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81.
Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran 总被引:1,自引:0,他引:1
Akbar Keshavarz Ali Haghighi Amid Athari Bahram Kazemi Alireza Abadi Ehsan Nazemalhosseini Mojarad 《Veterinary parasitology》2009,160(3-4):316-318
The aim of this study was to determine the prevalence, variability with host age, and the genotypes of species of Cryptosporidium in cattle from 15 dairy farms in Qazvin province, Iran. Fecal samples, collected from 272 cattle during May 2006 to December 2007, were characterized microscopically. Oocysts from 51 positive samples were analyzed using PCR assay of 18S SSU rRNA, restriction fragment length polymorphism (RFLP) and sequencing. We identified 72.6% of the positive samples as Cryptosporidium parvum, 17.7% as Cryptosporidium andersoni, 7.8% as Cryptosporidium bovis and 1.9% as a novel genotype of C. parvum possessing a single mutation on MboII restriction. An infection rate of 19.5% of C. parvum among 174 pre-weaned calves was significantly higher than the 3.1% among 98 post-weaned calves (P < 0.0006). This is the first report of C. bovis and the new subgenotype of C. parvum in Iranian cattle. 相似文献
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Hamed Karami Shabankareh Seyed Behnam Seyedhashemi Mehran Torki Hamidreza Kelidari Alireza Abdolmohammadi 《Tropical animal health and production》2012,44(8):1865-1871
The objective of this study was to evaluate if treatment of equine chorionic gonadotrophin (eCG)-superovulated Sanjabi ewes with repeated administration of human chorionic gonadotropin (hCG) would increase the number of normal corpus luteum (CL) and serum progesterone concentrations and decrease the number of persistent follicles. The superovulated ewes were divided into four groups on day 0 (day of sponge removal); the ewes were treated by an intramuscular administration of 500?IU hCG on day 0 (Group I: n?=?10), on days 0 and 1 (Group II: n?=?10), or on days 0, 1, and 2 (Group III: n?=?10) and no treatment for control group (n?=?10). Blood samples were collected on days 0, 1, 2, 5, and 8 (day of slaughter), and serum progesterone concentrations were determined. According to progesterone concentrations, 50 (4/8) and 0?% of the ewes underwent premature luteal regression in the control group and the hCG groups, respectively. There were more CLs in Group III than in Group II and the control group. Ewes treated with hCG had a greater number of normal-looking CL. CL diameter was significantly greater in Group II and Group III than other groups. Total CL weight was significantly (P?<?0.05) higher in Group III than in Group I and the control group. Number of persistent follicle and persistent follicle diameter were lower in control group compared to the other groups. Eight days after sponge removal, serum progesterone concentration was significantly higher in Group III than in Group I and the control group. The present results indicate that repeated administration of hCG supported CL formation, increased serum progesterone concentration, and prevented premature luteal regression in eCG-superovulated Sanjabi ewes. 相似文献
84.
To investigate the effects of agronomic practices and farmer demographics on levels of weed infestation in chickpea fields, a survey was conducted during 2015 in Sanjabi district, Kermanshah, Iran. Two sets of data were collected: (a) weed sampling from the selected chickpea fields, and (b) questionnaire recording of farmer demographics and agronomic management. The farmers were asked about their residence status (resident or migrant), occupation (farming only, farming plus a second job), experience and education levels, as well as seedbed preparation, sowing date and method, source of seed supply, crop cultivar, sowing seed rate, weed control operation and crop rotation history. The results showed that weed species composition was similar across the studied fields. The use of a row crop planter, a sowing rate of 45 kg ha‐1, and weed control resulted in 30.2, 23.03 and 34.2% reductions in weed infestation compared with hand sowing, a sowing rate of 30 kg ha‐1 and lack of weed control, respectively. Weed density decreased with increasing farmer experience and decreased by 33.0% and 23.5% in chickpea fields of resident farmers and owners whose sole occupation was agriculture. We found that 89.5% of the most experienced farmers adopted weed control operations and most resident landowners (77.4%) opted for crop planter (77.4%) and weed control (90.3%) compared to migrant farmers (50 and 68.5%, respectively). More landowners who were exclusively farmers performed weed control (90.6%) than owners who had a second job (67.9%). 相似文献
85.
Nakhaee A Taheri T Taghikhani M Mohebali M Salmanian AH Fasel N Rafati S 《Veterinary parasitology》2004,119(2-3):107-123
Canids are natural reservoirs of Leishmania infantum and have been promoted as experimental hosts to decipher the pathogenesis of human visceral leishmaniasis (VL). In this study, the presence of IgG antibodies as well as the presence of mononuclear leukocytes reactive to different cysteine proteinases (CPs) were examined in 13 L. infantum-infected dogs (six with symptoms, seven asymptomatic). Cysteine proteinases which belong to papain-like enzymes known as clan CA are the most studied CPs of parasite protozoa. These molecules are expressed by the intracellular stages of the parasite and could be immunogenic. We studied Type II CP (CPA) and Type I CP (CPB) with its long C-terminal extension (CTE) which could be highly immunogenic. We showed that the level of antibodies reactive to rCPA is low in both symptomatic and asymptomatic dogs. In contrast, when CPB and CTE were used as antigens, the level of total IgG (with IgG2 superior to IgG1) reached higher values in asymptomatic dogs than in dogs with VL. While the peripheral blood mononuclear cell (PBMC) reactivity was significant when cultured in the presence of freezed/thawed (F/T) lysate, it remained low in presence of CP although always higher for PBMC recovered from asymptomatic dogs. We showed the importance of CPB and CTE in particular as a target of immune response and their potential use for serodiagnosis in asymptomatic dogs. 相似文献
86.
