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51.
Sylvie Priou R. Torres Alicia Villar Liliam Gutarra F. De Mendiburu 《Potato Research》2001,44(4):349-358
Summary A sampling strategy was evaluated in the Andean highlands of Peru to optimise the detection ofRalstonia solanacearum in seed tubers harvested from symptomless crops. A sensitive and specific serological method developed at CIP was used to
detect the pathogen in latently infected tubers. Optimum sample size was evaluated for symptomless crops after analysing various
numbers of composite samples and using a binomial distribution model to calculate the detection probabilities.R. solanacearum was detected in all lots from fields with visible symptoms, so validating the detection technique. About half of the seed
lots from apparently healthy fields at altitudes of up to 3,100 m were found positive for the pathogen.R. solanacearum was detected with 99% probability in samples of 350 tubers from seed lots from symptomless crops. This number of seed tubers
could feasibly be processed in a seed-health test without incurring too high a cost for labour and materials. 相似文献
52.
A study of the ultrastructural characteristics of developing cardiac muscle cells from in vitro culture was carried out with die aid of tissues taken from the cardiogenic areas of 5 H. H. stage chick embryos. The characteristics of the developing cells were quite comparable to those developed in situ. 相似文献
53.
Villar D Nicol F Arthur JR Dicks P Cannavan A Kennedy DG Rhind SM 《Research in veterinary science》2000,68(2):119-123
The presence or absence of types I, II and III iodothyronine monodeiodinase enzymes (MDI, MDII and MDIII) and their levels of activity in the skin of goats, which were orally dosed for 60 days with 0, 1.1, 2.2, 4.4, 8.8, 17.5, or 35 mg(-1)kg liveweight day(-1)of the anti-thyroid, enzyme-inhibiting drug, propylthiouracil (PTU), were determined. Contrary to our earlier report that PTU did not influence skin MDII activity, the currect more thorough investigation (in terms of numbers of observations and the efficiency of the enzyme extraction procedure) indicated that doses of 1.1.to 17.5 mg kg(-1)liveweight induced a 2 to 3 fold increase (P = 0.01) in MDII activity. However, in three of the four goats treated with 35 mg kg(-1)group, activity was similar to that of control animals. There were no significant differences between treatments in MDIII activity but there was a trend towards lower levels of activity in the goats dosed with 17.5 and 35 mg kg(-1). It is concluded that there is significant MDII and MDIII activity in the skin of goats and that although there is none of the PTU -sensitive MDI enzyme, synthesis of T3 within the skin could nevertheless be modified through increases in MDII activity induced by lower T4 concentrations in the circulation caused by PTU. Changes in pattern of fibre moult induced by treatment with low doses of MD-inhibiting drugs may therefore be achieved through this effect. Since MDII and MDIII enzyme activity may be reduced by high doses of PTU, prolonged treatment with high doses of PTU may have adverse effects on skin tissue. 相似文献
54.
Yasmina Sanjun Amelia Gmez‐Villar Estela Nadal‐Romero Javier lvarez‐Martínez Jos Arnez María P. Serrano‐Muela Juan Manuel Rubiales Penlope Gonzlez‐Sampriz Jos M. García‐Ruiz 《Land Degradation \u0026amp; Development》2016,27(2):179-189
Channel changes are the consequence of changes in sediment yield from the slopes and in the connectivity between slopes and channels because of distinct land use and climate impacts. In this study, we investigated the characteristics and evolution of a short reach in the headwater of the Ijuez River, central–southern Pyrenees. Assessment of a series of sedimentary and geomorphic structures confirmed major changes to the valley bottom, mainly related to changes in the intensity of human activity. The oldest sedimentary structure is a terrace level located 3 to 4 m above the current alluvial plain. General deforestation, overgrazing and recurring fires in the montane belt (1100–1600 m a.s.l.) have led to increased soil erosion and connectivity, and to the triggering of debris flows that have been deposited on the fluvial terrace. Woody fragments from within the debris flows were dated using accelerator mass spectrometry 14C radiocarbon techniques (AMS), yielding ages between 100 and 115 cal years bp , which coincides with the period of maximum deforestation and human density in the Pyrenees. Depopulation and farmland abandonment since the beginning of the 20th century has resulted in generalized natural and artificial reforestation, a shrinkage of the eroded areas and a decline in connectivity between slopes and the channel. The most important consequence has been channel incision and narrowing, and the development of a sediment armour layer. Active sediment transport is continuing, although there has been a decrease in sediment yield from the slopes. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
55.
Ludovic Bonhomme Cécile Barbaroux Romain Monclus Domenico Morabito Alain Berthelot Marc Villar Erwin Dreyer Franck Brignolas 《Annals of Forest Science》2008,65(5):503-503
56.
A. Lázaro B. Villar L. Aceituno-Mata J. Tardío L. De la Rosa 《Genetic Resources and Crop Evolution》2013,60(5):1641-1654
At the mountain area close to the city of Madrid, common beans were one of the main food crops present in everyday diet until 1960. This paper describes the morphological diversity, for forty-six phenological and morphological traits, found in forty-three traditional varieties of common beans collected in this area and seven commercial varieties used as reference. That comparison suggests that common bean breeding programs have led to later varieties with a higher production of straight pods. Quantitative traits showed also similar values when compared to the Iberian common bean collection, except for the phenological and seed size characters. The phenological differences could suggest an ecological adaptation of the studied landraces to the regional environmental conditions. The different seed size averages of both collections might correspond to the scarce presence of smaller seed-type common beans in Madrid collection. All the accessions collected in the Sierra Norte of Madrid belong to any of the groups included in the Spanish core collection. Madrilenian collection is also composed by indeterminate growth habit varieties, while the presence of bush accessions is relatively uncommon. Most of the Madrilenian landraces have a remarkable fitness for green-pod consumption (42 %) and their seeds are mainly white (30 %), ovate-shape (49 %) and medium-large (40 %). The study of seed storage protein allowed to classify the landraces according to their domesticated gene pools. Most of them (72 %), with T and C phaseolin type, seem to belong to Andean germplasm, while the remaining (28 %), with S and B phaseolin type, to Mesoamerican one. The remarkable morphological diversity of common beans found in this small area is a symptom of a broad genetic base despite genetic erosion, what indicates a widespread crop in the past. Therefore, it is advisable to design agro-environmental policies to promote the production and commercialization of common bean landraces in Sierra Norte of Madrid. 相似文献
57.
