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41.
Black willow (Salix nigra Marsh.) is the largest and only commercially important willow species in North America. It is a candidate for phytoremediation of polluted soils because it is fast-growing and thrives on floodplains throughout eastern USA. Our objective was to develop a protocol for the in vitro regeneration of black willow plants that could serve as target material for gene transformation. Unexpanded inflorescence explants were excised from dormant buds collected from three source trees and cultured on woody plant medium (WPM) supplemented with one of: (1) 0.1 mg l(-1) thidiazuron (TDZ); (2) 0.5 mg l(-1) 6-benzoaminopurine (BAP); or (3) 1 mg l(-1) BAP. All plant growth regulator (PGR) treatments induced direct adventitious bud formation from the genotypes. The percentage of explants producing buds ranged from 20 to 92%, depending on genotype and treatment. Although most of the TDZ-treated inflorescences produced buds, these buds failed to elongate into shoots. Buds on explants treated with BAP elongated into shoots that were easily rooted in vitro and further established in potting mix in high humidity. The PGR treatments significantly affected shoot regeneration frequency (P < 0.01). The highest shoot regeneration frequency (36%) was achieved with Genotype 3 cultured on 0.5 mg l(-1) BAP. Mean number of shoots per explant varied from one to five. The ability of black willow inflorescences to produce adventitious shoots makes them potential targets for Agrobacterium-mediated transformation with heavy-metal-resistant genes for phytoremediation. 相似文献
42.
do Amaral Ana Cludia Tenrio de Holanda Cavalcanti Maciel Marlia Machado Alexandre Reis de Oliveira Luciana Gonalves Lima Cristiano Souza da Costa Antonio Flix de Oliveira Neiva Tinti 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):875-890
European Journal of Plant Pathology - This study aimed to evaluate the potential antagonistic role of Trichoderma in the control of Fusarium isolates belonging to the Fusarium oxysporum species... 相似文献
43.
Oliveira HM Segundo MA Lima JL Grassi V Zagatto EA 《Journal of agricultural and food chemistry》2006,54(12):4136-4140
A sequential injection system for the automatic determination of glycerol in wine and beer was developed. The method is based on the rate of formation of NADH from the reaction of glycerol and NAD+ catalyzed by the enzyme glycerol dehydrogenase in solution. The determination of glycerol was performed between 0.3 and 3.0 mmol L(-1) (0.028 and 0.276 g L(-1)), and good repeatability was attained (rsd < 3.6%, n = 5) for all samples tested. The determination rate was 54 h(-1), the reagent consumption was only 0.75 micromol of NAD+ and 5.4 ng of enzyme per assay, and the waste production was 2.12 mL per assay. Results obtained for samples were in agreement with those obtained with the batch enzymatic method. 相似文献
44.
Edival Ângelo Valverde Zauza Lúcio Mauro da Silva Guimarães Nilza de Lima Pereira Sales Samuel Alves dos Santos Rafael Ferreira Alfenas Acelino Couto Alfenas 《Forest Pathology》2023,53(2):e12806
An outbreak of a new and severe disease was observed in Eucalyptus plantations of Bahia state, Brazil. An Ascomycota fungus has been frequently associated with the main symptoms of the disease namely leaf spot, branch cankers, shoot blight, defoliation, and dieback. Based on morphological characteristics, phylogenetic analysis (ITS and TEF-1α genes), and pathogenicity test on Eucalyptus plants, Pseudoplagiostoma eucalypti was identified as the causal agent of the disease. Although P. eucalytpi has been known from in Brazil since 1998, this is the first report of it causing severe disease and die-back on Eucalyptus spp. and we also record new symptoms associated with the pathogen. 相似文献
45.
46.
Pine wilt disease (PWD), recently introduced into Europe, is caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus and is a devastating illness that affects mainly pine trees. It is known that the PWN is capable of infecting other conifers; however, there is currently no information on which other plant species may be susceptible to PWD. In this study, the potential susceptibility of two common species of European forests, Picea abies and Cupressus lusitanica, to PWN was assessed through the monitoring of visual external symptoms, dimension and localization of the nematode population in stems, quantification of total chlorophyll, total soluble phenolics and lignin, at 7, 14, 21 and 28 days after inoculation. The degree of susceptibility was established through the comparison of symptoms with Pinus pinaster, a well‐known PWN host. Furthermore, the stem ultrastructure of P. abies, C. lusitanica and Pn. pinaster was analysed by scanning electron microscopy. The results suggest that P. abies and C. lusitanica are resistant to PWN, and that lignin biosynthesis in these species is affected at an early stage of the infestation. Nevertheless, P. abies seems to be a compatible host that could act as a repository for PWN. 相似文献
47.
Elcimara Cardoso Pereira Elzamara Castro Oliveira Elen Monique Oliveira Sousa Hugo Napoleão Pereira Silva Lincoln Lima Corrêa Rosa Helena Veras Mourão Marcos Tavares-Dias Lenise Vargas Flores Silva 《Journal of fish diseases》2020,43(12):1497-1504
This study investigated the acute toxicity (LC50-24 hr) effects of the essential oil of Cymbopogon citratus for adult Argulus sp. and Dolops discoidalis, before and during oviposition. In vitro acute toxicity (LC50-24 hr) was tested using 20, 40, 60, 80, 100, 120, 140 and 160 μg/L of C. citratus essential oil, and two control groups (one with cultivation tank water and one with cultivation tank water + alcohol) were used. Specimens of Argulus sp. and D. discoidalis submitted to acute toxicity were evaluated using histological procedures. The major chemical compounds of C. citratus essential oil were geranial (47.5%), neral (35.6%) and myrcene (6.7%). The LC50-24 hr for Argulus sp. adults was 67.97 μg/L, while for D. discoidalis it was 59.55 µg/L. In the oviposition of both species of argulids, maximum mortality began with treatments of 140 μg/L, while the LC50-24 hr for Argulus sp. and D. discoidalis was 83.98 μg/L and 82.48 μg/L, respectively. In both argulid species exposed to C. citratus essential oil, morphological alterations were observed only in the eyes, and they occurred in the ommatidium and rhabdomeres and were dependent on the concentration of C. citratus essential oil and the parasite species. 相似文献
48.
49.
Lima Pedro Paulo Arcanjo Narciso Luis Gustavo Alcindo Jefferson Filgueira Deschk Maurício Ciarlini Paulo Cesar dos Santos Paulo Sérgio Patto de Almeida Breno Fernando Martins 《Veterinary research communications》2022,46(1):27-35
Veterinary Research Communications - Propofol is a widely used drug in veterinary medicine to induce anesthesia; as well as the chosen compound for protocols of intravenous anesthesia. The present... 相似文献
50.
Sousa Larissa Nascimento Batista Samira Pereira Silva Samara Santos da Silva Rômulo Fylipe Sarmento Wlysse Ferreira Mota Rinaldo Aparecido Brasil Arthur Willian Lima Amaku Marcos Feitosa Thais Ferreira Vilela Vinícius Longo Ribeiro 《Veterinary research communications》2022,46(2):507-515
Veterinary Research Communications - This study aimed to describe the transmission of T. gondii in naturally infected goats in the semiarid region of northeastern Brazil, through evaluating the... 相似文献