Cellular injury and lipid peroxidation induced by hexavalent chromium in isolated rat hepatocytes

In order to elucidate the relationship between cellular injury and lipid peroxidation induced by hexavalent chromium (CrVI), isolated rat hepatocytes treated with any one of scavengers of active oxygen species, antioxidants or antichromium agent were incubated with K2Cr2O7 as CrVI (1 mM Cr). After the incubation, the development of lipid peroxidation was determined as thiobarbituric acid (TBA)-reacting materials in total lipid extracts from the incubated hepatocytes. Cellular injury was observed as a leakage of lactate dehydrogenase (LDH) from hepatocytes into incubation medium. The contents of reduced glutathione (GSH) in hepatocytes were also assessed. Results obtained were as follows: (1) CrVI facilitated lipid peroxidation in isolated hepatocytes after 20 min of incubation. On the other hand, the cellular injury induced by CrVI was barely observed even after 60 min of incubation. (2) The CrVI-induced lipid peroxidation was inhibited by catalase and mannitol as scavengers of active oxygen species, or N,N'-diphenyl-p-phenylenediamine and alpha-tocopherol as antioxidants. However the cytotoxicity of CrVI could not be prevented by these chemicals. (3) CrVI depleted the contents of intracellular GSH and diminished the activities of glutathione reductase (GR) and glutathione-S-transferase (GST) except glutathione peroxidase. (4) The scavengers of active oxygen species and the antioxidants could not prevent the depletion of intracellular GSH induced by CrVI. (6) Ascorbic acid, antichromium agent, prevented all of the lipid peroxidation, the cellular injury and intracellular GSH depletion induced by CrVI.(ABSTRACT TRUNCATED AT 250 WORDS)     

Efficacy of nemadectin, a new broad-spectrum endectocide, against natural infections of canine gastrointestinal helminths

Nemadectin, a new broad-spectrum endectocide, was highly efficacious against natural infections of all the major canine gastrointestinal helminths. At single oral dosages of 0.2-0.4 mg kg-1 body weight (BW), a liquid formulation administered in gelatin capsules was 100% effective in eliminating natural infections of Toxocara canis, Toxascaris leonina, Ancylostoma caninum and Uncinaria stenocephala. Tablets (267 mg) containing 4.6% nemadectin given at a rate of 1/3 tablet per 20 kg BW (0.2 mg nemadectin kg-1) were 100% active against T. canis, A. caninum and U. stenocephala. With both formulations, an increase in the dose rate to 0.6-0.8 mg kg-1 BW resulted in 99-100% elimination of Trichuris vulpis infections. No adverse reactions were observed in any of the treated dogs.     

Reproductive performance of three fat-tailed breeds of sheep mated to native,Corriedale and Targhee rams

Summary Reproduction and lamb production of three fat-tailed, carpet-wool Iranian breeds of sheep; Karakul (130 ewes), Mehraban (103) and Baluchi (119) were studied. The ewes were mated either as purebreds or with Corriedale or Targhee rams. The overall least-squares means for ewes mated (showed oestrus) or ewes exposed, ewes lambed or ewes mated, lambs born of ewes lambing (prolificacy), lambs born alive of total lambs born, lambs weaned of live lambs born, lambs weaned (75 days of age) of ewes mated, kg lamb weaned per ewe mated and kg lamb weaned per 100 kg ewe mated were 98.3%, 93.5%, 103.1%, 94.9%, 97.8%, 90.0%, 18.2 kg and 38.4 kg respectively. The native breeds were not different for any of the traits studied except for prolificacy which was higher in Mehraban than in Karakul. Purebred matings did not differ from the crossbred matings for any of the traits concerning the number of lambs but crossbreeding improved kg lamb weaned per ewe mated (9.2%, P=0.08) and kg lamb weaned per 100 kg ewe mated (10.0%, P<0.05). Among the three native breeds Karakul showed the largest improvement when crossed with the exotic rams for the traits concerning kg lambs weaned. Corriedale and Targhee rams had similar effects on all the traits studied. A lower percentage of virgin ewes showed oestrus, had a lower twinning rate and productivity compared with the mature ones. Sex was not a significant source of variation in lamb livability.

