Effect of genistein on IGF‐1 and IGFBP‐1 in young and aged female rat ovary

The objective of this study was to assess the effects of genistein (GEN) on expression of insulin‐like growth factor 1 (IGF‐1) and insulin‐like growth factor binding protein 1 (IGFBP‐1) in young and aged rat ovary. Forty young female Sprague Dawley (SD) rats (200 ± 20 g) and forty aged female SD rats (490 ± 20 g) were selected and according to weight, they were divided into the following five groups with eight animals in each: negative control group (NC), low‐dose group (L), middle‐dose group (M), high‐dose group (H) and positive control group (PC). GEN group received GEN of 15, 30, 60 mg/kg respectively. It lasted 30 days. Concentrations of serum hormones, IGF‐1 and IGFBP‐1 were determined by enzyme‐linked immunosorbent assay (ELISA). Gene and protein expressions of IGF‐1 and IGFBP‐1 were determined by real‐time PCR and Western blot respectively. Compared with NC, GEN significantly increased oestradiol‐17β(E₂) level in aged rat, reduced luteinizing hormone (LH) level in young and aged rat. Serum levels of IGFBP‐1 in young rats were significantly higher in GEN groups (p < 0.05). mRNA and protein expression levels of IGF‐1 and IGFBP‐1 were positively correlated with GEN dose. GEN could significantly reduce the ratio of IGF‐1/IGFBP‐1 of aged rats. Multivariate Cox regression analysis result showed IGF‐1 and IGFBP‐1 levels significantly correlated with GEN dose. We speculate that there is an association between the addition of GEN and expression of IGF‐1 and IGFBP‐1, and the relationship between them is different in young and aged rat.     

托起中国军犬事业明天的太阳——走近崔金柱主任和他的军犬基地

1985～2007年,22年的时间在历史长河中瞬间而过.但对于北京军犬繁育训练基地来说,22年是从无到有,艰苦创业的如歌岁月.经过22年风风雨雨的艰苦创业,北京军犬繁育训练基地由单一的军犬训练大队发展成为保障全军军犬工作开展的,集军犬繁育、训练、教学和科研为一体的综合性基地.……     

建植第1年刈割对黑麦草+白三叶草地杂草空间格局的影响

栽培草地稳定性是近年来草地建植与管理的核心问题之一,其中草地抗杂草入侵是其稳定性的重要表现形式。本研究应用地统计学分析方法,分析了刈割处理下多年生黑麦草(Lolium perenne)+白三叶（Trifolium repens）混播草地杂草生物量空间分布的异质性。结果表明,不同刈割强度下,多年生黑麦草+白三叶混播草地入侵杂草地上生物量空间格局异质化程度及其相关范围变化比较明显。变异函数的基台值(C0+C)在不同刈割强度下的变化表明,在重度刈割条件下,杂草地上生物量空间分布异质性最高(12.530),对照条件下次之(4.751),轻度刈割条件下较低(4.557),中度刈割条件下最低(3.149)。变异函数的相关范围(A0)表明,在重度刈割条件下空间相关范围最大(1 004.1 cm),中度刈割条件下最小(122.1 cm),轻度刈割 (161.9 cm)和对照(219.1 cm)条件下居中。空间变异比分析表明,由空间自相关因素引起的空间异质性占主要部分(50.3%～93.5%)。空间分布格局图(Kriging map)分析进一步显示,不同刈割强度下多年生黑麦草+白三叶混播草地杂草地上生物量格局变化显著。     

Cholinesterase activities in uterus of normal and fenchlorphos treated blue foxes (Alopex lagopus) during various reproductive states

The uterine acetylcholinesterase and total cholinesterase (acetylcholinesterase plus butyrylcholinesterase) activities in normal and fenchlorphos treated blue fox vixens were determined during various reproductive states.AChE and Total-ChE of non-medicated vixens in oestrus were about one half of those in anoestrus. In pregnant uteri (luteal phase) the activities were 25 % and 30% compared to anoestrus.In vixens given 100 mg/kg fenchlorphos for 3 weeks during anoestrus, the remaining activity of AChE in uterus were in average 37%. Pregnant and non-pregnant vixens in the luteal phase medicated prior to mating and during time of implantation, displayed AChE activities which were only moderabely reduced (remaining activities 83% and 72% compared to medicated animals in anoestrus: remaining activity 37%).Plasma ChE-activity increased during pregnancy in the controls while enzyme activity was strongly reduced in animals given 100 mg/kg fenchlorphos daily through the whole pregnancy.It was concluded that the previous reported embryotoxic effect of fenchlorphos in the blue fox did not seem to be directed towards the moderate inhibition of the uterine cholinesterases.     

