龙眼胚性愈伤组织2个乙烯受体基因的克隆及序列分析

以龙眼胚性愈伤组织为材料,采用同源克隆和RACE方法分离得到两个乙烯受体基因的cDNA序列,即DL-ETR1和DL-ERS1,并在GenBank登录(检索号分别为FJ513322和FJ513323)。DL-ETR1全长为2611bp,包含长2223bp的完整的开放阅读框(ORF)以及388bp的3-UTR,3'poly(A)尾长24bp;DL-ERS1全长为2259bp,包含一个1929bp的ORF及330bp的3-UTR,3'poly(A)尾长17bp。两者根据核苷酸序列推测的蛋白质具有61.62%的同源性,在N端的同源性较C端高,DL-ERS1比DL-ETR1少104个氨基酸,DL-ERS1的蛋白在C端缺乏信号接受域,HisKA、ATPase_c以及GAF是它们共有的结构域,同属ETR1亚类乙烯受体。     

珍珠番石榴和红拔的引种试种

番石榴属桃金娘科番石榴属热带、亚热带果树，富含维生素C，营养价值高，深受人们的喜爱。在我省农业产业结构调整中，番石榴的栽培日益受到人们的重视。我所于2001年春季从漳州市长泰县引进台湾珍珠番石榴及红拔，试验园土壤基本概况为：PH6．95，有机质12．1克／公斤，水解氮32．6毫克／公斤（下同），速效磷38．9，速效钾51．0，交换态钙883．7，交换态镁84．3，年降雨量1559．5毫米，年平均气温20．9℃。土壤基本养分状况良好。现将其生长结果表现及栽培技术介绍如下。     

植物开花的激素调节（二）（续一）

红藤[Chenopodium rubrum]对于这种植物,生长素的作用位置似乎集中在顶端分生组织(Krekule和Privratsky,1974).光诱导引起侧生分生组织的有丝分裂活动增强,而对苗端施用生长素则使它受到抑制(Seidlova和Khatoon,1976).因而,生长素促进植物花的发端,可能是抑制了腋下的生长而刺激了苗端的生长.Seidlova(1980)报道,生长素对植物开花     

福州花店业市场营销状况调研

以福州市花店行业为研究对象,以市场营销学为理论基础,运用分层抽样法、访问法、结构分析法、比较分析法等市场调研方法,对福州花店的消费者特点、花店营销现状进行了调查分析,并提出了相应的建议,以期对福州花店业的发展有所帮助,为相关科研人员以及从业人员提供参考.     

蓝光补光对福建金线莲生长及药用成分的影响

以福建尖叶品种金线莲种植苗为材料,用LED蓝光进行不同光周期补光试验,研究补充光照对金线莲生长及药用成分积累的影响。结果表明:补充蓝光光照对金线莲生长有积极的促进作用,其植株叶片数、茎粗、根长、鲜重、干重等指标有不同程度地增长;尤其是8 h/d处理,上述指标均显著高于对照。补充蓝光光照使植株总糖、多糖含量略有增长,但差异不显著;3个处理植株总多酚含量均高于对照,且8 h/d处理植株增幅显著;3个补光处理总黄酮含量均显著高于对照。      

大花蕙兰苗期叶面积的估算方法

采用叶长叶宽常数的方法估算大花蕙兰苗期叶面积,其中常数K由最小极差平方和法求得。结果表明:叶片长分成10-20,20-30,30-40cm3个等级,其常数分别为0.840,0.783,0.797。若不分等级,其共同常数为0.797。通过K值方差同质性测验,对叶估算面积与实际面积之间的相关分析和协方差分析进行检验。统计分析结果证明:X(估算面积)=0.797×L(叶长)×W(叶宽)公式适用于叶长40cm以下苗期的叶面积估算,建立此方法测定叶片生长速率具有不破坏性,且迅速、准确等优点,可在大花蕙兰生产上应用。     

失水胁迫对三角梅插穗叶片光合作用的影响

探讨了失水胁迫对三角梅插穗叶片光合作用的影响。结果表明:扦插前随着离体时间的延长,插穗叶片水势逐渐降低,失水胁迫逐渐加重,扦插后插穗叶片的光合能力明显下降。离体时间超过2 h、插穗失水率大于8.9%时,叶片光合速率的下降主要受非气孔因素限制。     

蝴蝶兰组培苗移植技术研究

对蝴蝶兰组培苗的移植技术进行研究,结果表明:室内炼苗4d+室外炼苗4d这种炼苗方式,能使幼苗成活率达94.0%;用水苔、椰丝作为移栽基质,幼苗成活率分别达到98%、96%,而且幼苗长势良好;对出瓶后的幼苗以及基质水苔进行消毒,都能极显著提高幼苗的成活率。     

龙眼TIR1和ABP1基因的克隆及其在体胚发生过程中的表达分析

AIM: To investigate the expression and the role of histone deacetylase 8 (HDAC8) in cardiac hypertrophy and the effects of valproic acid sodium (VPA, a histone deacetylase inhibitor) on the expression of HDAC8 and cardiac hypertrophy in two-kidney two-clip (2K2C) renovascular hypertensive rats. METHODS: Male SD rats were randomly divided into sham operation group, 2K2C group, high dose VPA (400 mg穔g^-1穌^-1) treatment group, low dose VPA (200 mg穔g^-1穌^-1) treatment group and candesartan (10 mg穔g^-1穌^-1) treatment group.Four weeks after surgery,the rats were intraperitoneally injected with VPA for 4 weeks. Sham operation and 2K2C rats were given vehicle for 4 weeks. All animals were sacrificed 8 weeks after surgery. The ratio of left ventricular weight to body weight (LVW/BW) was calculated and pathological changes of the myocardium were observed with HE staining. The mRNA expression of atrial natriuretic factor (ANF) and HDAC8 was examined by RT-PCR. The protein level of HDAC8 was also measured by Western blotting analysis. RESULTS: Compared with the control rats, the mRNA and protein expression of HDAC8 significantly increased in the myocardium in 2K2C rats while the mRNA and protein expression of HDAC8 was significantly decreased by VPA treatment in 2K2C rats. The mass index (as measured by LVW/BW) and cardiomyocyte cross areas were markedly increased and myocardial fibers were disordered in 2K2C rats, but these parameters were markedly reversed after treated with VPA for 4 weeks, indicating that VPA attenuated cardiac hypertrophy. Moreover, VPA also decreased the mRNA expression of ANF. CONCLUSION: HDAC8 may play an important role in cardiac hypertrophy in 2K2C renovascular hypertensive rats. VPA inhibits the expression of HDAC8 and prevents the development of cardiac hypertrophy.