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Desempeño Reproductivo De Tres Razas Cola Gruesa De Ovejas Apareadas Con Moruecos Nativos, Corridale Y Targhee

Resumen Se estudiaron la reproducción y producción de corderos iraníes de tres razas fat-tailed (Cola gruesa o grasosa) productores de lana para carpetas: Karakul (130 ovejas), Mehraban (103) y Baluchi (119). Las ovejas fueron apareadas como puras, o con moruecos Corridale o Targhee. Las medias mínimas cuadradas para ovejas apareadas (que mostraron estro) de ovejas expuestas, ovejas paridas de ovejas apareadas, corderos nacidos de ovejas paridas (prolificidad), corderos nacidos vivos del total de corderos nacidos, corderos destetados de corderos nacidos vivos, corderos destetados (75 días de edad) de ovejas apareadas, kg de cordero destetado por oveja apareada y kg de cordero destetado por 100 kg de oveja apareada, fueron: 98.3%, 93.5%, 103.1%, 94.9%, 97.8%, 90.0%, 18.2 kg y 38.4 kg respectivamente. Las razas nativas no fueron diferentes en ninguna de las características estudiadas, excepto por le prolificidad la cual fue mayor en Mehraban, que en Karakul. El apareamiento de razas puras, no difirió de los apareamientos cruzados para cualquiera de las características estudiadas concernientes con el número de corderos, pero el cruzamiento mejoró los kg. de cordero destetado por oveja apareada (9.2%,p<0.08) y kg de cordero destetado por 100 kg de oveja apareada (10.0%,P<0.05). De las tres razas nativas, la Karakul fue superior cuando se cruzó con las razas exóticas en lo concerniente a las características kg de cordero destetado. Las razas Corriedale y Targhee tuvieron efectos similares en todas las características estudiadas. Un bajo porcentaje de ovejas vírgenes ciclaron regularmente, tuvieron un porcentaje bajo de mellizos y productividad en general, comparada con las ovejas maduras. El sexo no fue una fuente significativa de variabilidad, en la supervivencia de los corderos.

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Performances De Reproduction De Trois Races De Moutons À Queue Grasse Accouplées À Des Locales, Corriedale Et Targhee

Résumé La reproduction et la production d'agneaux de 3 races iranienes à queue grasse élevées pour la production de laine à tapis: karakul (130 brebis), Mehraban (103) et Baluchi (119) furent étudiées; Les brebis furent accouplées soit en race pure soit avec des béliers Corriedale ou Targhee; Les moyennes estimées par la méthode des moindres carrés de l'ensemble des brebis accouplées (oestrus visible) par rapport aux brebis exposées; des brebis agnelant par rapport aux brebis accouplées, des agneaux nés par rapport aux brebis agnelant (prolificité); des agneaux nés vivants sur les nés totaux, des agneaux sevrés sur les nés vivants, des agneaux sevrés par brebis accouplée, et du kg d'agneau sevré par 100 kg de brebis accouplées étaient 98,3%; 93,5%, 103,1%, 94,9%, 97,8%, 90,0%, 18; 2 kg et 38,4 kg respectivement. Les races natives n'étaient pas différentes pour les autres caractères étudiés excepté pour la prolificité qui était plus élevée en Mehraban qu'en Karakul. Les accouplements en race pure ne différaient pas des croisements pour tous les caractères concernant le nombre d'agneaux mais le croisement améliorait les kg d'agneaux sevrés par 100 kg de brebis accouplée (9,2%P=0,08) et les kg sevrés par 100 kg de brebis accouplée (10,0%P<0,05). Parmi les 3 races natives, la Karakul montrait l'amélioration la plus grande lorsque croisée avec les béliers exotiques pour les caractères concernant les kg d'agneaux sevrés. Les béliers Corriedale et Targhee avaient des effets similaires sur tous les caractères étudiés. Un plus faible pourcentage de brebis vierges à oestrus visible, avait un plus faible taux de jumeaux et de productivité comparé aux brebis matures. Le sexe n'était pas une source significative de variation dans la viabilité des agneaux.

