全文获取类型
收费全文 | 143篇 |
免费 | 10篇 |
专业分类
林业 | 10篇 |
农学 | 3篇 |
33篇 | |
综合类 | 4篇 |
农作物 | 13篇 |
水产渔业 | 15篇 |
畜牧兽医 | 51篇 |
园艺 | 4篇 |
植物保护 | 20篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 11篇 |
2019年 | 10篇 |
2018年 | 1篇 |
2017年 | 11篇 |
2016年 | 9篇 |
2015年 | 6篇 |
2014年 | 8篇 |
2013年 | 13篇 |
2012年 | 4篇 |
2011年 | 11篇 |
2010年 | 5篇 |
2009年 | 3篇 |
2008年 | 11篇 |
2007年 | 5篇 |
2006年 | 8篇 |
2005年 | 10篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1973年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有153条查询结果,搜索用时 12 毫秒
91.
Nolan V. Chalifoux Emmanuelle M. Butty Katie D. Mauro Rachel B. Moyle Caryn M. Ehrhardt James B. Robertson Mary A. Labato Christine A. Culler Leonel A. Londoño Alessio Vigani Yu Ueda Steven E. Suter Alex M. Lynch 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):161-172
92.
Petra Sim
i
Ron Lowe Valentina Granziera Alessio Pierini Filippo Torrigiani George Lubas 《Veterinary and comparative oncology》2020,18(3):428-432
Non‐tonsillar squamous cell carcinoma (ntSCC) is a common and locally aggressive oral tumour in dogs. The treatments of choice are currently surgery and radiotherapy. Electrochemotherapy (ECT) is a local ablative anti‐tumour technique using electric pulses to enhance the intracellular diffusion of cytotoxic drugs. The aim was to retrospectively evaluate the outcome of patients with oral ntSCC treated with ECT. Twelve dogs with ntSCC were retrospectively enrolled. ECT was combined with IV bleomycin (15 000 UI/m2) alone in 11 cases and post‐surgery in 1. Parameters considered were: tumour site and size, electroporation parameters, response rate (complete remission [CR], partial remission [PR]), median survival time (MST), recurrence rate (RR), median disease‐free interval (DFI) and treatment toxicity (6‐point scale). Median tumour size was 1.65 cm (range 0.3‐8.0 cm) and the response rate was 90.9% (10/11; 8 CR and 2 PR). Two dogs underwent a second ECT. MST for dogs dead with tumour (n = 2) was 110 days and for dogs dead without tumour (n = 3) was 831 days. Among five surviving dogs, one experienced tumour recurrence and four were in CR. Results from two dogs were analysed separately. Overall RR was 27.3%. DFI and MST for dogs with recurrence were 50 and 115 days, respectively. Treatment toxicity was very low. We noticed that all dogs with tumours smaller than 1‐2 cm achieved CR without recurrence suggesting a favourable prognosis when using ECT. ECT for canine ntSCC could be considered a valid treatment option especially for smaller tumours, but a larger caseload would be needed to confirm this statement. 相似文献
93.
94.
Andre C Shih Alessio Vigani Nichole Loring Fernando G Pereira Mark Szarowicz Carsten Bandt 《Veterinary anaesthesia and analgesia》2010,37(3):215-221
ObjectiveTo compare the hemodynamic and respiratory effects of an inspiratory impedance threshold device (ITD) in anesthetized normotensive and hypotensive dogs.Study designProspective randomized study.AnimalsTen adult dogs.MethodsDogs were anesthetized with propofol followed by isoflurane. During spontaneous ventilation, tidal volume ( ), systolic (SAP), mean (MAP) and diastolic arterial blood pressure, central venous pressure, gastric PCO2 as an indicator of gastric perfusion, subcutaneous oxygen tension, subcutaneous blood flow, cardiac index (CI), systemic vascular resistance and blood lactate were monitored. To monitor respiratory compliance (RC) and resistance (ResR), animals were briefly placed on mechanical ventilation. Dogs were studied under four different conditions: 1) normotension (MAP > 60 mmHg) with and without the ITD and 2) hypotension (target MAP = 40 mmHg) with and without ITD. These four conditions were performed during one anesthetic period, allowing for stabilization of parameters for each condition. Data were analyzed by anova repeated measure mixed models.ResultsNo cardiovascular changes were detected between no ITD and ITD in the normotensive state. During hypotension, CI was higher with the ITD (5 ± 1.0 L minute?1 m?2) compared with no ITD (4 ± 1.3 L minute?1 m?2). During hypotension, SAP was increased with ITD (80 ± 14 mmHg) versus without ITD (67 ± 13 mmHg). There was an increase in ResR and decreased RC with the ITD in both normotensive and hypotensive state.Conclusion and clinical relevanceImpedance threshold device in dogs during isoflurane-induced hypotension improved CI and SAP but had negative effects on RC and ResR. 相似文献
95.