Bita Naseri Alireza Marefat 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(2):179-195
The relationships between a number of agronomic practices, Fusarium root rot (FRR) measurements and yield variables were characterized
at different growth stages in 122 commercial bean fields in Zanjan, Iran. Mean FRR incidence, severity and index differed
among growth stages and years. A lower FRR index at growth stage R9 and higher yields were detected in Red beans compared
to Pinto and White beans. FRR severity affected the number of pods and seeds per plant. Despite the lack of an impact of initial
drought on FRR and yield factors, FRR levels were higher following frequent irrigations at 2–3 days intervals than at longer
intervals at R6-7. The highest FRR index at R6-7 (51.9%) and incidence at R9 (69.1%) were associated with the densest category
of plant populations. Yield components differed significantly between the levels of plant density at R9. FRR levels at V3
were lower for June plantings than for May-second-week plantings. Lower FRR ratings and higher yields were detected at a seeding
depth of 0–5 cm in comparison to 10–20 cm. At R9, FRR levels on beans following maize were lower than those following barley,
bean, tomato or wheat. At V3, FRR severity was greater in fields that received 50–500 kg/ha of urea compared to non-fertilized
fields. Greater mean FRR index at R6-7 and lower yield levels were associated with the greatest weed density compared to weed-free
fields. This new information benefits the systematic understanding of interactions between bean yield, FRR and various agronomic
variables at large-scale. 相似文献
87.
The present study on the sediment production in natural forests was carried out on an 13,284 ha area,ocated in Guilan Province, in the north of Iran. This area is under intensive management. We first analyzed the physi- sal factors of the area. Then, the amount of road construction, afforestation and harvested wood volume, as well as the harvesting method in the region were determined for the years between 1993 and 2010. The results show that the aver- age annual amount of sediment production in the entire region, over this 18-year period, has increased annually at a rate of 3.54 t.km-2 (R2 = 0.56) at a confidence level of 95%. Operations management and management methods used in :his forest area has affected the amount of sediment production. Clear cutting and extensive harvesting methods in the region caused an increase in the amount of sediment production, as indicated by a coefficient of determination of 0.71, mplying that forest management may be considered effective and in harmony with the environment. 相似文献
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Alireza Esmaeili Hojat Ahmadi Alireza Abbasi 《Archives of Agronomy and Soil Science》2017,63(2):161-170
Thermography is proposed to be an alternative non-destructive and rapid technique for the study and diagnosing of salt tolerance in plants. In a pot experiment, 30 cultivars of wheat (Triticum aestivum L.) were evaluated in terms of their leaf temperature and shoot growth and their ion distribution responses to NaCl salinity at two concentration levels: the control with electrical conductivity (EC) of 1 dS m?1 and salinity treatment with EC of 16 dS m?1 (150 mM). A completely randomized block design with factorial treatments was employed with three replications. The results indicated that thermography may accurately reflect the physiological status of salt-stressed wheat plants. The salt stress-based increase in leaf temperature of wheat cultivars grown at 150 mM NaCl reached 1.34°C compared to the control. According to the results obtained, it appears that thermography has the capability of discerning differences of salinity tolerance between the cultivars. Three salt-tolerant wheat cultivars, namely Roshan, Kharchia and Sholeh, had higher mean shoot dry matter (0.039 g plant?1) and higher mean ratio of leaf K+/Na+ (14.06) and showed lower increase in the mean leaf temperature (0.37°C) by thermography compared to the control. This was while nine salt-sensitive cultivars, namely Kavir, Ghods, Atrak, Parsi, Bahar, Pishtaz, Falat, Gaspard and Tajan, had lower mean plant dry matter production (0.027 g plant?1), lower mean ratio of K+/Na+ (9.49) and higher mean increases in leaf temperature (1.24°C). 相似文献
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Farhadi-Tooli Sakineh Ghanbari Alireza Kermani Maryam Jafarkhani Zeinalabedini Mehrshad Bettoni Jean Carlos Naji Amir Mohammad Kazemi Nooshin 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(1):31-43
European Journal of Plant Pathology - Viral diseases affect quince plant productivity and fruit quality. This study evaluated the effectiveness of droplet-vitrification cryotherapy and... 相似文献
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Alireza Seifi Dongli Gao Zheng Zheng Stefano Pavan Luigi Faino Richard G. F. Visser Anne-Marie A. Wolters Yuling Bai 《European journal of plant pathology / European Foundation for Plant Pathology》2014,138(3):641-665
Powdery mildews (PMs) cause disease in a wide range of plant species including important crops. Taking tomato as an example, here we review findings on the genetic basis and mechanisms of plant resistance to PMs. First, we present a summary of our research on tomato resistance to two PM species, with the focus on Oidium neolycopersici. We discuss the genetics of resistance to this pathogen in tomato. Then, we compare different forms of resistance mediated by different resistance genes based on molecular and cytological data. Also, we provide a comparison between these resistance genes in tomato with those in barley, Arabidopsis and wheat, in order to present a model for the genetic basis of resistance to PMs in plants. We try to accommodate these resistance mechanisms in the current model of plant innate immunity. At the end we discuss possibilities to translate these findings to practical approaches in breeding for resistance to PMs in crops. 相似文献