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91.
Caboni P Cabras M Angioni A Russo M Cabras P 《Journal of agricultural and food chemistry》2002,50(12):3491-3494
In this work an HPLC method for the determination of azadirachtin residues on olives was developed, and the field degradation kinetics of the pesticide was studied. In field trials the active ingredient (a.i.) decay had a half-life time of 0.8 days, which was too short to show a good efficacy of treatment. The mechanism of disappearance of the pesticide studied with model systems showed that it was unrelated to evaporation, thermodegradation, and co-distillation, but it was related to photodegradation. The high photodegradation rate of commercial formulations calls for the need to test different formulates in order to increase the persistence of the residue and thus the pesticide's efficacy. 相似文献
92.
Fernández-Surumay G Osweiler GD Yaeger MJ Rottinghaus GE Hendrich S Buckley LK Murphy PA 《Journal of agricultural and food chemistry》2005,53(10):4264-4271
The effects of fumonisin B-glucose reaction products in swine diets was examined. Pigs were fed diets containing 528 micromol of total fumonisin B/kg (FB), 528 micromol of total FB-glucose adducts/kg (FB-G, 122 micromol of unreacted FB/kg), or 0 micromol of total FB/kg for 15 days to test the efficacy of the FB-G reaction products in detoxifying FB. Weight gain in FB pigs was lower than in FB-G or controls, which was correlated with feed intake reduction in FB pigs. Serum aspartate aminotransferase, gamma-glutamyltransferase, and total bilirubin in FB pigs were higher than in FB-G or control pigs. Serum sphinganine/shingosine ratios in FB pigs were higher than in FB-G or control pigs. Microscopic examination of tissues from FB pigs showed generalized liver necrosis and apoptosis with marked cellular pleomorphism and disorganized hepatic cords. The liver and kidneys in the FB-G group appeared to be normal. Tissues of controls were free of lesions. Results suggest that dietary FB-G products are less toxic to swine and may provide an detoxification approach in instances of widespread FB grain contamination (p < 0.05). 相似文献
93.
Fernández MJ Oliva J Barba A Cámara MA 《Journal of agricultural and food chemistry》2005,53(3):804-811
The evolution of residual levels of four fungicides (cyprodinil, fludioxonil, pyrimethanil, and quinoxyfen) during the elaboration of three types of wine with maceration (traditional red wine, carbonic maceration red wine, and red wine of long maceration and prefermentation at low temperature) and two types of wine without maceration (rose and white) has been studied. The disappearance curves of each fungicide have been analyzed during the period of each winemaking process (21 days) and during the different enological steps involved in the elaborations. The residual levels of fludioxonil reduce most quickly during the winemaking processes without maceration, whereas the decrease in levels of pyrimethanil was the slowest in practically all cases (with and without maceration). During carbonic maceration winemaking, the decay constant of cyprodinil was greater than that of the other pesticides in all assays (time and steps). 相似文献
94.
Garde S Carbonell M Fernández-García E Medina M Nuñez M 《Journal of agricultural and food chemistry》2002,50(23):6752-6757
The effect of the addition of Lactococcus lactis subsp. lactis INIA 415, a strain harboring the structural genes of nisin Z and lacticin 481, on the formation of volatile compounds in Hispánico cheese manufactured with a mesophilic starter or with the mesophilic starter and a thermophilic starter was investigated. Addition of bacteriocin-producing L. lactis subsp. lactis INIA 415 to milk enhanced the formation of 2-methyl-propanal, 2-methylbutanal, 3-methylbutanal, 2-methyl-1-propanol, 3-methyl-1-butanol, 1-octanol, 2-butanone, and 2,3-butanedione. On the other hand, addition of thermophilic starter enhanced the formation of acetaldehyde, ethanol, 3-methyl-2-buten-1-ol, ethyl butanoate, ethyl hexanoate, 2-butanone, and 2,3-butanedione in Hispánico cheese. Stepwise discriminant analysis using the relative abundances of volatile compounds classified cheeses by type of starter, with function 1 related to thermophilic starter and function 2 to bacteriocin producer. 相似文献
95.