Martin-Sampedro R Capanema EA Hoeger I Villar JC Rojas OJ 《Journal of agricultural and food chemistry》2011,59(16):8761-8769
Eucalyptus globulus chips were steam exploded followed by treatment with a laccase-mediator system (LMS) under different experimental conditions. Removal of hemicelluloses and, to a lesser extent, lignin was observed. Thermogravimetic analyses of whole meal obtained from chips before and after steam explosion indicated an increase in lignin degradation temperature due to lignin condensation. In contrast, application of LMS treatment caused a reduction in lignin and polysaccharide degradation temperatures. Lignins were isolated from wood samples before and after each treatment and analyzed by 2D NMR and (13)C NMR. An increase in carboxyl and phenolic hydroxyl groups and a significant decrease in β-O-4 structures were found in steam-exploded samples. The most relevant changes observed after laccase treatment were increased secondary OH and degree of condensation. 相似文献
58.
Verez-Bencomo V Fernández-Santana V Hardy E Toledo ME Rodríguez MC Heynngnezz L Rodriguez A Baly A Herrera L Izquierdo M Villar A Valdés Y Cosme K Deler ML Montane M Garcia E Ramos A Aguilar A Medina E Toraño G Sosa I Hernandez I Martínez R Muzachio A Carmenates A Costa L Cardoso F Campa C Diaz M Roy R 《Science (New York, N.Y.)》2004,305(5683):522-525
Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, however, no commercial vaccine has been available in which the key carbohydrate antigens are produced synthetically. We describe the large-scale synthesis, pharmaceutical development, and clinical evaluation of a conjugate vaccine composed of a synthetic capsular polysaccharide antigen of Haemophilus influenzae type b (Hib). The vaccine was evaluated in clinical trials in Cuba and showed long-term protective antibody titers that compared favorably to licensed products prepared with the Hib polysaccharide extracted from bacteria. This demonstrates that access to synthetic complex carbohydrate-based vaccines is feasible and provides a basis for further development of similar approaches for other human pathogens. 相似文献
59.
Chamaillard S Fichot R Vincent-Barbaroux C Bastien C Depierreux C Dreyer E Villar M Brignolas F 《Tree physiology》2011,31(10):1076-1087
The ongoing global change could be an additional threat to the establishment and the long-term survival of Populus nigra L., an emblematic European riparian species. With the general aim of gaining insights into the adaptive potential of this species, we (i) quantified variations within and among three French P. nigra populations for key physiological attributes, i.e., water-use efficiency (assessed from bulk leaf carbon isotope discrimination, Δ(13)C), growth performance and related leaf traits, (ii) examined genotype and population by environment interactions, and (iii) explored the relationship between Δ(13)C and growth. Thirty genotypes were sampled in each of three naturally established populations and grown in two different sites, Orléans (ORL) and Guémené-Penfao (GMN). In ORL, two similar plots were established and different watering regimes were applied in order to test for the drought response. Significant variations were observed for all traits within and among populations irrespective of site and watering. Trait variation was larger within than among populations. The effect of drought was neither genotype- nor population-dependent, contrary to the effect of site. The population ranking was maintained in all sites and watering regimes for the two most complex traits: Δ(13)C and growth. Moreover, these two traits were unrelated, which indicates that (i) water-use efficiency and growth are largely uncoupled in this species, and (ii) the environmental factors driving genetic structuration for Δ(13)C and growth act independently. The large variations found within populations combined with the consistent differences among populations suggest a large adaptive potential for P. nigra. 相似文献
60.
Alessandra Mirtes Marques Neves Gon?alves Aline Brito de Lima Maria Cristina da Silva Barbosa Luiz Fernando de Camargos Júlia Teixeira de Oliveira Camila de Souza Barbosa José Augusto Ferreira Perez Villar André Carvalho Costa Isabella Viana Gomes da Silva Luciana Maria Silva Fernando de Pilla Varotti Fabio Vieira dos Santos Gustavo Henrique Ribeiro Viana 《Marine drugs》2014,12(8):4361-4378
Cancer continues to be one of the most important health problems worldwide, and the identification of novel drugs and treatments to address this disease is urgent. During recent years, marine organisms have proven to be a promising source of new compounds with action against tumoral cell lines. Here, we describe the synthesis and anticancer activity of eight new 3-alkylpyridine alkaloid (3-APA) analogs in four steps and with good yields. The key step for the synthesis of these compounds is a Williamson etherification under phase-transfer conditions. We investigated the influence of the length of the alkyl chain attached to position 3 of the pyridine ring on the cytotoxicity of these compounds. Biological assays demonstrated that compounds with an alkyl chain of ten carbon atoms (4c and 5c) were the most active against two tumoral cell lines: RKO-AS-45-1 and HeLa. Micronucleus and TUNEL assays showed that both compounds are mutagenic and induce apoptosis. In addition, Compound 5c altered the cellular actin cytoskeleton in RKO-AS-45-1 cells. The results suggest that Compounds 4c and 5c may be novel prototype anticancer agents. 相似文献