全文获取类型
收费全文 | 2143篇 |
免费 | 120篇 |
国内免费 | 11篇 |
专业分类
林业 | 169篇 |
农学 | 120篇 |
基础科学 | 40篇 |
495篇 | |
综合类 | 154篇 |
农作物 | 298篇 |
水产渔业 | 190篇 |
畜牧兽医 | 458篇 |
园艺 | 85篇 |
植物保护 | 265篇 |
出版年
2024年 | 3篇 |
2023年 | 20篇 |
2022年 | 73篇 |
2021年 | 87篇 |
2020年 | 96篇 |
2019年 | 136篇 |
2018年 | 145篇 |
2017年 | 139篇 |
2016年 | 140篇 |
2015年 | 75篇 |
2014年 | 91篇 |
2013年 | 238篇 |
2012年 | 122篇 |
2011年 | 135篇 |
2010年 | 109篇 |
2009年 | 71篇 |
2008年 | 127篇 |
2007年 | 58篇 |
2006年 | 45篇 |
2005年 | 39篇 |
2004年 | 33篇 |
2003年 | 33篇 |
2002年 | 26篇 |
2001年 | 19篇 |
2000年 | 28篇 |
1999年 | 18篇 |
1998年 | 16篇 |
1997年 | 12篇 |
1996年 | 16篇 |
1995年 | 13篇 |
1994年 | 7篇 |
1993年 | 4篇 |
1992年 | 8篇 |
1991年 | 6篇 |
1990年 | 10篇 |
1989年 | 6篇 |
1988年 | 3篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1981年 | 4篇 |
1979年 | 5篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 4篇 |
1967年 | 3篇 |
排序方式: 共有2274条查询结果,搜索用时 15 毫秒
31.
A fusion DNA vaccine co-expressed Eimeria tenella TA4 and chicken IL-2 (chIL-2) was constructed and its efficacy against E. tenella challenge was observed. TA4 gene of E. tenella and chIL-2 gene were cloned into expression vector pcDNA3.1 and pcDNA4.0c in different forms, producing vaccines pcDNA3.1-TA4-IL-2, pcDNA3.1-TA4 and pcDNA4.0c-IL-2. The expression of aim genes in vivo was detected by RT-PCR and western blot. Animal experiment was carried out to evaluate the immune efficacy of the vaccines. Results indicated these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA3.0-TA4-IL-2 group was 192, higher than that of pcDNA3.1-TA4 group. The results suggested that TA4 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance DNA vaccine immunity. 相似文献
32.
S. Ali M. Ijaz S. H. Farooqi A. Z. Durrani M. I. Rashid A. Ghaffar A. Ali A. Rehman S. Aslam I. Khan A. Masud K. Mehmood 《Equine Veterinary Education》2021,33(2):75-83
Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses. 相似文献
33.
34.
Fernando Machado-Stredel Benedictus Freeman Daniel Jiménez-Garcia Marlon E.Cobos Claudia Nu?ez-Penichet Laura Jiménez Ed Komp Utku Perktas Ali Khalighifar Kate Ingenloff Walter Tapondjou Thilina de Silva Sumudu Fernando Luis Osorio-Olvera A.Townsend Peterson 《中国鸟类》2022,(1):1-10
Studies of biodiversity dynamics have been cast on either long(systematics)or short(ecology)time scales,leaving a gap in coverage for moderate time scales of de... 相似文献
35.
Nikbakht G Rezaii H Stear MJ Talebi MA Mahmoudzadeh H 《Veterinary journal (London, England : 1997)》2012,192(3):547-549
Ovar-DRB1 is one of the most important response genes in the major histocompatibility complex (MHC) class II region of sheep. Gene polymorphism in the second exon of Ovar-DRB1 in three different Iranian fat-tailed sheep breeds (Lori-Bakhtiari, Shaul and Zandi) was analyzed by either restriction fragment length polymorphisms (RFLP) or direct sequencing. A total of 92 Lori-Bakhtiari, 40 Shaul, and 47 Zandi sheep were examined. PCR-RFLP identified 17 genotypes in Lori-Bakhtiari sheep, 12 in Shaul sheep and 11 in Zandi sheep. Collectively, 24 different genotypes could be found for Iranian fat-tailed sheep. Using direct sequencing, seven new sequences in exon 2 of the Ovar-DRB1 gene were identified. Generalized linear modeling with a multinomial error structure showed that the sheep populations had significantly different allele frequencies. 相似文献
36.
37.
Mehdi Zarifi Sébastien Buczinski Ali Rezakhani Mohammad Reza Mokhber Dezfouli Ali Khonsha 《Journal of Veterinary Cardiology》2012,14(3):415-421
ObjectivesTo study the effect of pregnancy and lactation on echocardiographic parameters in Holstein dairy cows.AnimalsNine multiparous high milk producing (HMP) dairy cows (producing more than 40 kg of milk per day in peak production) and 9 low milk producing (LMP) cows (producing less than 30 kg or milk per day in peak production).MethodsEchocardiography was performed twice; one month before calving and two months after calving.ResultsThe heart rate of HMP cows in the early lactation period was significantly higher than in the dry period. In LMP cows, there was a significant increase in left ventricular dimension in the early lactation period as compared to the dry period, and the interventricular septum in systole (IVSs) in the dry period was significantly thicker than early lactation period. In HMP cows, there was an increase in the right ventricular diameter in systole in the early lactation period as compared to the dry period. Left ventricular and aortic dimensions in the dry period of HMP were significantly higher than those of LMP cows. When the data were corrected for body weight, comparison of values of the dry period of HMP and LMP cows showed that left ventricular volume in systole in HMP was significantly higher and that IVSs, left ventricular fractional shortening and ejection fraction were significantly lower than in LMP cows.ConclusionsThis study demonstrated that lactation influences the intracardiac dimensions. The amount of milk production can influence echocardiographic parameters in dairy cows. 相似文献
38.
Waseem Akbar Anilkumar Gowda Jeffrey E Ahrens Jason W Stelzer Robert S Brown Scott L Bollman John T Greenplate Jeffrey Gore Angus L Catchot Gus Lorenz Scott D Stewart David L Kerns Jeremy K Greene Michael D Toews David A Herbert Dominic D Reisig Gregory A Sword Peter C Ellsworth Larry D Godfrey Thomas L Clark 《Pest management science》2019,75(3):867-877
39.
40.
Journal of Plant Diseases and Protection - In the original publication, the article title was incorrectly published as ‘Impact of leave infestation by herbivorous insects on the elemental... 相似文献