E．stiedai感染兔免疫器官内球虫抗原的免疫组化定位

用免疫组化SABC法,对斯氏艾关耳球虫感染后兔肝脏、脾脏和肝门淋巴结中球虫抗原的分布进行了观察。结果表明,在肝脏窦状隙、胆管上皮细胞、肝门淋巴结和脾脏内可见到明显的呈棕黄色阳性反应区,表明在这些区域和部位有相应的E．stiedai孢子囊、裂殖子或子孢子抗原存在,且抗原数量以肝脏内最多,其次为脾脏,最少者为肝门淋巴结;显微镜下还可见到大量坏死的肝细胞、淋巴细胞以及朗罕氏巨细胞,说明多核巨细胞也参与了对球虫的吞噬和处理过程。     

退化梯度对克氏针茅种群遗传分化的影响

对重度退化、轻度退化和正常样地的克氏针茅种群进行RAPD研究。结果表明,3个种群扩增条带总数、多态位点比率、种群内稀有位点数、遗传多样性和基因多样性均是正常种群>轻度退化种群>重度退化种群。用Shannon指数估算3个种群的遗传多样性,其中72.39%的遗传变异存在于种群内,26.89%存在于种群间。重度退化与轻度退化种群,重度退化与正常种群间的遗传相似系数分别为0.8993和0.8159。在重度退化情况下克氏针茅种群遗传结构发生了一定的遗传分化。     

双烯雌酚残留检测方法研究进展

双烯雌酚是一种人工合成的雌激素,因其残留对人体有明显的毒害,故而其在相关动物性产品中的残留受到世界各国的共同关注。目前,双烯雌酚残留检测方法主为仪器分析法,但其繁琐的分析步骤不适合药物的及时监控,而免疫学分析方法以其耗费低、特异强、灵敏高等优点,可作为兽药残留的筛选方法,因而具有广阔的应用前景。     

MTT比色法在传染性法氏囊病病毒分离中的应用

应用MTT比色法检测了病料中传染性法氏囊病病毒(IBDV)在细胞培养物中的增殖情况，并对病料中加入法氏囊提取液、鸡胚浸出液2种成分对病毒增殖的影响作了比较。结果表明，MTT比色法简便、快速、可靠，能很好地反映病毒在细胞培养物中的增殖情况。     

舍饲育肥条件下凉山半细毛羊的产肉性能研究

对三组凉山半细毛羊的 4月龄断奶公羔 ,用 3种不同的补饲水平 (Ⅰ、Ⅱ、Ⅲ )进行舍饲 ,分别于 6月龄和 8月龄对各组肥羔进行屠宰测定。结果表明高营养水平组 (Ⅰ )的羔羊 6月龄和 8月龄体质量、日增质量极显著高于低营养水平组 (Ⅲ ) (P <0 .0 1) ,显著高于中等营养水平组 (Ⅱ ) (0 .0 1

0 .0 5 )。凉山半细毛羊肌肉中氨基酸种类齐全 ,含量较丰富 ,其中必需氨基酸的总量达到 2 6 .15 %。     

新城疫（ND）—减蛋综合征（EDS76）蜂胶二联灭活疫苗的研制Ⅰ．制苗工艺、疫苗安全性和免疫原性试验

将新城疫病毒（NDV）Colon-30株和减蛋综合征病毒（EDS76AV）－127株分别接种于非免疫鸡胚和鸭胚，制备抗原液。抗原液经甲醛灭活后，与纯化的天然免疫增强剂蜂胶乳化，制成鸡新城疫（ND）－减蛋综合征（EDS76）蜂胶二联灭活疫苗，对疫苗的安全性和免疫原性进行了测定。对3－6周龄易感雏鸡以2倍使用剂量肌内注射，安全性良好；对1日龄雏鸡接种1个使用剂量进行急性毒性试验。15d生长曲线表明，对雏难 影响。对6周龄雏鸡注射1个使用剂量，每批疫苗免疫10只鸡，免疫后5－32d，鸡血清中EDS76血凝抑制（HI）抗体的几何平均滴度（GMT）为6．5－11．8log2,ND血凝抑制（HI）抗体的几何平均滴度（GMT）为4．8－9．8log2；对ND免疫攻毒试验表明，3批疫苗，每羽份含有的半数保护量（PD50）分别为≥127、≥131和≥127。