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301.
Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.  相似文献   
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Chen S  Zhang YE  Long M 《Science (New York, N.Y.)》2010,330(6011):1682-1685
To investigate the origin and evolution of essential genes, we identified and phenotyped 195 young protein-coding genes, which originated 3 to 35 million years ago in Drosophila. Knocking down expression with RNA interference showed that 30% of newly arisen genes are essential for viability. The proportion of genes that are essential is similar in every evolutionary age group that we examined. Under constitutive silencing of these young essential genes, lethality was high in the pupal stage and also found in the larval stages. Lethality was attributed to diverse cellular and developmental defects, such as organ formation and patterning defects. These data suggest that new genes frequently and rapidly evolve essential functions and participate in development.  相似文献   
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M?ssbauer spectra of several iron meteorites have been measured by a resonant scattering technique rather than by the conventional transmission method, thereby eliminating the necessity for the preparation of thin samples. No significant differences were observed in the spectra of specimens of mechanically deformed, shocked, and unshocked iron meteorites, nor in the absorption spectra of artificially shocked and unshocked fayalite.  相似文献   
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The present acidity and sulfur content of the upland streams of New England can be accounted for on the basis of the rainout and washout of sulfuric acid from the atmosphere. In spite of this excess acid flux over the New England landscape, the rate of cationic denudation of upland watersheds is relatively low.  相似文献   
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A major probable impact structure occurs in middle Proterozoic dacitic volcanics in the Gawler Ranges, central South Australia. The structure has an inner depressed area about 30 kilometers in diameter that contains the Lake Acraman salina, an intermediate depression or ring about 90 kilometers in diameter, and a possible outer ring approximately 160 kilometers in diameter. Outcrops of dacite in Lake Acraman are intensely shattered and contain shatter cones and multiple sets of shock lamellac in quartz grains. The Acraman structure is the largest probable impact structure known in Australia and is the likely source of dacitic ejecta found in late Precambrian marine shales some 300 kilometers to the east.  相似文献   
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