Aluminium uptake by roots of rye seedlings of differing tolerance to aluminium toxicity

Summary The aluminium accumulation pattern was studied in roots and root tips of rye seedlings from two inbred lines, differing in Al-tolerance and their F₁ hybrid.This investigations were undertaken in order to find whether the mechanism preventing root tips from to high Al-accumulation and damage to apical meristem observed in Al-tolerant isoline of wheat is also operating in rye. Al-uptake into the root tissue was studied in nutrient solution experiments during 24 h of incubation in solution containing aluminium. After Al-incubation roots were harvested, separated into root tips and basal parts, and aluminium content in root tissue was determined after wet digestion using catechol violet colorimetric method. It was found, that at low external Al-concentration in rye roots the mechanism preventing root tips from Al-accumulation and Al-damage operates, similarly as in Al-tolerant wheat isoline. But contrary to wheat in rye seedlings functioning of this mechanism was observed in both Al-tolerant and Al-sensitive lines. It is concluded that observed mechanism of the inhibition of Al-accumulation in apical part of roots plays much more important role in Al-tolerance mechanism in wheat than in rye.Abbreviations EDTA ethylenediaminetetraacetic acid - NIL near-isogenic line     

Why asparagine needs carbohydrates to generate acrylamide

Structural considerations dictate that asparagine alone may be converted thermally into acrylamide through decarboxylation and deamination reactions. However, the main product of the thermal decomposition of asparagine was maleimide, mainly due to the fast intramolecular cyclization reaction that prevents the formation of acrylamide. On the other hand, asparagine, in the presence of reducing sugars, was able to generate acrylamide in addition to maleimide. Model reactions were performed using FTIR analysis, and labeling studies were carried out using pyrolysis-GC/MS as an integrated reaction, separation, and identification system to investigate the role of reducing sugars. The data have indicated that a decarboxylated Amadori product of asparagine with reducing sugars is the key precursor of acrylamide. Furthermore, the decarboxylated Amadori product can be formed under mild conditions through the intramolecular cyclization of the initial Schiff base and formation of oxazolidin-5-one. The low-energy decarboxylation of this intermediate makes it possible to bypass the cyclization reaction, which is in competition with thermally induced decarboxylation, and hence promote the formation of acrylamide in carbohydrate/asparagine mixtures. Although the decarboxylated Amadori compound can be formed under mild conditions, it requires elevated temperatures to cleave the carbon-nitrogen covalent bond and produce acrylamide.     

Field Data of Hydrogen Peroxide Concentrations in Urban and Mountain Air in Southwestern Poland

The concentrations of ambient gas-phase hydrogen peroxide were measured during the summer of 1998, 1999 and 2000. The experiments were performed in the city of Wroclaw and in the vicinity of Mount Szrenica, 1362 m a.s.l., Poland. Analysis was carried out by the chemiluminescence method. Typical mean ranges of 30 min H₂O₂ concentrations measured were 1.4–6.0 μg m^-3 at Mount Szrenica, whereas in the urban atmosphere H₂O₂ concentrations were in the range of 2.7–11.7 μg m^-3. In the case of the urban atmosphere, H₂O₂ concentrations were well correlated only with solar radiation and temperature. In the mountain air, H₂O₂ concentrations increased along with the increase of temperature, O₃, CO and the decrease of humidity. The diurnal variation was not only caused by photochemicalprocesses.     

Distribution of Protura along an urban gradient in Vienna

In the first study of the Protura of a big city, six representative habitat types were sampled in Vienna, Austria. Twenty-five sites between the green belt and the city centre yielded 42 known species. Abundance, species number and diversity were highest in colline deciduous forests, with a maximum of 23 syntopic species in a Quercus pubescens stand. Acerella muscorum, all of the five Acerentomon spp. and 13 further species occurred exclusively in colline deciduous forests. The mediterranean Berberentulus berberus and six other species were recorded exclusively in parks or artificial habitats, suggesting anthropogenic transport. No Protura were found in garden and arable soils. Species density in the municipal area of Vienna (415 km²) is a function of the city's biogeographic situation on the border between the Alps and the Pannonian lowland as well as its diversity of seminatural habitats, and unintentional introduction.     

Differences in C and N release from Alliaria petiolata leaves and stems: consequences for nutrient cycling in forest ecosystems

European Journal of Forest Research - Garlic mustard (Alliaria petiolata (Bieb.) Cav. et Grande) is a widespread forest edge plant species in the European temperate zone and also one of the most...     

Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol

The aim of the study was to evaluate the selected lymphocyte subpopulations TCD4, TCD8, BCD21, BCD25, CD18, CD11b, and MHC II in blood and uterine flush of cows with endometritis, before and after intrauterine (i.u.) administration of cefapirin and methisoprinol. The research was carried out on 28 cows with clinical endometritis. Animals were divided into four groups, each composed of seven cows, depending on the i.u. preparation used: Group A, cefapirin; Group B, methisoprinol; Group C, cefapirin and methisoprinol simultaneously; and a control group—without medication. The study was performed using flow cytometry method. Summarizing the results of the research, i.u. infusion of cefapirin caused a weakening of the effector phase of the local uterine immune response; however, it enhanced leukocyte chemotaxis and antigen presentation. After i.u. administration of methisoprinol, the stimulation of specific uterine immunity mechanisms was mainly observed. The use of both mentioned preparations showed the strengthening of specific uterine immunological mechanisms presumably caused by methisoprinol, despite the inhibitory effect of the antibiotic. Intrauterine use of immunostimulatory substances can improve the effectiveness of the endometritis treatment in cows by improving specific local mechanisms of uterine immunity. As a consequence, it may enhance the effector function of immune competent cells and finally eliminate inflammation.     

Inheritance of the efficiency of nitrogen uptake and utilization in winter wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) under diverse nutrition levels

The effects of contrasting soil nitrogen (N) levels on the inheritance of the efficiency of N uptake and N utilization in grain mass formation were examined in winter wheat. Parents of various origins and their diallel F₂-hybrids were evaluated in field and pot experiments under varying levels of soil N. The range of additive variance in the components of N efficiency was narrow, especially under N shortage. The soil N-treatments imposed had a substantial influence on gene actions responsible for the efficiency components and modes of inheritance. Genotype × nutrition interactions were common. Under high N-fertilization, the efficiency components were inherited in a manner favourable for wheat selection (preponderance of additive effects). However, the enhanced contribution of non-additive gene effects and increased dominance under N-limited conditions could impede wheat selection to improve the N efficiency and adaptation to less luxurious fertilization regimes. Selection methods that eliminate masking non-additive influences and take advantage of the additive variance should be employed to improve these traits.     

Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout (Oncorhynchus mykiss) semen

During the reproductive season, rainbow trout spermatozoa are stored in the sperm ducts for several months. There is no sperm production at this time since spermatogenesis is completed before spawning. To leam more about characteristics of semen during such a long storage, we analyzed changes in protein concentrations, anti-proteinase activity in seminal plasma and sperm aspartate aminotransferase activity during an extended reproductive period during which fish were fed diets supplemented with various ascorbic acid concentrations. Seminal plasma protein concentration and anti-proteinase activity declined toward the end of the reproductive season. These phenomena may be related to oncoming proteolytic events leading to degradation of the sperm. Protein concentrations and anti-proteinase activities were strongly correlated within groups of different ascorbic acid supplementations and several sampling dates (r=0.6–0.9 in most cases, p<0.05). Ascorbic acid deficiency resulted in a decrease in both parameter levels as compared to levels in groups with vitamin C supplement (p<0.08). Deficiency also resulted in lower stimulation of aspartate aminotransferase by an exogenous pyridoxal 5-phosphate in comparison to fish fed vitamin C-supplemented diets (p<0.05). These results support earlier studies suggesting a protective role of ascorbic acid toward maintaining sperm quality.     

Genetic and historical studies on the origin of Norway spruce in Białowieża Primeval Forest in Poland

Białowieża Primeval Forest lies within the boreal part of the Norway spruce range, which, according to pollen data, was formed by a refugium located in the Russian Upland. However, recent genetic studies have indicated that spruce from the Eastern Carpathian refugium also occurs in Białowieża Forest. In order to investigate whether the Norway spruce from the Eastern Carpathian refugium migrated naturally into the boreal part of the Norway spruce range, we assessed the refugial origin of 234 of the oldest Norway spruce individuals from Białowieża Forest using mitochondrial marker mt15-D02. Out of the trees that we studied, 64.8% carried mitotype 1, which is specific to the Carpathian refugium, while 35.2% had mitotype 3, specific to the Russian refugium. Our genetic analysis, combined with historical data on forest management in this part of Europe, argues for a natural contribution of spruce from the Eastern Carpathian refugium during postglacial recolonization of Białowieża Primeval Forest.