Mohamed A. Tarawally Hanoi Medina M. E. Frmeta C. Alberto Itza 《Soil & Tillage Research》2004,76(2):95-103
The level of compaction induced on cultivated fields through trafficking is strongly influenced by the prevailing soil-water status and, depending on the attendant soil degradation, vital soil hydraulic processes could be affected. Therefore, understanding the relationship between field soil-water status and the corresponding level of induced compaction for a given load is considered an imperative step toward a better control of the occurrence of traffic-induced field soil compaction. Pore size distribution, a fundamental and highly degradable soil property, was measured in a Rhodic Ferralsol, the most productive and extensively distributed soil in Western Cuba, to study the effects of three levels of soil compaction on soil water characteristic parameters. Soil bulk density and cone penetration index were used to measure compaction levels established by seven passes of a 10 Mg tractor at three soil-water statuses corresponding to the plastic (Fs), friable (Fc) and relatively dry soil (Ds) consistency states. Pore size distribution calculated from soil water characteristic curves was classified into three pore size categories on the basis of their hydraulic functioning: >50 μm (f>50 μm), 50–0.5 μm (f50–0.5 μm) and <0.5 μm (f<0.5 μm). The greatest compaction levels were attained in the Fs and Fc soil water treatments, and a significant contribution to compaction was attributed to the existing soil water states under which the soil compaction was accomplished. Average cone index (CI) values in the range of 2.93–3.70 MPa reflected the accumulation of f<0.5 μm pores, and incurred severe reductions in the volume of f>50 μm pores in the Fs and Fc treatments, while an average CI value of 1.69 MPa indicated increments in the volume of f50–0.5 μm in the Ds treatment. Despite the differential effects of soil compaction on the distribution of the different pore size categories, soil total porosity (fTotal) was not effective in reflecting treatment effects. Soil water desorption at the soil water potentials evaluated (0.0 to −15,000 cm H2O) was adversely affected in the f<0.5 μm dominated treatments; strong soil water retention was observed with the predominance of f<0.5 μm, as was confirmed by the high water content at plant wilting point. Based on these findings, the use of field capacity water content as the upper limit of plant available soil water was therefore considered inappropriate for compacted soils. 相似文献
96.
The effect of orientation, location, year, and pruning treatment on mean acorn mass in two open woodland experimental locations (Huelva, South-Western Spain) of Holm Oak (Quercus ilex L. subsp. ballota [Desf.] Samp.) was analyzed during 2001–2006. Data were analyzed by using a mixed linear model, where the covariates were tree size parameters (circumference at 80 cm height, crown size), topographic position (wet index and flow length), intraspecific competition, total acorn crops per tree (weight and number), and climatic parameters. Average estimated acorn mass was 3.17 g acorn?1 in the Calañas location and 4.12 g acorn?1 in the San Bartolomé location, with significant differences between locations, among individual trees and across years. Acorns from the south sides of trees were significantly heavier than those at other positions. The effect of pruning treatment and the interactions location × treatment and treatment × year were not significant. Our study provides no evidence that pruning affects acorn mass. None of the analyzed covariates related to tree size, topographic position and intraspecific competition explained the difference among trees, suggesting that heritable factors play an important role. Specific climatic parameters during September, the most important month for acorn growth, may explain the effects of year and location × year on acorn mass. In addition, there appeared to be a “trade-off” between acorn mass and number of acorns. 相似文献
97.
Noeleen T. McDonald Miguel A. Dionisio Fernández David P. Wall 《Communications in Soil Science and Plant Analysis》2016,47(22):112-121
ABSTRACTA reliable and practical test that can provide timely measurements of the levels of mineralizable nitrogen (MN) in soil is critical for improving the accuracy of N fertilizer applications for grassland and crops. The Illinois soil N test (ISNT) is considered to be a good estimate of MN, once soils are grouped according to soil characteristics such as the drainage type and sampling depth. To date, development and evaluation of the ISNT method has been conducted using arable soils mainly in North America where, in general, soils have lower levels of soil organic matter (SOM) compared to temperate grassland soils. We evaluated the effects of two pre-treatment soil aggregate sizes of <1 mm and <2 mm on the yield and recovery of MN (1) across temperate grassland soil types, and (2) across a 6-h interval diffusion period. No significant difference existed in the concentrations of ISNT-N between the two soil aggregate sizes of each soil type. For both aggregate sample sizes, the recovery of spiked amino sugar-N glucosamine from a temperate grassland soil was generally linear until hour 5, after which the quantities of recovered N diminished. Although N recovery after 6 h of diffusion at 50°C (±1°C) was less than 100% in both aggregate size samples, the response models indicated that the standard ISNT protocol using a 5-h diffusion period is appropriate for temperate grassland soils. The incomplete recovery of N in these mineral soils suggested that the protocol could be further optimized for temperate soils with high organic matter content and additional evaluation of the temperature during diffusion within an enclosed environment may be required using N (spiked glucosamine-N) recovery studies. 相似文献
98.