Context
Theory predicts that habitat loss and fragmentation may have drastic consequences on species’ interactions. To date, however, little empirical evidence exists on the strength of interspecific competition in shaping animal communities in fragmented landscapes.Objectives
Our aim was to measure the degree of ongoing competitive interference between species in fragmented landscapes. Our model system was the community of ground-dwelling rodents in deciduous woodlands in central Italy, composed of a habitat generalist species (Apodemus sylvaticus) and two forest specialists (Apodemus flavicollis and Myodes glareolus). Our objectives were to test whether species were segregated among forest patches and whether spatial segregation was determined by interspecific competition or habitat and resource availability.Methods
We surveyed the populations inhabiting 29 woodland patches in a highly fragmented landscape using a capture-mark-recapture protocol, capturing >4500 individuals. First we modelled species’ distribution as a function of habitat, resource availability and landscape variables. The second stage of our analyses involved measuring the response of vital rate parameters (body mass, reproduction, survival, recruitment, population density) to competitor density.Results
The relative distribution of species reflected a spatial segregation of habitat generalists and specialists according to habitat quality, cover and connectivity. Interspecific competition mainly affected individual level vital rates, whereas we found no substantial effects at the population level.Conclusions
Competitive exclusion of specialist species by generalist species was occurring. However, when compared to other factors such as habitat connectivity and resource availability, interspecific competition played a relatively minor role in shaping the studied community.96.
Kim YH Hunt MC Mancini RA Seyfert M Loughin TM Kropf DH Smith JS 《Journal of agricultural and food chemistry》2006,54(20):7856-7862
In two experiments, the relationship between metmyoglobin (MMb) reduction and lactate to pyruvate conversion with concomitant production of reduced nicotinamide adenine dinucleotide (NADH) via lactic dehydrogenase (LDH) was investigated. In experiment 1, nonenzymatic reduction of horse MMb occurred in a lactate-LDH-NAD system. Exclusion of NAD+, L-lactic acid, or LDH resulted in minimal MMb reduction. Increasing NAD+ and L-lactic acid concentrations increased reduction. In experiment 2, beef strip loins (longissimus lumborum muscle) were injected with combinations of potassium lactate, sodium tripolyphosphate, sodium chloride, and/or sodium acetate. Steaks were packaged in high-oxygen (80% oxygen/20% carbon dioxide) modified-atmosphere packaging and stored for 2-9 days and then placed in a fluorescent-lighted, open-top display case for 5 days at 1 degrees C. Enhancing loins with 2.5% potassium lactate significantly increased LDH activity, NADH concentration, MMb-reducing activity, and subsequent color stability during display. These research results support the hypothesis that enhancing beef with lactate replenishes NADH via increased LDH activity, ultimately resulting in greater meat color stability. 相似文献
97.
