Protection conferred against viral nervous necrosis by simultaneous inoculation of aquabirnavirus and inactivated betanodavirus in the sevenband grouper, Epinephelus septemfasciatus (Thunberg)

An aquabirnavirus (ABV) and a formalin-inactivated betanodavirus [redspotted grouper nervous necrosis virus (RGNNV)] were investigated for their potential to prevent RGNNV-induced viral nervous necrosis (VNN) in the sevenband grouper, Epinephelus septemfasciatus (Thunberg). Three groups of fish were injected intramuscularly with ABV, intraperitoneally with inactivated RGNNV (iRGNNV) or with both ABV and iRGNNV. At 3, 7, 14, 21 and 28 days post-injection (p.i.), fish were challenged by intramuscular injection of RGNNV. Control fish, which received neither ABV nor iRGNNV, showed high mortalities in all RGNNV challenges. Fish that received only ABV exhibited relative percent survival (RPS) of >60 against RGNNV challenges at 3, 7, 14 and 21 days p.i., but not at 28 days p.i., while fish that received only iRGNNV showed significantly higher protection against RGNNV challenges only at 21 and 28 days p.i. In contrast, fish that received both ABV and iRGNNV showed 60 or higher RPS against all RGNNV challenges. Fish inoculated with iRGNNV with or without ABV exhibited similar high titres of neutralizing antibodies to RGNNV at 14, 21 and 28 days p.i. These results indicate that combined inoculation with iRGNNV and ABV conferred both rapid non-specific and delayed specific protection against VNN.     

Quantitative structure—activity studies of insect growth regulators. XI. Stimulation and inhibition of N-acetylglucosamine incorporation in a cultured integument system by substituted N-tert-butyl-N,N′-dibenzoylhydrazines

The ability to stimulate N-acetylglucosamine (GluNAc) incorporation in-vitro of a number of N-tert-butyl-N,N′-dibenzoylhydrazines having various substituents on both phenyl rings was measured in cultured integument excised from the rice stem borer (Chilo suppressalis Walker). The relationship between in-vitro and larvicidal potency was approximately linear. The substituent effects on variations in the potency were similar between in-vitro and larvicidal activities. An inhibitor of oxidative detoxication, piperonyl butoxide, had no synergistic effects on the in-vitro potency. The ability of some dibenzoylhydrazines to inhibit GluNAc incorporation at exposure periods longer than the optimum for stimulation was also measured in a similar cultured integument system. The relationship between the inhibitory and stimulatory potency indices was linear, indicating that the larvicidal activity of dibenzoylhydrazines is closely related to its ability to stimulate as well as to inhibit GluNAc incorporation into the larval cuticle.     

Initial shapes of stress-strain curve of wood specimen subjected to repeated combined compression and vibration stresses and the piezoelectric behavior

This study investigated the relationship between the initial shape of the stress (σ)-strain (ε) curve of a Chamaecyparis obtusa wood specimen subjected to repeated combined compression and vibration stresses at various angles between the fiber direction and load direction and the piezoelectric behavior. The main findings of the study are: (1) the σ-ε curve became convex initially, and then the stress was proportional to the strain. The σ-ε curve had almost the same shape during both loading and unloading. (2) The σ-piezoelectric voltage (P) curve was nonlinear, with a maximal point or cusp on the curve, which had almost the same shape during both loading and unloading, as was also observed for the σ-ε curve. (3) The plot of the first derivative of the stress [dσ/dε (= σ′)] against ε was nonlinear. The σ′-ε and P-ε curves at various angles were fairly similar. (4) The stress at the maximal point (or cusp) of the σ-P curve decreased with an increase in the angle between the fiber direction and load direction. The tendency of the stresses was very similar to that of Young’s modulus and compression strength calculated from Hook’s law and Hankinson’s law, respectively.     

Mechanical behavior of the crystal lattice of natural cellulose in wood under repeated uniaxial tension stress in the fiber direction

This study investigated the relationship between the cellulose crystal lattice strain (crystalline region) and the macroscopic surface strain in specimens of Chamaecyparis obtusa wood under repeated uniaxial tension stress in the fiber direction. Changes in the strain of the crystal lattice were measured from the peak of (004) reflection using the transit X-ray method. The macroscopic surface strain of each specimen was measured with a strain gauge. In both loading and unloading, the surface strain changed linearly with changes in stress. However, crystal lattice strain was not linear but exhibited changes along a curve with changing stress. Under stressed conditions, the crystal lattice strain was always less than the surface strain, regardless of the frequency of repetition in the loading and unloading cycle. The ratio of the crystal lattice strain to the surface strain showed a negative correlation for stress in both loading and unloading. That is, the ratio decreased with increasing stress, and finally tend to converge to a specific value. The ratio (I/I ₀) between the diffracted intensity (I ₀) in the (004) plane in the unloaded condition and the diffracted intensity (I) in the (004) plane in the loaded condition tend to converge on a specific value with increasing frequency of repetition. When the substantial tension Young’s modulus of the wood in the longitudinal direction decreased, the ratio of the strain of the crystal lattice to the surface strain also decreased. Moreover, the ratio decreased with increasing microfibril angle of the specimen.