Jiménez A Rodríguez R Fernández-Caro I Guillén R Fernández-Bolaños J Heredia A 《Journal of agricultural and food chemistry》2001,49(1):409-415
Olive fruits at three stages of ripening (green, cherry, and black) have been studied. After cell wall isolation, the compositions of the cell wall and that of the phosphate-soluble polysaccharides were determined. In cell walls, decreases in arabinose, xylose, glucose, and uronic acid levels were observed, together with a slight increase in mannose on ripening. At the beginning of ripening, fragments of pectic polymers were the major constituents of the phosphate-soluble fraction, with the hemicellulosic ones increasing toward the end of the process. The molecular weight of the fragments solubilized was approximately 6 kDa. After cell wall fractionation, the pectic polysaccharides soluble in imidazole and sodium carbonate were also studied. In both fractions, between the green and cherry stages of ripening, a significant loss of homogalacturonans took place. Between the cherry and black stages of ripening, rhamnogalacturonan side chains were also released in addition to homogalacturonans. In any of the pectic fractions, changes in apparent molecular weight were quantified. 相似文献
99.
Controlled release of carbofuran from an alginate-bentonite formulation: water release kinetics and soil mobility 总被引:4,自引:0,他引:4
Fernández-Pérez M Villafranca-Sánchez M González-Pradas E Martinez-López F Flores-Céspedes F 《Journal of agricultural and food chemistry》2000,48(3):938-943
The insecticide-nematicide carbofuran was incorporated in alginate-based granules to obtain controlled-release (CR) properties. The basic formulation [sodium alginate (1.61%)-carbofuran (0. 59%)-water] was modified by addition of sorbents. The effect on carbofuran release rate, caused by the incorporation of natural and acid-treated bentonite (0.5 and 1.0 M H(2)SO(4)) in alginate formulation, was studied by immersion of the granules in water under shaking. The time taken for 50% of the active ingredient to be released into water, t(50), was longer for those formulations containing natural bentonite (6.1 h) or acid-treated bentonite (9.0 and 11.7 h for 0.5 and 1.0 M H(2)SO(4) treatments, respectively) than for the preparation without bentonite (4.7 h). It appears from the results that the release of carbofuran from the various formulations is controlled by a diffusion mechanism according to the n values obtained, which were close to 0.5 in all cases. The mobility of carbofuran from alginate-based CR formulations was investigated by using soil columns packed with a clay soil (53% clay and 0.08% organic matter). Two alginate-based CR formulations containing natural bentonite or acid-treated bentonite (0.5 M H(2)SO(4)) were compared to technical grade carbofuran. The use of alginate-based CR formulations resulted in a reduction of the leached amount of carbofuran compared with the total amount of pesticide leached using the technical product (50 and 75% for CR granules containing natural and acid-treated bentonite, respectively). Alginate-bentonite CR formulations might be efficient systems for reducing carbofuran leaching in clay soils, which would reduce the risk of groundwater pollution. 相似文献
100.
Valls F Checa MA Fernández-Muiño MA Sancho MT 《Journal of agricultural and food chemistry》1999,47(1):170-173
A reliable and rapid high-performance liquid chromatography (HPLC) method has been set up for the determination of total thiamin in difficult sample matrices such as cooked sausages. Different hydrolysis conditions and enzymes were tested to release the vitamin from its phosphate ester. The best data in the enzymatic digestion were obtained by incubating the samples with 6% clara-diastase at 50 degrees C for 3 h. After oxidation of thiamin to thiochrome, the sample extracts were purified by using a C(18) Sep-Pak cartridge. Final determination was performed by reversed-phase HPLC with fluorescence detector (excitation 360 nm, emission 430 nm), on a low-cost 25 cm x 4 mm i.d. Spherisorb C(8) cartridge using a mixture of 5 mM phosphate buffer pH 7.0 and acetonitrile (70:30, v/v) as mobile phase. Precision of the method was 1.5% (within a day) and 5. 2% (between days). The detection limit was 0.015 mg/100 g. All the recoveries from the different cooked sausages were better than 90% of thiamin hydrochloride added to samples of meats. In the samples analyzed, the mean value for thiamin was between 0.039 and 0.508 mg/100 g fresh weight. 相似文献