Lavoie JP Pasloske K Joubert P Cordeau ME Mancini J Girard Y Friesen RW Frenette R Blouin M Young RN Hickey G 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(1):175-181
Phosphodiesterase-4 (PDE 4) enzyme inhibitors have been shown to have anti-inflammatory properties in various animal disease processes and therefore could be effective drugs for the treatment of equine airway diseases. The purpose of this study was to evaluate the efficacy and adverse effects of the PDE 4 inhibitor L-826,141 in horses with heaves. In a blinded parallel design, horses with heaves exposed daily to moldy hay were given a placebo for 14 days and then administered either L-826,141 (n = 6; loading dose of 1 mg/kg IV followed by 0.5 mg/kg IV q48h) or dexamethasone (n = 6; 0.04 mg/kg IV q24h) from days 15 to 29 (study 1). Pulmonary function and bronchoalveolar (BAL) cytology were evaluated weekly from baseline (day 0) to 29 days. In study 2, horses were treated with L-826,141 (1.0 mg/kg IV q24h) for 8 days. Although ex vivo lipopolysaccharide-induced tumor necrosis factor (TNF)-alpha and LTB4 production by fresh blood were inhibited up to 90% after repeated administrations of L-826,141, this treatment failed to improve lung function. In contrast, dexamethasone (positive control) treatment resulted in significant improvement in lung mechanics and airway function in all horses. Neither drug had a significant effect on BAL total cell counts and differential cytology. Administration of the PDE 4 inhibitor L-826,141 for up to 14 days to horses with heaves was not associated with an improvement in airway function or inflammation. These findings suggest that the PDE 4 enzyme is not a key mediator of lung inflammation in heaves. 相似文献
98.
Scardillo A Pugliese M De Majo M Niutta P Pugliese A 《Veterinary therapeutics : research in applied veterinary medicine》2010,11(3):E1-E6s
The goal of glaucoma management is to reduce intraocular pressure (IOP) and maintain it at a level compatible with the health of the optic nerve. New therapies are constantly being sought. Topical instillation of levobunolol 0.5%, alone or with dorzolamide 2%, has a hypotensive effect on the IOP in healthy dogs, and levobunolol combined with dorzolamide produces a stronger hypotensive effect than the combination of timolol and dorzolamide. All animals tolerate these topical medications well with no signs of discomfort, and no ocular side effects have been observed. Levobunolol, alone or in combination with dorzolamide, induces bradycardia, as does timolol with dorzolamide. 相似文献
99.
Francesco Mancini Sara Nannarone Sandra Buratta Giuseppina Ferrara Anna Maria Stabile Matteo Vuerich Isabella Santinelli Alessandra Pistilli Elisabetta Chiaradia 《Veterinary anaesthesia and analgesia》2017,44(2):295-308
Objective
To assess the effects of xylazine and dexmedetomidine on equine chondrocytes, in vitro.Study design
Prospective, experimental study.Study material
Equine articular chondrocytes from five male horses.Methods
Chondrocytes were isolated from healthy equine articular cartilage of the metacarpo/metatarsophalangeal joints. Cell viability was assessed using the WST-8 assay by exposing chondrocytes to xylazine (0.5, 1, 2, 4, 8, 16.6, 25, 50 mg mL?1) or dexmedetomidine (0.001, 0.005, 0.01, 0.05, 0.175, 0.25 mg mL?1) for 15, 30 and 60 minutes. Based on the results of these tests, cells were treated with xylazine (1, 4, 25 mg mL?1) or dexmedetomidine (0.05, 0.175, 0.25 mg mL?1) for 15 minutes to further evaluate: cell viability by neutral red uptake; cell membrane integrity by lactate dehydrogenase release and by fluorescence microscopy with Hoechst 33342 and propidium iodide (PI), and apoptosis by flow cytometry using double staining with annexin V-fluorescein isothiocyanate/PI and by cell morphology.Results
Both drugs reduced cell viability in a dose-dependent manner. Specifically, all xylazine concentrations, except 0.5 mg mL?1 and 1 mg mL?1, significantly reduced cell viability, whereas the effects of dexmedetomidine were evident only at 0.175 mg mL?1 and 0.25 mg mL?1. The highest concentrations of xylazine (25 mg mL?1) and dexmedetomidine (0.25 mg mL?1) caused loss of membrane integrity. Cell morphology and flow cytometry analyses demonstrated signs of late apoptosis in xylazine-treated cells, and signs of late apoptosis and necrosis in dexmedetomidine-treated cells.Conclusions and clinical relevance
This study offers new insights into the potential chondrotoxicity induced by dexmedetomidine and xylazine. Therefore, the intra-articular administration of α2-agonists should be conducted with care, especially for doses of ≥ 4 mg mL?1 of xylazine and 0.175 mg mL?1 and 0.25 mg mL?1 of dexmedetomidine. 相似文献100.
Natalia Sevane Hubert Levéziel Geoffrey R Nute Carlos Sa?udo Alessio Valentini John Williams Susana Dunner 《畜牧与生物技术杂志(英文版)》2014,5(1):20