     

The prevalence and identity of Sarcocystis cysts in cattle in Belgium

Muscle tissue from the oesophagus, diaphragm and heart of 100 cattle slaughtered in Belgium was examined for Sarcocystis infection by microscopic examination of tissue and artificial digestion. Intact sarcocysts or cystozoietes were recovered from 97% of the cattle examined. There was a difference in sensitivity between the method (digestion or histology) used and the muscle processed. The digestion of the oesophagus muscle resulted in the highest number of positive animals whereas the heart muscles contained most cysts during histological examination. Thin-walled cysts were recovered from all positive animals especially in the heart and they were indistinguishable from those of S. cruzi. Thick-walled cysts were recovered from 56% of animals but these could not be identified as S. hirsuta and/or S. hominis on morphological grounds. A correlation between pathological changes and the infection grade could not be proved.     

白枕鹤精液冷冻技术的研究

白枕鹤（ＧＲＵＳＶ　ＩＰＩＯ）羽毛青灰，红面，体型健美优雅，具有很高的观赏价值，已被国家列为二级保护动物。对白枕鹤类的人工授精、孵化、育雏以及冷冻精液技术的研究，可促进人工饲养条件下鹤类的增殖和种群的繁衍。本研究采用Ｂｅｌｔｓｖｉｌｌｅ稀释液做为基础液，４—６％ＤＭＳＯ做为冷冻保护剂，按常规冷冻法冻精配种受胎率达４０％，此项研究填补了我国在鹤类精液冷冻技术方面的空白。     

Comparative infectivity for axenic and specific-pathogen-free chickens of O2 Escherichia coli strains with or without virulence factors

Adhesion to epithelial respiratory cells, iron acquisition, and production of K1 polysaccharide capsules have been proposed as potential virulence factors of avian Escherichia coli. These factors were studied by inoculating groups of axenic or specific-pathogen-free (SPF) chickens intratracheally with O2 E. coli strains after previous challenge with a wild strain of infectious bronchitis virus (IBV). In all experiments, the association between IBV and an E. coli strain endowed with the three virulence factors previously mentioned resulted in the most severe pathological effects, as measured by mortality, weight gains, lesions, and reisolation of E. coli from internal organs. An E. coli strain devoid of virulence factors was able only to induce mild pathological effects restricted to the respiratory tract when combined with IBV. Both E. coli strains were more invasive in axenic chickens than in SPF chickens. These results confirm the probable involvement of the three factors studied in the pathogenic properties of avian E. coli. This model can be used to assess the role of virulence factors, by comparing pairs of positive and negative isogenic strains.     

Alteration of nitrogen metabolism by alpha-ketoglutarate administration in growing lambs fed high nonprotein nitrogen-containing diets

Two completely randomized design experiments were conducted, using either 10, 38-kg (Exp. 1) or 10, 26-kg (Exp. 2) Hampshire x Western wether lambs, to study the effects of alpha-ketoglutarate (AKG) administration on N metabolism. Lambs were fed 890 and 885 g DM/d in Exp. 1 and 2, respectively, of corn-cottonseed hull basal diets with urea added to attain CP levels of 10.6 and 10.5% in Exp. 1 and 2, respectively. Experiments consisted of 10 d of adaptation followed by 7 d of infusion and excreta collection. Lambs were infused continuously either i.v. (Exp. 1) or abomasally (Exp. 2) with control solutions (CON) or solutions containing 41.3 g AKG/d (AKG). In Exp. 1, fecal and urinary N excretion and N retention were not affected (P greater than .10) by treatment. Compared to CON in Exp. 2, AKG infusion increased (P less than .10) fecal N output (6.6 vs 5.9 g N/d) but did not affect (P greater than .10) the amount of N retained (4.4 vs 3.5 g N/d). Compared to CON, AKG increased (P less than .10) urinary NH3 N excretion in Exp. 1. Serum urea N was lower (P greater than .10) for AKG than for CON in Exp. 1 but was not affected (P greater than .10) by treatment in Exp. 2. In Exp. 1, AKG appeared to reduce activities of several serum enzymes that function in amino N metabolism. In Exp. 1, compared to CON, AKG decreased (P less than .10) aspartate but increased (P less than .10) asparagine in serum.(ABSTRACT TRUNCATED AT 250 WORDS)