning) and stayed high during the subsequent 3 weeks. The study revealed a considerable amount of unplanned removal of young sows. Not only the sows sent to slaughter, but also those euthanised and found dead, must be included in studies on sow removal.     

Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland

Background

Sex steroid hormone receptors have been identified in the adrenal gland of rat, sheep and rhesus monkey, indicating a direct effect of sex steroids on adrenal gland function.

Methods

In the present study, immunohistochemistry using two different mouse monoclonal antibodies was employed to determine the presence of oestrogen receptor alpha (ERalpha) and progesterone receptor (PR) in the mare adrenal gland. Adrenal glands from intact (n = 5) and ovariectomised (OVX) (n = 5) mares, as well as uterine tissue (n = 9), were collected after euthanasia. Three of the OVX mares were treated with a single intramuscular injection of oestradiol benzoate (2.5 mg) 18 – 22 hours prior to euthanasia and tissue collection (OVX+Oe). Uterine tissue was used as a positive control and showed positive staining for both ERalpha and PR.

Results

ERalpha staining was detected in the adrenal zona glomerulosa, fasciculata and reticularis of all mare groups. Ovariectomy increased cortical ERalpha staining intensity. In OVX mares and one intact mare, positive ERalpha staining was also detected in adrenal medullary cells. PR staining of weak intensity was present in a low proportion of cells in the zona fasciculata and reticularis of all mare groups. Weak PR staining was also found in a high proportion of adrenal medullary cells. In contrast to staining in the adrenal cortex, which was always located within the cell nuclei, medullary staining for both ERalpha and PR was observed only in the cell cytoplasm.

Conclusion

The present results show the presence of ERalpha in the adrenal cortex, indicating oestradiol may have a direct effect on mare adrenal function. However, further studies are needed to confirm the presence of PR as staining in the present study was only weak and/or minor. Also, any possible effect of oestradiol treatment on the levels of steroid receptors cannot be determined by the present study, as treatment time was of a too short duration.     

Correspondence regarding 'Clouded leopards,the secretive top-carnivore of South-East Asian rainforests: their distribution,status and conservation needs in Sabah,Malaysia'

Correspondence regarding Wilting A, Fischer F, Bakar SA, Linsenmair KE: Clouded leopards, the secretive top-carnivore of South-East Asian rainforests: their distribution, status and conservation needs in Sabah, Malaysia. BMC Ecol 2006, 6:16.     

Antidiabetic activity of red wine polyphenolic extract, ethanol, or both in streptozotocin-treated rats

A polyphenol extract from a Corbières (France) red wine (P, 200 mg/kg), ethanol (E, 1 mL/kg), or a combination of both (PE) was administered by daily gavage for 6 weeks to healthy control or streptozotocin (60 mg/kg i.v.)-induced diabetic rats (180-200 g). Treatment groups included C or D (untreated control or diabetic) and CP, CE, or CPE (treated control) or DP, DE, or DPE (treated diabetic). P treatment induced a reduction in body growth, food intake, and glycemia in both CP and DP groups. In DP, hyperglycemia was reduced when measured 1 h after daily treatment but not at sacrifice (no treatment on that day). The hyperglycemic response to the oral glucose tolerance test (OGTT) and plasma insulin at sacrifice were impaired similarly in DP and D groups. In contrast, in DE or DPE, body growth was partially restored while hyperglycemia was reduced both during treatment and at sacrifice. In addition, hyperglycemia response to OGTT was reduced and plasma insulin was higher in DE or DPE than in D animals, indicating a long-term correction of diabetes in ethanol-treated animals. Morphometric studies showed that ethanol partially reversed the enlarging effect of diabetes on the mesenteric arterial system while the polyphenolic treatment enhanced it in the absence of ethanol. In summary, our study shows that (i). a polyphenol extract from red wine ("used at a pharmacological" dose) reduces glycemia and decreases food intake and body growth in diabetic and nondiabetic animals and (ii). ethanol ("nutritional" dose) administered alone or in combination with polyphenols is able to correct the diabetic state. Some of the effects of polyphenols were masked by the effects of ethanol, notably in diabetic animals. Further studies will determine the effect of "nutritional" doses of polyphenols as well as their mechanism of action.     

When agroforests drive back natural forests: A socio-economic analysis of a rice-agroforest system in Sumatra

In the Krui arae, (South Sumatra, Indonesia), the damar agroforests (Shorea javanica, Dipterocarpaceae) are but one element of the farming systems which include several other cropping systems such as irrigated or rainfed rice cultivation, and coffee plantation.Rainfed rice is cultivated in forest clearings, in order to meet rice requirements which cannot be met with irrigated rice crop only. In the forest clearings, coffee stands are established in rice crop, and later, Damar are planted among coffee plants. As a result of this cropping system, the damar agroforest spreads while the forest dwindles.In one village, no more forest land is available for peasants' clearings. The resulting high land pressure causes cropping systems and farming systems to change or to be exported to neighbouring areas where forest land is still available.

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Résumé Les agroforêts à damar (Shorea javanica) ne sont qu'un élément du système d'exploitation agricole de la région de Krui, Sud Sumatra, Indonésie. Ceux-çi associent plusieurs systèmes de culture tels que la riziculture irriguée, la culture du riz pluvial et la plantation de cafeiérs.L'insuffisance des surfaces en rizières irriguées est compensée par la culture du riz pluvial, pratiquée en ladang, parcelle de forêt défrichée et brûlée. Sur les ladangs se succèdent les cultures de riz, de café puis de damar, transformant ainsi la forêt naturelle en agroforêt à damar. On assiste à l'extension continue des agroforêts au détriment de la forêt naturelle.Dans un des villages étudiés, la réserve de terres forestières communales est épuisée, déjà transformée en agroforêts. La forte pression fonçière qui en résulte induit des transformations dans les systèmes de production agricole ou incite des agriculteurs à migrer dans des régions voisines encore riches en terres forestières. Dans ces régions, les migrants reproduisent un système agroforestier très proche de celui de Krui, dans la mesure où les conditions écologiques le permettent.

     

Regulation of replication fork progression through histone supply and demand

DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone chaperone Asf1 and MCM2-7, the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of Asf1 by RNA interference impedes DNA unwinding at replication sites, and similar defects arise from overproduction of new histone H3-H4 that compromises Asf1 function. These data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.