Impact of agroforestry intervention on farm income under the subsistence farming system of the middle hills, Nepal

Practices that minimize the rate of soil degradation, increase crop yields and raise farm income are key to sustaining agricultural productivity in the hills of Nepal. The use of farmland is undergoing rapid changes in response to increasing population pressure, deforestation and subsistence needs. Against this background, this study examined the impact of an agroforestry intervention project on farm income based on a sample of subsistence farm households in Dhadhing district. The project was implemented by Nepal Agroforestry Foundation in 1993/94 to increase fodder production through the promotion of agroforestry. A total of 223 households (82 with project and 141 without project) were interviewed during May–October 1998 to collect information on production and agroforestrys' impact on farm income. The benefit-cost analysis showed that the agricultural system including agroforestry was more profitable than the conventional one. The results also showed that the introduction of mulberry trees for sericulture could further enhance the profitability of an agroforestry-based system. Thus, agroforestry has great potential for enhancing food production and farmers' economic conditions in a sustainable manner through its positive contributions to household income.This revised version was published online in November 2005 with corrections to the Cover Date.     

Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization

Fucoidans, sulfated polysaccharides extracted from brown algae, are marine products with the potential to modulate bone formation and vascularization processes. The bioactivity and safety of fucoidans are highly associated with their chemical structure, which may vary with algae species and extraction method. Thus, in depth evaluation of fucoidan extracts in terms of endotoxin content, cytotoxicity, and their detailed molecular biological impact on the individual cell types in bone is needed. In this study, we characterized fucoidan extracts from three different Fucus species including Fucus vesiculosus (Fv), Fucus serratus (Fs), and Fucus distichus subsp. evanescens (Fe) for their chemical features, endotoxin content, cytotoxicity, and bioactive effects on human outgrowth endothelial cells (OEC) and human mesenchymal stem cells (MSC) as in vitro models for bone function and vascularization. Extracts contained mainly high molecular weight (HMW) fucoidans and were free of endotoxins that may cause inflammation or influence vascularization. OEC tolerated fucoidan concentrations up to 200 µg/mL, and no indication of cytotoxicity was observed. The inflammatory response, however, investigated by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) and endothelial barrier assessed by impedance measurement differed for the individual extracts. MSC in comparison with endothelial cells were more sensitive to fucoidans and showed partly reduced metabolic activity and proliferation at higher doses of fucoidans. Further results for MSC indicated impaired osteogenic functions in alkaline phosphatase and calcification assays. All tested extracts consistently lowered important molecular mediators involved in angiogenesis, such a VEGF (vascular endothelial growth factor), ANG-1 (angiopoietin 1), and ANG-2 (angiopoietin 2), as indicated by RT-PCR and ELISA. This was associated with antiangiogenic effects at the functional level using selected extracts in co-culture models to mimic bone vascularization processes during bone regeneration or osteosarcoma.     

Contribution of Small-Scale Agroforestry Systems to Carbon Pools and Fluxes: A Case Study from Middle Hills of Nepal

In view of the heavy people’s dependence (80 %) on various forms of land-based resources, carbon sequestration should not only be targeted in forests, but also on private land agroforestry. A survey was conducted in 2011 to investigate the gap in contribution of agroforestry carbon to the household economy in the middle hills region of Rasuwa district of Nepal. A total of 120 households were randomly selected and surveyed, of which eight were further examined for detailed tree carbon measurement. It is estimated that a total of 48.60 ton C per hectare has been stocked in agroforestry sites in the middle hills region. Assuming a carbon price of $US12/ton, the total potential income from carbon sequestration per household would amount to NPR 45,490/ha in 20 years of agroforestry if a payment scheme were introduced. The income from carbon sequestration is quite low compared with other agroforestry income. Policy implications are thus oriented towards farmers reaping multiple benefits from the existing international mechanisms by having negotiations based on contribution of all agroforestry components (farm trees, crops and animals) rather than limited to forest carbon stock. To benefit from these multiple functions of farms and forests, the policy framework to address the climate-related affects and risks (e.g., of landslides, burst of Himalayan lakes) should be broad enough to produce potential synergy between the negative effect of climate change and agroforestry income.     

Spatial patterns of soil microbial communities and implications for precision soil management at the field scale

Precision Agriculture - Understanding the spatial patterns of soil microbial communities and influencing factors is a prerequisite for soil health assessments and site-specific management to...     

Genotypic and phenotypic characterization of Phytophthora infestans populations on Java,Indonesia

Phytophthora infestans is endemic to Indonesia and can infect potato crops at any stage in the growing season. Little is known about P. infestans populations in Indonesia. The objectives of this study were first to identify the genotypes causing late blight in the main potato-growing regions on Java in Indonesia, and secondly to examine genotypic diversity in the P. infestans populations in those regions. Samples were collected on FTA cards (n = 140) or in tubers (n = 6) from 15 locations in nine regencies over four years (2016–2019). Microsatellite analysis revealed that late blight outbreaks in these regencies were caused by EU_2_A1 and other genotypes that are unique to Indonesia. Eighty percent of the samples that amplified with CAPS markers were the A1 mating type. Cultures of six isolates were determined to be the A1 mating type based on the pairing test, and of these, two isolates were intermediate and four were sensitive to metalaxyl-M (mefenoxam). The mode of reproduction of the P. infestans population on Java, Indonesia, was found to be clonal. However, as the sample size in this study was small, more isolates need to be tested to confirm this. Microsatellite analysis revealed that 90% of Indonesian samples had trisomic loci. A high number of multilocus genotypes (MLGs) were found in all nine regencies (131 MLGs out of 146 samples). Results indicate that there is ongoing polyploidization in these populations due to a high mutation rate and no selection pressure from the susceptible potato hosts that are being grown in Indonesia.